{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T07:46:24Z","timestamp":1782805584593,"version":"3.54.5"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"vor","delay-in-days":179,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100008370","name":"National Pork Board","doi-asserted-by":"publisher","award":["21-134"],"award-info":[{"award-number":["21-134"]}],"id":[{"id":"10.13039\/100008370","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>African swine fever virus (ASFV) has recently been shown to be transmissible via artificial insemination (AI) with contaminated semen. In this study, we performed a comprehensive pathological investigation to assess vertical transmission and infection of the conceptus. A total of 14 gilts were experimentally inseminated and examined by clinical monitoring, virological analyses, gross and histopathology, immunohistochemistry, and combined RNAScope in situ hybridization and immunofluorescence to localize viral antigen and nucleic acids and to characterize infected cell populations. ASFV infection of conceptuses was demonstrated, supporting the possibility of vertical transmission. Viral antigen and nucleic acids were widely distributed across multiple tissues of the conceptus, indicating systemic infection. Infection patterns showed considerable heterogeneity, with variable viral load, morphology, and antigen detection observed both between animals and among conceptuses from the same uterus. Gross examination revealed uterine alterations, including hyperemia, edema, and accumulation of intrauterine fluid, frequently associated with embryonic loss or remnants of extraembryonic membranes. In the female reproductive tract, viral antigen was detected in multiple compartments, including the ovary, uterus, cervix, and vagina. Notably, viral antigen was detected in either maternal or fetal compartments but not simultaneously, indicating a temporally dynamic and spatially distinct pattern of infection and suggesting that multiple routes of maternal\u2010to\u2010conceptus transmission may be involved. Colocalization studies in fetal tissues revealed that ASFV\u2010infected cells included subsets expressing macrophage\u2010associated markers such as CD163 and Iba\u20101, while a substantial proportion of infected cells could not be phenotypically assigned, indicating a broader and incompletely defined cellular tropism. Together, these findings provide new insights into ASFV transmission biology and pathogenesis in early gestation and highlight the complexity of maternal\u2010to\u2010conceptus transmission, warranting further investigation of infection dynamics at the feto\u2010maternal interface and the identity of fetal target cell populations.<\/jats:p>","DOI":"10.1155\/tbed\/7164197","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T07:19:28Z","timestamp":1782803968000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Infection of the Conceptus With African Swine Fever Virus Following Artificial Insemination in Sows: A Pathological Study"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3884-1943","authenticated-orcid":false,"given":"Julia","family":"Sehl-Ewert","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5044-6508","authenticated-orcid":false,"given":"Virginia","family":"Friedrichs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5465-5609","authenticated-orcid":false,"given":"Sandra","family":"Blome","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,29]]},"reference":[{"key":"e_1_2_14_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2020.198099"},{"key":"e_1_2_14_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11121539"},{"key":"e_1_2_14_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/v14010031"},{"key":"e_1_2_14_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/v15030729"},{"key":"e_1_2_14_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2015.09.046"},{"key":"e_1_2_14_6_2","article-title":"Molecular Evidence of Transplacental (Vertical) Route of Transmission of African Swine Fever In Foetus of Pig: A Case Report","volume":"2","author":"Antiabong J.","year":"2006","journal-title":"Veterinary Medicine"},{"key":"e_1_2_14_7_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.1987.48.02.246"},{"key":"e_1_2_14_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/v14071387"},{"key":"e_1_2_14_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(00)00195-4"},{"key":"e_1_2_14_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-015-2430-2"},{"key":"e_1_2_14_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens13070537"},{"key":"e_1_2_14_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2012.12.018"},{"key":"e_1_2_14_13_2","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens11111386","article-title":"Pathology of African Swine Fever in Wild Boar Carcasses Naturally Infected With German Virus Variants","volume":"11","author":"Sehl-Ewert J.","year":"2022","journal-title":"Pathogens"},{"key":"e_1_2_14_14_2","doi-asserted-by":"crossref","DOI":"10.3390\/pathogens9080662","article-title":"Comparative Pathology of Domestic Pigs and Wild Boar Infected With the Moderately Virulent African Swine Fever Virus Strain \u201cEstonia 2014\u201d","volume":"9","author":"Sehl J.","year":"2020","journal-title":"Pathogens"},{"key":"e_1_2_14_15_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.1984.185.05.511"},{"key":"e_1_2_14_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-24740-1"},{"key":"e_1_2_14_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13567-025-01701-x"},{"key":"e_1_2_14_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/v17111472"},{"key":"e_1_2_14_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2022.110457"},{"key":"e_1_2_14_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-44-95"},{"key":"e_1_2_14_21_2","first-page":"233","article-title":"Evidence for the Transmission of Porcine Reproductive and Respiratory Syndrome (PRRS) Virus in Boar Semen","volume":"5","author":"Yaeger M. 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