{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T16:25:49Z","timestamp":1775060749981,"version":"3.50.1"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T00:00:00Z","timestamp":1774569600000},"content-version":"vor","delay-in-days":85,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32473057"],"award-info":[{"award-number":["32473057"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2024YFD1800103"],"award-info":[{"award-number":["2024YFD1800103"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    The pig sucking louse\n                    <jats:italic>Haematopinus suis<\/jats:italic>\n                    is a major swine ectoparasite and vector of pathogens, yet its microbiome remains understudied. Here, we described the isolation of a previously unrecognized bacterial strain, P8\u20109\n                    <jats:sup>T<\/jats:sup>\n                    , from the intestinal tract of\n                    <jats:italic>H. suis<\/jats:italic>\n                    .\u2009A comprehensive polyphasic analysis, including phenotypic characterization, phylogenomics, and whole\u2010genome comparisons, placed this isolate within\n                    <jats:italic>Peribacillus<\/jats:italic>\n                    . Genome\u2010based metrics confirmed its novelty, with average nucleotide identity and digital DNA\u2013DNA hybridization values (&lt;80% and &lt;27%, respectively) falling far below accepted species delineation thresholds. We, therefore, proposed the designation\n                    <jats:italic>Peribacillus suis<\/jats:italic>\n                    sp. nov. Unexpectedly, the genome of P8\u20109\n                    <jats:sup>T<\/jats:sup>\n                    encodes an extensive repertoire of 219 putative virulence\u2010associated and antibiotic resistance genes; features atypical for a largely composed of environmental species considered saprophytic or beneficial. In vivo experiments revealed this pathogenic potential: intraperitoneal inoculation in mice resulted in all animals reaching predefined humane endpoints within 24\u2009h, characterized by septicemia and widespread organ pathology, while oral exposure elicited splenomegaly, intestinal pathology, and a robust pro\u2010inflammatory response. This work represents the first report of a\n                    <jats:italic>Peribacillus<\/jats:italic>\n                    species isolated from an ectoparasite and provides direct experimental evidence of virulence within a genus traditionally viewed as benign.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/8640992","type":"journal-article","created":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T17:01:32Z","timestamp":1774630892000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Peribacillus suis<\/i>\n                    sp. nov. Isolated From the Pig Louse\n                    <i>Haematopinus suis<\/i>\n                    Reveals Unexpected Pathogenic Potential in a Traditionally Benign Genus"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-4352-0094","authenticated-orcid":false,"given":"Yan-Yan","family":"Peng","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3303-930X","authenticated-orcid":false,"given":"Hany M.","family":"Elsheikha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8301-5607","authenticated-orcid":false,"given":"Ying","family":"Xun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3008-3942","authenticated-orcid":false,"given":"Yi-Liu","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4758-5309","authenticated-orcid":false,"given":"Ya","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8529-5423","authenticated-orcid":false,"given":"Yuan-Ping","family":"Deng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3408-9158","authenticated-orcid":false,"given":"Shi-Feng","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8678-8796","authenticated-orcid":false,"given":"Yi-Tian","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8434-899X","authenticated-orcid":false,"given":"Guo-Hua","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,3,27]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-018-0435-2"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-025-02119-z"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-025-06964-z"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-023-00901-6"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens12030472"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2019.04.007"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2024.107106"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.003775"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.896728"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jambio\/lxae066"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e39973"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2025.144644"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens11101203"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/acmi.0.000911.v3"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro732"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2009.05.051"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906412106"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-60"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00492-13"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.01294"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002843"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.12.2607"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab688"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm160"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-017-0844-4"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab902"},{"key":"e_1_2_10_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit399"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1101\/pdb.prot5439"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.059774-0"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-018-0032-z"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00059-12"},{"key":"e_1_2_10_33_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00173"},{"key":"e_1_2_10_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/mec.12421"},{"key":"e_1_2_10_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2653"},{"key":"e_1_2_10_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2021.10.012"},{"key":"e_1_2_10_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-023-35356-5"},{"key":"e_1_2_10_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01925-24"}],"container-title":["Transboundary and Emerging Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tbed\/8640992","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/tbed\/8640992","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tbed\/8640992","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T14:47:20Z","timestamp":1775054840000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/tbed\/8640992"}},"subtitle":[],"editor":[{"given":"Zongfu","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/tbed\/8640992"],"URL":"https:\/\/doi.org\/10.1155\/tbed\/8640992","archive":["Portico"],"relation":{},"ISSN":["1865-1674","1865-1682"],"issn-type":[{"value":"1865-1674","type":"print"},{"value":"1865-1682","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-11-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"8640992"}}