{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T06:17:15Z","timestamp":1788157035445,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"vor","delay-in-days":241,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005621","name":"Kasetsart University Research and Development Institute","doi-asserted-by":"publisher","award":["FF(KU-SRIU)16.67"],"award-info":[{"award-number":["FF(KU-SRIU)16.67"]}],"id":[{"id":"10.13039\/501100005621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    The genus\n                    <jats:italic>Erysipelothrix<\/jats:italic>\n                    comprises facultative anaerobic, nonspore\u2010forming, gram\u2010positive bacteria that can cause skin infections and severe diseases such as septicemia and endocarditis in humans. Although\n                    <jats:italic>E. rhusiopathiae<\/jats:italic>\n                    is the primary pathogen, other species may also be involved, necessitating accurate identification. However, 16S rDNA sequencing lacks sufficient resolution to differentiate among\n                    <jats:italic>Erysipelothrix<\/jats:italic>\n                    species. In this study, we used Oxford Nanopore Technology (ONT) to directly sequence low\u2010quality DNA extracted from heart valve tissue of a 66\u2010year\u2010old female patient with a fatal case of septicemia and aortic endocarditis. In contrast to 16S rDNA Illumina sequencing and matrix\u2010assisted laser desorption ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF MS), which incorrectly identified the pathogen as\n                    <jats:italic>E. rhusiopathiae<\/jats:italic>\n                    , direct sequencing via ONT precisely identified\n                    <jats:italic>E. piscisicarius<\/jats:italic>\n                    as the cause of infection. About 1.47\u2009Mb genome was retrieved from nanopore direct sequencing. Within the\n                    <jats:italic>E. piscisicarius<\/jats:italic>\n                    genome, we detected genes associated with virulence. Phylogenetic analysis showed that our strain clustered with a human\u2010derived\n                    <jats:italic>E. piscisicarius<\/jats:italic>\n                    strain from China and swine\u2010derived strains from Brazil. In conclusion, this study demonstrated that ONT can be used to sequence low\u2010quality DNA extracted directly from patient specimens, obtain a draft bacterial genome, and reliably distinguish between pathogenic species.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/5654145","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T06:00:02Z","timestamp":1788156002000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Oxford Nanopore Sequencing of Clinical DNA for Identification and Comparative Genomic Analysis of\n                    <i>Erysipelothrix piscisicarius<\/i>"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5067-5389","authenticated-orcid":false,"given":"Rattagan","family":"Kajeekul","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3659-2074","authenticated-orcid":false,"given":"Thidathip","family":"Wongsurawat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0421-0211","authenticated-orcid":false,"given":"Worarat","family":"Kruasuwan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phichaya","family":"Sawankatat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pattaraporn","family":"Nimsamer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tantip","family":"Arigul","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Athita","family":"Riwlord","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1555-401X","authenticated-orcid":false,"given":"Piroon","family":"Jenjaroenpun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1055-3656","authenticated-orcid":false,"given":"Anusak","family":"Kerdsin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,30]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.003838"},{"key":"e_1_2_13_2_2","doi-asserted-by":"crossref","DOI":"10.1099\/ijsem.0.005454","article-title":"Erysipelothrix anatis sp. 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