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To assess whether those migrants are carriers of pure or hybrid schistosomes, a complete genetic characterization of\n                    <jats:italic>Schistosoma<\/jats:italic>\n                    entering Spain is performed. A total of 759 eggs (from urine\u2009+\u2009stools) from 58 patients from 8 African countries were individually processed to describe their mito\u2010nuclear signature by\n                    <jats:italic>cox<\/jats:italic>\n                    1 rapid diagnostic multiplex one\u2010step polymerase chain reaction (RD\u2010PCR) and ITS\u20102\/18S sequencing and haplotype identification by means of the complete ITS1\u20105.8S\u2010ITS2 rDNA and\n                    <jats:italic>cox<\/jats:italic>\n                    1 sequencing. Combined nuclear and mitochondrial DNA markers in sub\u2010Saharan migrants residing in Spain are described for the first time. Twenty\u2010two (40.74%) patients were simultaneously carrying pure and hybrid eggs in their urine. S\n                    <jats:italic>chistosoma haematobium<\/jats:italic>\n                    \u2009\u00d7\u2009\n                    <jats:italic>S. bovis<\/jats:italic>\n                    (68.18%) and\n                    <jats:italic>S. haematobium<\/jats:italic>\n                    \u2009\u00d7\u2009\n                    <jats:italic>S. curassoni<\/jats:italic>\n                    (31.82%) hybrid combinations were the most frequent. Six (one pure and five hybrid) and two (pure) mito\u2010nuclear signatures, in urine and stools, respectively, and 12 nuclear and 61 mitochondrial imported haplotypes were found. This study highlights the genetic complexity of pure and hybrid schistosomes that enter Spain, and consequently Europe, and contributes to the following: correlate the geographical origin of patients with pure and\/or hybrid genetic types; detect the presence of hybrids \u201cat distance\u201d (hybrids in migrants from Guinea\u2010Bissau and Mauritania are first time detected); correlate molecular haplotypes with pathologies, clinical pictures, and treatment responses; and, importantly, warn about possible sources of autochthonous transmission.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/6614509","type":"journal-article","created":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T09:04:24Z","timestamp":1748336664000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Imported Schistosomiasis in Southwestern Europe: Wide Variation of Pure and Hybrid Genotypes Infecting Sub\u2010Saharan 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