{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T04:46:33Z","timestamp":1789620393557,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T00:00:00Z","timestamp":1767571200000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>African swine fever (ASF), a high\u2010profile transboundary animal disease caused by ASF virus (ASFV), imposes a devastating impact on the global swine industry. Given that vaccines are still under development, including field evaluations, early detection of ASFV is crucial for effective disease control and mitigation. Although PCR is the primary viral detection method of acute or subacute ASFV infections, antibody detection plays a unique role in detecting low\u2010virulent ASFV infection, identifying recovered animals, and tracking viral transmission. ELISA for ASFV antibody detection is commonly used for initial serological screening. To avoid false positive results, the World Organisation for Animal Health (WOAH) recommends using a second serologic method, such as the indirect immunofluorescence assay (IFA), indirect immunoperoxidase test (IPT), or immunoblot test, to confirm the ELISA\u2010positive cases. This strategy improves specificity but not sensitivity (i.e., false negative cases persist). To address this issue, a novel in\u2010cell ELISA (icELISA) was developed in this study. Receiver operating curve analysis of the icELISA revealed the optimized cutoff value of sample\u2010to\u2010positive ratio (S\/P ratio) was at 47% with 99.46% analytical sensitivity and 99.43% analytical specificity. Results of the comparative diagnostic sensitivity analysis showed that positive detections of icELISA (150 samples) surpassed a blocking ELISA\u2010IPT combination (132 samples) by 18 samples. Further investigation revealed that the 18 samples contained ASFV\u2010specific immunoglobulin M (IgM) antibodies instead of immunoglobulin G (IgG). The results suggested the icELISA can detect both ASFV\u2010specific IgG and IgM, which outperforms a blocking ELISA\u2010IPT combination in earlier detection, particularly when only IgM antibody is present in a test sample.<\/jats:p>","DOI":"10.1155\/tbed\/6272844","type":"journal-article","created":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T09:11:04Z","timestamp":1767604264000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Novel In\u2010Cell ELISA With Superior Sensitivity and Specificity for the Detection of African Swine Fever Virus\u2010Specific IgM and IgG Antibodies"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3475-6335","authenticated-orcid":false,"given":"Ping","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0548-0520","authenticated-orcid":false,"given":"Aric J.","family":"McDaniel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yelitza Y.","family":"Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7169-3954","authenticated-orcid":false,"given":"Vivian","family":"O\u2019Donnell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Jia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,5]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0368-1742(21)80031-4"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2012.11.030"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40249-018-0495-3"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/v14051090"},{"key":"e_1_2_12_5_2","first-page":"389","article-title":"A Recent Case of African Swine Fever in Kenya, East Africa","volume":"126","author":"Hammond R. 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