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However, the widespread use of colistin has resulted in the rapid emergence of colistin resistance on a global scale. Ten members of the (mobile colistin resistance)\n                    <jats:italic>mcr<\/jats:italic>\n                    gene family,\n                    <jats:italic>mcr-1<\/jats:italic>\n                    through\n                    <jats:italic>mcr-10<\/jats:italic>\n                    , have been reported and documented. Currently, bacteria reported to carry the\n                    <jats:italic>mcr-10.1<\/jats:italic>\n                    gene are sensitive to colistin, but the mechanism underlying the low\u2010level resistance phenomenon mediated by\n                    <jats:italic>mcr-10.1<\/jats:italic>\n                    remains unclear.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    In this study, antimicrobial susceptibility testing (AST) was conducted on\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (E.coli) isolated from Chinese migratory birds, resulting in the selection of 87 strains exhibiting MDR phenotypes. Whole\u2010genome sequencing (draft) was performed on these 87 MDR\n                    <jats:italic>E. coli<\/jats:italic>\n                    strains, and for one of the\n                    <jats:italic>E. coli<\/jats:italic>\n                    strains carrying the\n                    <jats:italic>mcr-10.1<\/jats:italic>\n                    gene, whole\u2010genome sequencing, phenotypic characterization, AST and conjugation experiments were conducted to identify its resistance phenotypes and genetic characteristics.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    Whole\u2010genome sequencing (draft) of 87 MDR\n                    <jats:italic>E. coli<\/jats:italic>\n                    isolates revealed a diverse array of resistance genes, predominantly including aminoglycoside,\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010lactam, tetracycline, and sulfonamide resistance genes. Remarkably, one isolate, despite being sensitive to colistin, harbored the\n                    <jats:italic>mcr-10.1<\/jats:italic>\n                    gene. Further sequencing showed that\n                    <jats:italic>mcr-10.1<\/jats:italic>\n                    was located in the conserved region of\n                    <jats:italic>xerC-mcr-10.1<\/jats:italic>\n                    , a hotspot for movable elements with various insertion sequences (ISs) or transposons nearby. Phenotypic characterization indicated that the MDR plasmid pGN25\u2010\n                    <jats:italic>mcr10.1<\/jats:italic>\n                    had no significant effect on the growth of GN25 and its derivatives but reduced the number of bacterial flagella.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusions:<\/jats:bold>\n                    It is particularly important to note that bacteria harboring the\n                    <jats:italic>mcr-10.1<\/jats:italic>\n                    gene may exhibit low minimum inhibitory concentration (MIC) values, but that the MIC values under colistin selective pressure can become progressively higher and exacerbate the difficulty of treating infections caused by\n                    <jats:italic>mcr-10.1<\/jats:italic>\n                    \u2010associated bacteria. Therefore, vigilance for such \u201csilent transmission\u201d is warranted, and continuous monitoring of the spread of\n                    <jats:italic>mcr-10.1<\/jats:italic>\n                    is necessary in the future.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/7631217","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T08:32:54Z","timestamp":1746174774000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Integrated Genomic and Phenotypic Characterization of an\n                    <i>Mcr-10.1<\/i>\n                    \u2010Harboring Multidrug Resistant\n                    <i>Escherichia coli<\/i>\n                    Strain From Migratory Birds in China"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3417-3952","authenticated-orcid":false,"given":"Ronglei","family":"Huang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9754-8739","authenticated-orcid":false,"given":"Xue","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8607-6916","authenticated-orcid":false,"given":"Bing","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7702-2959","authenticated-orcid":false,"given":"Bowen","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-5779-8567","authenticated-orcid":false,"given":"Danhong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3266-4488","authenticated-orcid":false,"given":"Yi","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1110-1595","authenticated-orcid":false,"given":"Chengyang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6638-2478","authenticated-orcid":false,"given":"Ang","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2616-4619","authenticated-orcid":false,"given":"Nan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7485-084X","authenticated-orcid":false,"given":"Chongtao","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5336-5925","authenticated-orcid":false,"given":"Yang","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,2]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(15)00424-7"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkw245"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/22221751.2020.1732231"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms7100461"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-021-06307-y"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12061076"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0261444"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1017\/ash.2023.392"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00213-21"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab301"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr039"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky352"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2021.771510"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2022.114032"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2022.106645"},{"key":"e_1_2_12_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13756-022-01194-9"},{"key":"e_1_2_12_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2022.03.015"},{"key":"e_1_2_12_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2021.106418"},{"key":"e_1_2_12_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/mra.00165-22"},{"key":"e_1_2_12_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.00352"},{"key":"e_1_2_12_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2015.03.005"},{"key":"e_1_2_12_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkw173"},{"key":"e_1_2_12_23_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178598"},{"key":"e_1_2_12_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2018.01.004"},{"key":"e_1_2_12_25_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2021.0267"},{"key":"e_1_2_12_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.01127-22"},{"key":"e_1_2_12_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2020.115706"},{"key":"e_1_2_12_28_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.22538"},{"key":"e_1_2_12_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/ismejo\/wrae037"},{"key":"e_1_2_12_30_2","doi-asse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