{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T16:27:00Z","timestamp":1775060820392,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"vor","delay-in-days":55,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100018914","name":"Fujian Academy of Agricultural Sciences","doi-asserted-by":"publisher","award":["XTCXGC2021018"],"award-info":[{"award-number":["XTCXGC2021018"]}],"id":[{"id":"10.13039\/501100018914","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018914","name":"Fujian Academy of Agricultural Sciences","doi-asserted-by":"publisher","award":["XTCXGC2021012"],"award-info":[{"award-number":["XTCXGC2021012"]}],"id":[{"id":"10.13039\/501100018914","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2025J011234"],"award-info":[{"award-number":["2025J011234"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Muscovy duck reovirus (MDRV) causes substantial economic losses in the waterfowl industry, necessitating rapid and field\u2010deployable diagnostic tools. In this study, we developed, for the first time, a lyophilized reagent\u2010based reverse transcription recombinase\u2010aided amplification (RT\u2010RAA) assay targeting the \u03c3C gene of MDRV for point\u2010of\u2010care testing (POCT). The assay was optimized to operate at 39\u00b0C with a probe concentration of 100\u2009nM. It achieved a detection limit of 1.03\u2009\u00d7\u200910\n                    <jats:sup>1<\/jats:sup>\n                    copies\/\u03bcL within 20\u2009min, which is comparable to RT\u2010quantitative polymerase chain reaction (qPCR) and superior to existing methods. It exhibited high specificity for MDRV with no cross\u2010reactivity against other common waterfowl pathogens. Particularly, the assay was successfully lyophilized into a ready\u2010to\u2010use format, representing the first reported RT\u2010RAA\u2010based method for MDRV detection in such a format. The lyophilized reagents retained the key analytical performance characteristics without cold\u2010chain dependance. When evaluated on 42 clinical samples, the lyophilized RT\u2010RAA demonstrated 95.8% diagnostic sensitivity and 100% specificity compared to RT\u2010qPCR, with almost perfect agreement (\n                    <jats:italic>\u03ba<\/jats:italic>\n                    \u2009=\u20090.952). A visual readout using a portable blue light imager further enabled naked\u2010eye interpretation without instrumentation. This robust, rapid, and field\u2010deployable RT\u2010RAA assay provides a practical solution for on\u2010site MDRV surveillance, particularly in low\u2010resource settings.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/5924181","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T00:18:32Z","timestamp":1773015512000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid and Visual Detection of Muscovy Duck Reovirus Using a Reverse Transcription Recombinase\u2010Aided Amplification Assay in a Lyophilized Format for Point\u2010of\u2010Care Applications"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4027-110X","authenticated-orcid":false,"given":"Xiuqin","family":"Chen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-2845-9882","authenticated-orcid":false,"given":"Shizhong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-6185-0044","authenticated-orcid":false,"given":"Xiaochun","family":"Luo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3647-1121","authenticated-orcid":false,"given":"Guangju","family":"You","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9781-9586","authenticated-orcid":false,"given":"Shaoying","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1491-5143","authenticated-orcid":false,"given":"Meiqing","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9432-2961","authenticated-orcid":false,"given":"Shao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6533-8905","authenticated-orcid":false,"given":"Shilong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,25]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2018.10.102"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/03079459208418866"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2013.10.004"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2000.0473"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0264-410x(02)00264-5"},{"key":"e_1_2_9_6_2","article-title":"A New Virus Disease of the Muscovy Duck (Cairina moschata Linn.) Present in Natal","author":"Kaschula V. R.","year":"1950","journal-title":"Journal of the South African Veterinary Association"},{"key":"e_1_2_9_7_2","first-page":"2916","article-title":"A New Disease Expressing Itself by a Viral Pericarditis in Barbary Ducks","volume":"274","author":"Gaudry D.","year":"1972","journal-title":"Comptes Rendus Hebdomadaires des Seances de L\u2019academie des Sciences. Serie D: Sciences Naturelles"},{"key":"e_1_2_9_8_2","first-page":"242","article-title":"The Identification of Muscovy Duck Reovirus","volume":"20","author":"Hu Q.","year":"2004","journal-title":"Chinese Journal of Virology"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2021.109286"},{"key":"e_1_2_9_10_2","first-page":"41","article-title":"Preparation and Application of Monoclonal Antibody Against Muscovy Duck Reovirus","volume":"40","author":"Zhu X.","year":"2012","journal-title":"Journal of Northwest A&F University"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2006.12.008"},{"key":"e_1_2_9_12_2","first-page":"1107","article-title":"Establishment of a RT-PCR Method for Duck Reovirus Detection","volume":"32","author":"Wang Y.","year":"2016","journal-title":"Jiangsu Academy of Agricultural Sciences"},{"key":"e_1_2_9_13_2","first-page":"83","article-title":"Establishment and Application of a Nest RT-PCR Method for Detection of Muscovy Duck Reovirus","volume":"39","author":"Li Q.","year":"2012","journal-title":"Chinese Journal of Animal Science"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2020.101575"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.8b01309"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13411"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1155\/2024\/7108960"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2024.110822"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.4c00450"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.1026129"},{"key":"e_1_2_9_21_2","doi-asserted-by":"crossref","DOI":"10.1002\/adhm.202500522","article-title":"Thermodynamic Delivery of Trehalose Into Extracellular Vesicles for Enhanced Lyophilization","volume":"15","author":"Wang T.","year":"2025","journal-title":"Advanced Healthcare materials"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-024-61163-7"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2024.03.004"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biosx.2025.100635"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.26508\/lsa.202403167"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13849"},{"key":"e_1_2_9_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.14435"},{"key":"e_1_2_9_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.12.001"},{"key":"e_1_2_9_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2025.117428"},{"key":"e_1_2_9_30_2","doi-asserted-by":"publisher","DOI":"10.2174\/1389557522666220802144057"},{"key":"e_1_2_9_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202509355"}],"container-title":["Transboundary and Emerging Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tbed\/5924181","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/tbed\/5924181","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tbed\/5924181","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T14:49:17Z","timestamp":1775054957000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/tbed\/5924181"}},"subtitle":[],"editor":[{"given":"Nanhua","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":31,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/tbed\/5924181"],"URL":"https:\/\/doi.org\/10.1155\/tbed\/5924181","archive":["Portico"],"relation":{},"ISSN":["1865-1674","1865-1682"],"issn-type":[{"value":"1865-1674","type":"print"},{"value":"1865-1682","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-12-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"5924181"}}