{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T07:31:47Z","timestamp":1786606307312,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:00:00Z","timestamp":1786579200000},"content-version":"vor","delay-in-days":224,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    is an important zoonotic opportunistic pathogen. Of particular concern is the emergence of multidrug\u2010resistant hypervirulent\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    (MDR\u2010hvKP), which represents a serious public health threat. Bacteriophage (phage) therapy has emerged as a promising solution to combat antibiotic\u2010resistant\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    infections. Here, we characterize a novel lytic phage, vB_Kp_H122, that specifically targets K57 capsular\u2010type MDR\u2010hvKP. vB_Kp_H122 exhibited the characteristic morphology of a siphovirus and demonstrated efficient infection kinetics, with an optimal multiplicity of infection (MOI) of 0.001 and a latent period of ~5\u2009min. It also demonstrated considerable stability across a pH range of 4\u201311 and at temperatures from 4\u00b0C to 50\u00b0C, as well as potent activity against\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    biofilms. The phage has a linear double\u2010stranded DNA genome of 46,077 bp with a G\u2009+\u2009C content of 47.61% and belongs to a novel species within the genus\n                    <jats:italic>Roufvirus<\/jats:italic>\n                    . Its genome contained no identifiable genes associated with lysogeny, virulence, or antibiotic resistance, supporting its therapeutic safety. In a mouse infection model, a single dose of vB_Kp_H122 at 2\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    PFU significantly reduced bacterial loads in organs, alleviated pathological damage, and provided complete protection against lethal challenge with K57 MDR\u2010hvKP. These findings suggest that vB_Kp_H122 may have potential as an antibacterial candidate against K57 MDR\u2010hvKP isolates.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/8429457","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T07:21:09Z","timestamp":1786605669000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization and Therapeutic Potential of a Novel Lytic Bacteriophage vB_Kp_H122 Targeting Multidrug\u2010Resistant Hypervirulent\n                    <i>Klebsiella pneumoniae<\/i>\n                    of K57 Capsular Type"],"prefix":"10.1155","volume":"2026","author":[{"given":"Cuilong","family":"Fan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiu","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Schwarz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaowei","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susu","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxuan","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longhua","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangyu","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2041-2208","authenticated-orcid":false,"given":"Fang","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9171-8637","authenticated-orcid":false,"given":"Wanjiang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fux013"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(24)00737-2"},{"key":"e_1_2_12_3_2","doi-asserted-by":"crossref","unstructured":"RussoT. A. AlvaradoC. L. andDaviesC. J. et al.Differentiation of Hypervirulent and ClassicalKlebsiella pneumoniaeWith Acquired Drug Resistance 2024 15 no. 2 https:\/\/doi.org\/10.1128\/mbio.02867-23.","DOI":"10.1128\/mbio.02867-23"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2024.105331"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.130917"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep15573"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2024.2439509"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.20411\/pai.v10i1.777"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1586\/eri.13.12"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008114"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/20477724.2022.2121362"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/fpd.2019.2657"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11259-024-10598-4"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2018.0456"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11010007"},{"key":"e_1_2_12_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.852596"},{"key":"e_1_2_12_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciad335"},{"key":"e_1_2_12_18_2","doi-asserted-by":"publisher","DOI":"10.2174\/0113816128343976241117183624"},{"key":"e_1_2_12_19_2","doi-asserted-by":"publisher","DOI":"10.1042\/EBC20240015"},{"key":"e_1_2_12_20_2","doi-asserted-by":"crossref","DOI":"10.1016\/j.foodcont.2024.110936","article-title":"A Novel K57-Specific Klebsiella pneumoniae Phage Disinfects Milk and Inhibits Biofilm Formation","volume":"168","author":"Xu S.","year":"2025","journal-title":"Food Control"},{"key":"e_1_2_12_21_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000800"},{"key":"e_1_2_12_22_2","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing","author":"Clinical and Laboratory Standards Institute","year":"2023"},{"key":"e_1_2_12_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2024.199473"},{"key":"e_1_2_12_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24044038"},{"key":"e_1_2_12_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2022.198750"},{"key":"e_1_2_12_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14071421"},{"key":"e_1_2_12_27_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2022.897531"},{"key":"e_1_2_12_28_2","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14091916"},{"key":"e_1_2_12_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.102"},{"key":"e_1_2_12_30_2","doi-asserted-by":"crossref","DOI":"10.1101\/pdb.prot5368","article-title":"Using the Metagenomics RAST Server (MG-RAST) for Analyzing Shotgun Metagenomes","volume":"2010","author":"Glass E. 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P.","year":"2020","journal-title":"Nucleic Acids Research"},{"key":"e_1_2_12_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbx081"},{"key":"e_1_2_12_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy096"},{"key":"e_1_2_12_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr039"},{"key":"e_1_2_12_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0825-4"},{"key":"e_1_2_12_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00034-21"},{"key":"e_1_2_12_41_2","doi-asserted-by":"crossref","DOI":"10.3390\/ph18050724","article-title":"Hypervirulent Klebsiella pneumoniae: Insights Into Virulence, Antibiotic Resistance, and Fight Strategies Against a Superbug","volume":"18","author":"Hetta H. F.","year":"2025","journal-title":"Pharmaceuticals"},{"key":"e_1_2_12_42_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.4c00681"},{"key":"e_1_2_12_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2025.128228"},{"key":"e_1_2_12_44_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02030117"},{"key":"e_1_2_12_45_2","doi-asserted-by":"publisher","DOI":"10.3390\/v17010083"},{"key":"e_1_2_12_46_2","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2025.2450462"},{"key":"e_1_2_12_47_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00051"},{"key":"e_1_2_12_48_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13568-025-01919-0"},{"key":"e_1_2_12_49_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12223-011-0039-8"},{"key":"e_1_2_12_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-37176-z"},{"key":"e_1_2_12_51_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics15010055"}],"container-title":["Transboundary and Emerging Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tbed\/8429457","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/tbed\/8429457","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tbed\/8429457","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T07:21:13Z","timestamp":1786605673000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/tbed\/8429457"}},"subtitle":[],"editor":[{"given":"Anusak","family":"Kerdsin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":51,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/tbed\/8429457"],"URL":"https:\/\/doi.org\/10.1155\/tbed\/8429457","archive":["Portico"],"relation":{},"ISSN":["1865-1674","1865-1682"],"issn-type":[{"value":"1865-1674","type":"print"},{"value":"1865-1682","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2026-01-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-20","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-13","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"8429457"}}