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This study investigated the prevalence and subtypes of\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    and\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    in cattle from six farms across three provinces and one autonomous region in northeast China. Fecal samples (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009495) were detected using PCR\u2010based methods:\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    was genotyped via PCR\u2010restriction fragment length polymorphism (RFLP) targeting the small subunit ribosomal RNA (\n                    <jats:italic>SSU<\/jats:italic>\n                    rRNA) gene, and\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    assemblages were identified through multilocus sequence typing of the\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010giardin (\n                    <jats:italic>bg<\/jats:italic>\n                    ), glutamate dehydrogenase (\n                    <jats:italic>gdh<\/jats:italic>\n                    ), and triose phosphate isomerase (\n                    <jats:italic>tpi<\/jats:italic>\n                    ) genes. Overall infection rates were 44.44% (220\/495) for\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    and 39.39% (195\/495) for\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    . Age\u2010related trends showed\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    prevalence peaked in preweaned calves (63.29%, 50\/79), whereas the highest prevalence rate of\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    infections (53.66%, 66\/123) was observed in calves aged 3\u201311 months. Four\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    species (\n                    <jats:italic>C. parvum<\/jats:italic>\n                    ,\n                    <jats:italic>C. bovis<\/jats:italic>\n                    ,\n                    <jats:italic>C. ryanae<\/jats:italic>\n                    , and\n                    <jats:italic>C. andersoni<\/jats:italic>\n                    ) and coinfections involving two or three\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    species were identified. Based on 60\u2010kilodalton glycoprotein (\n                    <jats:italic>gp60<\/jats:italic>\n                    ) gene of\n                    <jats:italic>C. parvum<\/jats:italic>\n                    , three subtypes (IIdA19G1, IIdA24G2, and IIdA21G1) were identified. Subtype IIdA21G1 was reported for the first time in cattle, with its initial detection occurring in China. For\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    , assemblages A and E were identified in all four areas, with 13 assemblage E multilocus genotypes (MLGs), one assemblage A MLG, and eight mixed (A\u2009+\u2009E) MLGs detected. Our findings revealed a novel genetic subtype of\n                    <jats:italic>C. parvum<\/jats:italic>\n                    in China, and the high prevalence of both\n                    <jats:italic>Cryptosporidium<\/jats:italic>\n                    and\n                    <jats:italic>G. duodenalis<\/jats:italic>\n                    suggested an increased zoonotic risk that deserved more attention.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/6148130","type":"journal-article","created":{"date-parts":[[2025,7,24]],"date-time":"2025-07-24T14:19:27Z","timestamp":1753366767000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic Characterization and Zoonotic Potential of\n                    <i>Cryptosporidium<\/i>\n                    spp. and\n                    <i>Giardia duodenalis<\/i>\n                    in Cattle From Northeast 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