{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:43:48Z","timestamp":1773009828828,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,8,8]],"date-time":"2025-08-08T00:00:00Z","timestamp":1754611200000},"content-version":"vor","delay-in-days":219,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32470195"],"award-info":[{"award-number":["32470195"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31870121"],"award-info":[{"award-number":["31870121"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Hydroxychloroquine (HCQ) has showed beneficial effects on pregnancy outcomes in systemic erythaematosus lupus (SLE) by its anti\u2010inflammatory and immunomodulatory properties. However, the role of HCQ in preventing abortion caused by infectious diseases remains unknown. In this study, we choose\n                    <jats:italic>B. abortus<\/jats:italic>\n                    \u2010induced abortion of pregnant mice as a model to investigate the effect of HCQ in preventing abortion. HCQ decreases the pH value of endosomal\n                    <jats:italic>Brucella<\/jats:italic>\n                    \u2010containing vacuole (eBCV),\n                    <jats:italic>B. abortus<\/jats:italic>\n                    type IV secretion system (T4SS) expression in trophoblasts and\n                    <jats:italic>Brucella<\/jats:italic>\n                    intracellular growth and cell death. Moreover, administration of HCQ decreases inflammation triggered by dead trophoblasts in a T4SS\u2010independent manner. Mechanistically, this suppressed inflammatory response is due to inhibition of both NF\u2010kB and mitogen\u2010activated protein kinases (MAPKs) activation by HCQ. In the murine model of abortion, HCQ treatment enhanced pup viability through suppressing\n                    <jats:italic>B. abortus<\/jats:italic>\n                    growth within placenta and preventing placentitis. Our results suggest administration of HCQ could be an effective protection against abortion induced by\n                    <jats:italic>Brucella<\/jats:italic>\n                    infection, which imply that HCQ may be beneficial in treating abortion caused by infectious diseases.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/8853857","type":"journal-article","created":{"date-parts":[[2025,8,9]],"date-time":"2025-08-09T06:34:33Z","timestamp":1754721273000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effect of Hydroxychloroquine in a Mouse Model of Abortion Caused by\n                    <i>Brucella abortus<\/i>\n                    Infection"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1172-5458","authenticated-orcid":false,"given":"Haoran","family":"Liu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9479-4246","authenticated-orcid":false,"given":"Zhirong","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-9238-039X","authenticated-orcid":false,"given":"Jingyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3487-5510","authenticated-orcid":false,"given":"Xiaofeng","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4070-2378","authenticated-orcid":false,"given":"Weiyu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6597-586X","authenticated-orcid":false,"given":"Jun","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6971-6774","authenticated-orcid":false,"given":"Qisheng","family":"Peng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,8]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00062-23"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/aro2.70001"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcpa.2008.10.004"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.01088"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/193047a0"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2016.08.007"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.13080"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiaa722"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.00013-22"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature17631"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.BAI-0006-2019"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-5-22"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01538-19"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/aro2.5"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/bcp.14131"},{"key":"e_1_2_12_16_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01409"},{"key":"e_1_2_12_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40261-018-0656-y"},{"key":"e_1_2_12_18_2","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/4582612"},{"key":"e_1_2_12_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0176457"},{"key":"e_1_2_12_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-020-0372-x"},{"key":"e_1_2_12_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-54556-6"},{"key":"e_1_2_12_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032514299"},{"key":"e_1_2_12_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01730-16"},{"key":"e_1_2_12_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.8.4041-4047.1999"},{"key":"e_1_2_12_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00718.x"},{"key":"e_1_2_12_26_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030088"},{"key":"e_1_2_12_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/15548627.2024.2362122"},{"key":"e_1_2_12_28_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.70.681"},{"key":"e_1_2_12_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1863"}],"container-title":["Transboundary 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