{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T07:57:53Z","timestamp":1781510273241,"version":"3.54.1"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T00:00:00Z","timestamp":1766880000000},"content-version":"vor","delay-in-days":361,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100015600","name":"Guangdong Ocean University","doi-asserted-by":"publisher","award":["060302052301"],"award-info":[{"award-number":["060302052301"]}],"id":[{"id":"10.13039\/501100015600","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Porcine epidemic diarrhea virus (PEDV) is an enteric coronavirus that infects pigs of all ages. Notably, neonatal piglets (\u22647 days old) in na\u00efve herds can experience case fatality rates approaching 100%, with hallmark clinical signs of vomiting, diarrhea, and dehydration, thereby representing a major threat to swine herd health. Serum PEDV\u2010specific IgG serves as a key serological marker of vaccine\u2010induced herd immunity. Accordingly, we developed an indirect ELISA (iELISA) using Chinese hamster ovary (CHO) cell\u2010expressed recombinant PEDV spike (S) protein as the coating antigen. Checkerboard titration identified the optimal antigen\u2010coating concentration and serum dilution as 1\u2009\u03bcg\/mL and 1:300, respectively. Based on sample\u2010to\u2010positive (S\/P) ratios from 50 PEDV\u2010negative sera, the diagnostic cutoff was set at 0.796, corresponding to the mean plus 3 standard deviation (SD) of the negative control group (\n                    <jats:italic>n<\/jats:italic>\n                    =\u200950). The intra\u2010 and interassay coefficients of variation (CVs) were both &lt;6.30%, indicating good precision (repeatability) and reproducibility. Specificity testing showed no detectable cross\u2010reactivity with sera positive for five common porcine viruses (porcine deltacoronavirus (PDCoV), transmissible gastroenteritis virus (TGEV), porcine group A rotavirus (PoRVA), porcine reproductive and respiratory syndrome virus (PRRSV), and African swine fever virus (ASFV)). Moreover, PEDV\u2010specific IgG measured by iELISA correlated strongly with virus\u2010neutralizing (VN) antibody titers (\n                    <jats:italic>r<\/jats:italic>\n                    =\u20090.95), and both metrics were associated with protective outcomes at the herd level. Collectively, this assay provides an effective tool for evaluating protective immunity following PEDV vaccination.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/6185969","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T02:37:13Z","timestamp":1766975833000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Development of an Indirect ELISA Based on the PEDV S Protein and Evaluation of Its Correlation With Neutralizing Antibody and Immune Protection"],"prefix":"10.1155","volume":"2025","author":[{"given":"Guangming","family":"Ma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangli","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangcai","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wang","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-3303-2646","authenticated-orcid":false,"given":"Shuqiong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7556-2268","authenticated-orcid":false,"given":"Hanqin","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7529-0677","authenticated-orcid":false,"given":"Yuanjia","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,28]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1803.120002"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1177\/0300985815591080"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani14020294"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2020.198045"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-024-01384-w"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/v14112434"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0193682"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/v9070177"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01455-12"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/1040638713501675"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.23090"},{"key":"e_1_2_9_12_2","doi-asserted-by":"crossref","unstructured":"WeiZ.-Y. 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