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Their subtypes mainly included IgG1\/kappa, IgG2a\/kappa, IgG1\/lambda, IgG2b\/kappa, IgG2b\/lambda, and IgM\/kappa. Based on the mAb 8F9 with the highest blocking activity, a blocking enzyme\u2010linked immunosorbent assay (ELISA) method for detecting cat calicivirus antibodies was established. After systematic optimization, the antigen coating concentration was determined to be 1.0\u2009\u03bcg\/mL, the serum dilution was 1:8, and the dilution of horseradish peroxidase (HRP)\u2010labeled mAb was 1:400. The critical value for detecting cat serum was 23.5%, the sensitivity was 94.9%, and the specificity was 93.02%. There was no cross\u2010reaction with common pathogens such as feline parvovirus and feline mycoplasma. The repeatability was good, with intra\u2010batch and inter\u2010batch coefficients of variation (CV) &lt;10%. Clinical validation showed that the total coincidence rate with the indirect ELISA method recommended in the local standard was 96.19% (kappa\u2009=\u20090.9). The sensitive and specific blocking ELISA method established in this study can provide reliable technical support for FCV antibody screening, vaccine immune evaluation, and specific pathogen\u2010free (SPF) cat breeding.<\/jats:p>","DOI":"10.1155\/tbed\/7831655","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T06:49:10Z","timestamp":1782802150000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development and Application of a Blocking ELISA for the Detection of Feline Calicivirus Antibodies Based on Monoclonal Antibodies Against VP1 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