{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:40:40Z","timestamp":1773009640283,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T00:00:00Z","timestamp":1764720000000},"content-version":"vor","delay-in-days":336,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32202889"],"award-info":[{"award-number":["32202889"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32302879"],"award-info":[{"award-number":["32302879"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172872"],"award-info":[{"award-number":["32172872"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Leptospirosis, a globally re\u2010emerging zoonosis caused by pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    species, poses escalating public health challenges in rapidly urbanizing regions. Canines, as significant reservoir hosts, are increasingly regarded as effective sentinels for human leptospirosis risk. This study assessed the seroprevalence of pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    in dogs across multiple provinces and regions along the Yangtze River in China. From 2021 to 2023, a total of 1517 canine serum samples were collected from Shanghai, Jiangsu, Anhui, Jiangxi, Hunan, Hubei, Chongqing, Sichuan, and Yunnan. In addition, a tissue sample was obtained from an infected dog, leading to the successful isolation and culture of one\n                    <jats:italic>Leptospira<\/jats:italic>\n                    strain. Microscopic agglutination test (MAT) results indicated an overall seroprevalence of 46.41% (704\/1517), predominantly involving\n                    <jats:italic>L. interrogans<\/jats:italic>\n                    serogroups Canicola (72.73%, 512\/704) and Icterohaemorrhagiae (28.68%, 202\/704), followed by Ballum (18.04%, 127\/704) and Australis (17.90%, 126\/704). Organ examination and histopathological analysis identified severe pulmonary hemorrhage induced by the isolated strain as the primary cause of canine mortality. Whole\u2010genome sequencing (WGS) and multilocus sequence typing (MLST) based on seven housekeeping genes classified the isolate as\n                    <jats:italic>L. interrogans<\/jats:italic>\n                    serovar Australis, sequence type (ST) ST93. These findings reveal a high seroprevalence of pathogenic\n                    <jats:italic>Leptospira<\/jats:italic>\n                    in dogs within the Yangtze River region, consistent with the distribution of locally prevalent serogroups, and underscore the potential public health risk posed by this zoonotic pathogen in the area.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/5728490","type":"journal-article","created":{"date-parts":[[2025,12,3]],"date-time":"2025-12-03T08:45:59Z","timestamp":1764751559000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Seroprevalence and Molecular Epidemiology of\n                    <i>Leptospira<\/i>\n                    spp. Infecting Dogs in the Yangtze River Region of China"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5348-6501","authenticated-orcid":false,"given":"Yue","family":"Ding","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2966-5140","authenticated-orcid":false,"given":"Shilei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6313-2241","authenticated-orcid":false,"given":"Wenlong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6468-4809","authenticated-orcid":false,"given":"Sheng","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4632-0443","authenticated-orcid":false,"given":"Xufeng","family":"Xie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9533-7516","authenticated-orcid":false,"given":"Yongguo","family":"Cao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,3]]},"reference":[{"key":"e_1_2_11_1_2","first-page":"65","article-title":"Leptospirosis in humans","volume":"387","author":"Haake D. A.","year":"2015","journal-title":"Current Topics in Microbiology and Immunology"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00830-2"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003898"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1012161"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.onehlt.2025.101016"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007811"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2019.2544"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-45059-8"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40249-018-0435-2"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11091040"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani12141788"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.607115"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1995-7645(11)60205-8"},{"key":"e_1_2_11_14_2","first-page":"280","article-title":"Serological Survey of Canine Leptospirosis in Southern china","volume":"32","author":"Shi D. Y.","year":"2012","journal-title":"Pakistan Veterinary Journal"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.22092\/ari.2020.127253.1376"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2010.07.008"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40249-025-01284-x"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2019.06.004"},{"key":"e_1_2_11_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/zph.12549"},{"key":"e_1_2_11_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.138251"},{"key":"e_1_2_11_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0007513"},{"key":"e_1_2_11_22_2","first-page":"422","article-title":"The Spatiotemporal Epidemiology and Influencing Factor Analysis of Leptospirosis - Anhui Province, China, 2004-2023","volume":"7","author":"Lian Y.","year":"2025","journal-title":"China CDC Weekly"},{"key":"e_1_2_11_23_2","doi-asserted-by":"publisher","DOI":"10.7589\/2019-01-009"},{"key":"e_1_2_11_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11010099"},{"key":"e_1_2_11_25_2","doi-asserted-by":"publisher","DOI":"10.16250\/j.32.1374.2024033"},{"key":"e_1_2_11_26_2","unstructured":"Organization wh (2003) Human Leptospirosis: Guidance for Diagnosis Surveillance and Control 2003."},{"key":"e_1_2_11_27_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens12070930"},{"key":"e_1_2_11_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0111367"},{"key":"e_1_2_11_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.07.070"},{"key":"e_1_2_11_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-0459-5"},{"key":"e_1_2_11_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0010589"},{"key":"e_1_2_11_32_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001954"},{"key":"e_1_2_11_33_2","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.14826.1"},{"key":"e_1_2_11_34_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0000000000000097"},{"key":"e_1_2_11_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2011.11.007"},{"key":"e_1_2_11_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2019.02.008"},{"key":"e_1_2_11_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-015-0341-9"},{"key":"e_1_2_11_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2023.105869"},{"key":"e_1_2_11_39_2","doi-asserted-by":"publisher","DOI":"10.4067\/S0716-10182013000300003"},{"key":"e_1_2_11_40_2","first-page":"366","article-title":"Epidemiology Development Surveillance of Leptospirosis in Chongqing Section of Three Gorges Dam Area","volume":"28","author":"Long J.","year":"2007","journal-title":"Chinese Journal of Epidemiology"}],"container-title":["Transboundary and Emerging Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tbed\/5728490","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/tbed\/5728490","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tbed\/5728490","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:54:47Z","timestamp":1773003287000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/tbed\/5728490"}},"subtitle":[],"editor":[{"given":"Zongfu","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":40,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1155\/tbed\/5728490"],"URL":"https:\/\/doi.org\/10.1155\/tbed\/5728490","archive":["Portico"],"relation":{},"ISSN":["1865-1674","1865-1682"],"issn-type":[{"value":"1865-1674","type":"print"},{"value":"1865-1682","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2025-04-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"5728490"}}