{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T07:49:18Z","timestamp":1791532158667,"version":"4.3.4"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"vor","delay-in-days":280,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100010203","name":"Agriculture Research System of China","doi-asserted-by":"publisher","award":["CARS-41-18"],"award-info":[{"award-number":["CARS-41-18"]}],"id":[{"id":"10.13039\/501100010203","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018914","name":"Fujian Academy of Agricultural Sciences","doi-asserted-by":"publisher","award":["YCZX202412"],"award-info":[{"award-number":["YCZX202412"]}],"id":[{"id":"10.13039\/501100018914","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018914","name":"Fujian Academy of Agricultural Sciences","doi-asserted-by":"publisher","award":["DWHZ2026-04"],"award-info":[{"award-number":["DWHZ2026-04"]}],"id":[{"id":"10.13039\/501100018914","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Over the past decade, there has been an increase in reports of duck adenovirus type 3 (DAdV\u20103) infections in Muscovy ducks across various provinces in China, with mortality rates reaching as high as 43%. The rising incidence of this disease has led to significant economic losses for the waterfowl farming industry in China. In this study, a DAdV\u20103 strain was isolated from a Muscovy duck farm in Fujian province, which we designated as the FJNP\u20102021 strain, and further explored its pathogenicity and genetic characteristics. Pathogenicity analysis demonstrated that this FJNP\u20102021 strain induces characteristic pathological changes in Muscovy ducklings, exhibiting a broad tissue tropism, with the liver exhibiting the most severe lesions. The ultrastructural analysis revealed significant accumulation of lipid droplets and structurally disordered endoplasmic reticulum (ER) surrounding mitochondria in the hepatocytes of Muscovy ducklings postinfection. Moreover, in vivo DAdV\u20103 infection significantly downregulated ferroptosis suppressive genes (\n                    <jats:italic>SLC7A11<\/jats:italic>\n                    ,\n                    <jats:italic>GPX4<\/jats:italic>\n                    , and\n                    <jats:italic>FTH1<\/jats:italic>\n                    ) while upregulating proferroptotic genes\n                    <jats:italic>TP53<\/jats:italic>\n                    and\n                    <jats:italic>CHAC1<\/jats:italic>\n                    . Our study provides dual ultrastructural morphology and molecular evidence that DAdV\u20103\u2013induced hepatic lipid metabolism disorder triggers ferroptosis, thereby exacerbating hepatocyte injury. Phylogenetic analysis revealed that the complete genome sequence of the FJNP\u20102021 strain exhibited 92.3% identity with the representative strain GR from France, which was clustered in a different evolutionary lineage. Furthermore, the FJNP\u20102021 strain was grouped within the same lineage as domestic DAdV\u20103 strains, consistent with the genetic characteristics of the predominant epidemic strains of DAdV\u20103 in China. Notably, the Fiber1 genes of the Chinese DAdV\u20103 isolates formed two distinct subclades, and the Fiber1 gene of the FJNP\u20102021 strain clustered within the major subclade. In conclusion, this study analyzed the pathogenicity and genetic characteristics of all available DAdV\u20103 strains, enriching our understanding of the genomic diversity of Chinese isolates from 2014 to 2024 and providing a basis for further studies on DAdV\u20103 evolution and pathogenesis.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/7156678","type":"journal-article","created":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T06:55:35Z","timestamp":1791528935000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pathogenicity and Genomic Features of a DAdV\u20103 Strain Causing Severe Hepatitis in Muscovy Ducklings"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7148-4311","authenticated-orcid":false,"given":"Chunhua","family":"Zhu","sequence":"first","affiliation":[{"name":"Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention Institute of Animal Husbandry and Veterinary Medicine Fujian Academy of Agricultural Sciences  Fuzhou 350013 China  faas.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwang","family":"Tang","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention Institute of Animal Husbandry and Veterinary Medicine Fujian Academy of Agricultural Sciences  Fuzhou 350013 China  faas.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei","family":"Yang","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention Institute of Animal Husbandry and Veterinary Medicine Fujian Academy of Agricultural Sciences  Fuzhou 350013 China  faas.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longfei","family":"Chen","sequence":"additional","affiliation":[{"name":"Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention 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