{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T15:21:30Z","timestamp":1788189690114,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,9,5]],"date-time":"2025-09-05T00:00:00Z","timestamp":1757030400000},"content-version":"vor","delay-in-days":247,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["316447\/2021-8"],"award-info":[{"award-number":["316447\/2021-8"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["126267\/2025-1"],"award-info":[{"award-number":["126267\/2025-1"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:italic>Erysipelothrix<\/jats:italic>\n                    (\n                    <jats:italic>E<\/jats:italic>\n                    .)\n                    <jats:italic>piscisicarius<\/jats:italic>\n                    is an emerging pathogen previously described in fish and more recently isolated from a clinical outbreak in swine. This study aimed to evaluate the course of infection and pathological outcomes of\n                    <jats:italic>E. piscisicarius<\/jats:italic>\n                    in pigs using an experimental intradermal challenge model. Twenty\u2010six 70\u2010day\u2010old pigs were randomly allocated into three groups: high\u2010dose (HD group,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200910) and low\u2010dose (LD group,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200910) were challenged intradermally with 10\n                    <jats:sup>10<\/jats:sup>\n                    colony forming units (CFUs) and 10\n                    <jats:sup>8<\/jats:sup>\n                    CFU using a Brazilian field isolate obtained from a pig with erysipelas\u2010like lesions and confirmed by whole\u2010genome sequencing (WGS) and average nucleotide identity (ANI), respectively, while CONT (control,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096) served as a negative control. Clinical monitoring, hematological assessments, acute\u2010phase proteins (APPs) quantification, bacteriological culture, and quantitative PCR (qPCR) targeting the\n                    <jats:italic>spaC<\/jats:italic>\n                    gene were performed over 14 days. Challenged pigs developed mild clinical signs, including transient fever and characteristic rhomboid skin lesions resembling classical swine erysipelas. No mortality occurred. Hematological analysis revealed significant reductions in red blood cell (RBC) count, hemoglobin (HGB), and hematocrit (HCT), particularly in the LD group at 7 and 14 days post\u2010challenge (dpc) (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009 &lt; 0.05), suggestive of inflammatory anemia. APP analysis showed a significant increase in ceruloplasmin across all groups over time, whereas transferrin levels decreased only in the control group. Bacterial isolation was unsuccessful; however, qPCR detected\n                    <jats:italic>E. piscisicarius<\/jats:italic>\n                    deoxyribonucleic acid (DNA) in blood, skin, liver, and spleen samples, confirming systemic dissemination, particularly at 7\u2009dpc. These findings demonstrate that\n                    <jats:italic>E. piscisicarius<\/jats:italic>\n                    can induce clinical and pathological alterations in swine, although with mild severity under experimental conditions. Moreover, the study highlights the importance of differentiating\n                    <jats:italic>E. piscisicarius<\/jats:italic>\n                    from\n                    <jats:italic>E. rhusiopathiae<\/jats:italic>\n                    in diagnostics, given the potential limitations of current vaccine strategies.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/5618952","type":"journal-article","created":{"date-parts":[[2025,9,6]],"date-time":"2025-09-06T06:05:32Z","timestamp":1757138732000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Pathogenic Potential of\n                    <i>Erysipelothrix piscisicarius<\/i>\n                    in Pigs and Its Implications for Surveillance in Brazil"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1140-7749","authenticated-orcid":false,"given":"Fernando Moreira","family":"Petri","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2303-3161","authenticated-orcid":false,"given":"Giovana da Silva","family":"Nogueira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo M. R.","family":"Sim\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8795-3629","authenticated-orcid":false,"given":"Ana K.","family":"Panneitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3745-4589","authenticated-orcid":false,"given":"Gabriel A. de","family":"Aguiar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6517-2759","authenticated-orcid":false,"given":"Ana Clara A. de","family":"Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1937-1947","authenticated-orcid":false,"given":"Eduarda R.","family":"Braga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kellem do","family":"Carmo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8834-8129","authenticated-orcid":false,"given":"Suzana S.","family":"Kuchiishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrienny T.","family":"Reis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8901-7356","authenticated-orcid":false,"given":"Fabio A.","family":"Vannucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1861-5076","authenticated-orcid":false,"given":"Lu\u00eds Guilherme","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,5]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0103-84782007000500043"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1590\/1678-5150-pvb-6997"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.003838"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-82959-x"},{"key":"e_1_2_13_5_2","volume-title":"Berge\u2019s Manual of Determinative Bacteriology: The Firmicutes","author":"Whitman W.","year":"2010"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.00174"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00840-23"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.006264"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/9781119350927.ch53"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-37-2-166"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063871102300126"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12673"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13485"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2022.2140614"},{"key":"e_1_2_13_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2009.04560.x"},{"key":"e_1_2_13_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2010.04746.x"},{"key":"e_1_2_13_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-017-0844-4"},{"key":"e_1_2_13_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-10210-3"},{"key":"e_1_2_13_19_2","unstructured":"RebelattoR. KleinC. S. SurianC. R. KichJ. D. CaronL. andMoresM. A. Z. Experimental Model: Reproduction of Erysipelas in Pigs International Pig Veterinary Society Congress 2022 Rio de Janeiro IPVS: Abraves Proceedings. Rio de Janeiro RJ:."},{"key":"e_1_2_13_20_2","first-page":"683","article-title":"A Rapid, Easy and High Yield Protocol for Total Genomic DNA Isolation of Colletotrichum gloeosporioides and Fusarium oxysporum","volume":"19","author":"Kuramae-Izioka E. E.","year":"1997","journal-title":"Unimar"},{"key":"e_1_2_13_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_13_22_2","first-page":"3","volume-title":"Diseases of Swine","author":"Dewey C. 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