{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T12:05:20Z","timestamp":1779883520997,"version":"3.53.1"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":146,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["KY201901014"],"award-info":[{"award-number":["KY201901014"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Liaoning Province possesses distinct ecological characteristics, including high forest cover and developed agriculture, which provide suitable habitats for various rodent species. Over the years, hemorrhagic fever with renal syndrome (HFRS), caused by members of the genus\n                      <jats:italic>Orthohantavirus<\/jats:italic>\n                      , such as\n                      <jats:italic>Orthohantavirus hantanense<\/jats:italic>\n                      (HTNV) and\n                      <jats:italic>Orthohantavirus seoulense<\/jats:italic>\n                      , remains highly prevalent in Liaoning Province, representing a significant public health concern. The transmission of these viruses is sustained by wild rodent reservoirs, necessitating continuous surveillance of infection in these host populations. This study is designed to investigate the distribution and genetic evolution of\n                      <jats:italic>Orthohantavirus<\/jats:italic>\n                      infections within wild rodent reservoir populations in Liaoning Province from 2022 to 2023 and to provide a scientific basis for effective prevention and control strategies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We conducted an epidemiological investigation of 403 wild rodents collected across Liaoning Province. Samples were screened using a combination of metatranscriptomic next\u2010generation sequencing (mNGS) and specific two\u2010step RT\u2010PCR protocols.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We identified a total of 19\n                      <jats:italic>Orthohantavirus<\/jats:italic>\n                      \u2010positive rodent samples (4.71% prevalence). Initial screening based on national standards (SN\/T 2777\u20102011) identified 15 positive samples, primarily from the Fushun, Shenyang, and Chaoyang areas. Notably, while standard RT\u2010PCR failed to detect viral RNA in certain samples from the Dandong area, mNGS revealed HTNV nucleic acid sequences, indicating significant genomic divergence in the primer\u2010binding regions of the M segment. Consequently, based on the obtained M\u2010segment sequences, we designed novel, strain\u2010specific primers (HTN\u2010DD), which successfully identified an additional four HTNV\u2010positive samples in the Dandong area. Sequence alignment demonstrated high sequence identity with major strains found in local human infections among these rodent\u2010derived sequences.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>HTNV and SEOV exhibit a significant prevalence in wild rodents across Liaoning, which confirms the region remains a high\u2010risk area for HFRS. Our findings suggest that viral genomic divergence can compromise standard molecular detection. Therefore, surveillance protocols must be updated to avoid false negatives in clinical diagnosis and epidemiological investigations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/tbed\/8897783","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T11:06:14Z","timestamp":1779879974000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Epidemiological Investigation and Analysis of\n                    <i>Orthohantavirus hantanense<\/i>\n                    and\n                    <i>Orthohantavirus seoulense<\/i>\n                    From Wild Rodents in Liaoning Province, China"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-9882-6797","authenticated-orcid":false,"given":"Yijia","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7429-5483","authenticated-orcid":false,"given":"Qiqi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2749-9756","authenticated-orcid":false,"given":"Qing","family":"Xin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7862-783X","authenticated-orcid":false,"given":"Mingxuan","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongxiang","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zimeng","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaying","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxing","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9910-8710","authenticated-orcid":false,"given":"Xiaohu","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6059-9689","authenticated-orcid":false,"given":"Feng","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-1027-8690","authenticated-orcid":false,"given":"Hongwei","family":"Xin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,27]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.3390\/diseases11010038"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12920-024-02004-4"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-416975-3.00003-0"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2016.00001"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2013.12.036"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2023.156340"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/v13081561"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-005-0555-4"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-77-11-2677"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0302.970202"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1512.090229"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2310.170842"},{"key":"e_1_2_12_13_2","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000039737"},{"key":"e_1_2_12_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/v16010145"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000789"},{"key":"e_1_2_12_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006198"},{"key":"e_1_2_12_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0012151"},{"key":"e_1_2_12_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006901"},{"key":"e_1_2_12_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0012478"},{"key":"e_1_2_12_20_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1608.090470"},{"key":"e_1_2_12_21_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.748232"},{"key":"e_1_2_12_22_2","unstructured":"Standardization 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