{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T16:11:40Z","timestamp":1788797500977,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T00:00:00Z","timestamp":1788739200000},"content-version":"vor","delay-in-days":249,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018927","name":"Southwest Minzu University","doi-asserted-by":"publisher","award":["ZYN2026036"],"award-info":[{"award-number":["ZYN2026036"]}],"id":[{"id":"10.13039\/501100018927","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012542","name":"Sichuan Province Science and Technology Support Program","doi-asserted-by":"publisher","award":["2025ZNSFSC0192"],"award-info":[{"award-number":["2025ZNSFSC0192"]}],"id":[{"id":"10.13039\/100012542","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Porcine teschovirus (PTV) is the causative agent of porcine diarrhea and multisystem disorder and poses a global threat to the health of domestic pigs. However, its epidemic and pathogenic characteristics in Tibetan pigs, which are a unique indigenous breed in the Qinghai\u2013Tibet Plateau of China, remain largely unexplored. Here, we conducted a comprehensive investigation during 2024\u20132025, in which 303 diarrheic fecal samples were collected from 21 farms across eight counties in Ganzi Tibetan Autonomous Prefecture, with an average altitude of 3433\u2009m. RT\u2010PCR testing identified 141 PTV\u2010positive samples, yielding a high positivity rate of 46.5% (141\/303). From these positives, 27 VP1 sequences were cloned and sequenced; phylogenetic analysis revealed that 16 strains belonged to\n                    <jats:italic>Teschovirus A<\/jats:italic>\n                    , three strains were divided into\n                    <jats:italic>Teschovirus B<\/jats:italic>\n                    , and three strains formed the interspecies recombinant genotypes (PTV\u201015\/16). Interestingly, five novel strains were classified as undefined genotypes, indicating an extensive genetic diversity among PTV strains circulating in Tibetan pigs. Furthermore, a PTV strain, designated PTV\u2010SCgz\u201001, was successfully isolated in PK\u201015 cells, with a near\u2010complete genomic sequence of 7081 nucleotides. Phylogenetic analysis based on the polyprotein and the VP1 genes indicated that it belonged to genotype PTV\u20104, whereas recombination analysis revealed that PTV\u2010SCgz\u201001 is a natural recombinant with parental strains derived from HNMY (PTV\u20104) and China\/SWU\u2010ZG2\/2018 (PTV\u20106). Experimental infection of 17\u2010day\u2010old Tibetan piglets demonstrated that this isolate induces severe watery diarrhea. Notably, the virus also caused severe pulmonary hemorrhage and mild cerebral hyperemia with neuronal degeneration, and it had a high mortality rate (40%), suggesting that strain PTV\u2010SCgz\u201001 has strong pathogenic potential for 17\u2010day\u2010old Tibetan piglets. Our findings provide a more comprehensive molecular epidemiology of PTVs in Tibetan pigs and underscore the need for viral surveillance and control in this unique pig population.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/8803438","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T15:54:58Z","timestamp":1788796498000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Epidemiology and Pathogenicity Evaluation of Porcine Teschovirus in Tibetan Pigs on the Qinghai\u2013Tibet Plateau of China"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4710-0477","authenticated-orcid":false,"given":"Haohao","family":"Lu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danjiao","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Feng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwen","family":"Pei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhidong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2369-6058","authenticated-orcid":false,"given":"Long","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1177\/1040638712445769"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-012-1315-x"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063871102300231"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.05.001"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007050050709"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.000911"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.4.1620-1631.2001"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-015-2744-0"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2011.09.014"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-012-1327-6"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-019-02114-7"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2025.110398"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-014-2326-6"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-020-04525-6"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.1155\/2023\/9977581"},{"key":"e_1_2_12_16_2","doi-asserted-by":"publisher","DOI":"10.24425\/pjvs.2025.154950"},{"key":"e_1_2_12_17_2","first-page":"7","article-title":"Isolation and Molecular Characterization of a Porcine Teschovirus 1 Isolate From China","volume":"51","author":"Feng L.","year":"2007","journal-title":"Acta Virologica"},{"key":"e_1_2_12_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2010.03.019"},{"key":"e_1_2_12_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2013.06.005"},{"key":"e_1_2_12_20_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.001129"},{"key":"e_1_2_12_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-022-03549-1"},{"key":"e_1_2_12_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/v17091200"},{"key":"e_1_2_12_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2026.110989"},{"key":"e_1_2_12_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2012.04.025"},{"key":"e_1_2_12_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2811"},{"key":"e_1_2_12_26_2","first-page":"185","article-title":"Detection and Genetic Evolution of Teschovirus of Tibetan Pig Origin in 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