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This study aimed to characterize isolated tigecycline\u2010resistant strains from T\u00fcrkiye by using whole\u2010genome sequencing (WGS). The genomes of 40 strains, recovered from turkey caecum samples (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200923,\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    ), chicken caecum samples (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20098,\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    ;\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20092,\n                    <jats:italic>K. oxytoca<\/jats:italic>\n                    ), and chicken meat samples (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20097,\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    ), were determined. Additionally, Oxford Nanopore Sequencing was conducted on subsets (\n                    <jats:italic>mcr<\/jats:italic>\n                    \u20108\u2010positive,\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20099) to characterize the plasmids responsible for colistin resistance spread. Sequence analysis revealed that all\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    harbored the\n                    <jats:italic>tet<\/jats:italic>\n                    (A) gene without detectable mutations, whereas several alterations were detected in the\n                    <jats:italic>ramR<\/jats:italic>\n                    gene. Additionally, other tetracycline\u2010encoding genes were also detected in few isolates, including\n                    <jats:italic>tet<\/jats:italic>\n                    (B),\n                    <jats:italic>tet<\/jats:italic>\n                    (D),\n                    <jats:italic>tet<\/jats:italic>\n                    (J), and\n                    <jats:italic>tet<\/jats:italic>\n                    (M). Genomic analysis revealed various AMR genes, including the plasmid\u2010mediated colistin resistance gene\n                    <jats:italic>mcr<\/jats:italic>\n                    \u20108, for which hybrid assemblies confirmed the localization on IncF group plasmids associated with IS\n                    <jats:italic>903B<\/jats:italic>\n                    . Conjugation assay showed the transferability of\n                    <jats:italic>mcr<\/jats:italic>\n                    \u20108 in isolates (8\/9) to\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    recipient strains. WGS analysis also revealed 11 different sequence types (STs), with ST37 appearing as the predominant lineage among both tigecycline\u2010 and colistin\u2010resistant isolates. Phylogenetic comparison with previously sequenced isolates showed close genetic relatedness between certain isolates and international clones. To our knowledge, this is the first report of\n                    <jats:italic>mcr<\/jats:italic>\n                    \u20108 in\n                    <jats:italic>Klebsiella<\/jats:italic>\n                    strains from food animals in T\u00fcrkiye, highlighting a potential risk for transmission of last\u2010resort antimicrobial resistance genes to humans. Hence, continuous genomic surveillance is urgently needed to monitor the spread of these resistance determinants within the One Health perspective.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/9122655","type":"journal-article","created":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T06:32:58Z","timestamp":1776753178000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Whole\u2010Genome Insights Into Tigecycline\u2010Resistant\n                    <i>Klebsiella<\/i>\n                    Spp. Harboring Colistin Resistance Gene From Food Animals in 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A.","family":"Hammerl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8333-9891","authenticated-orcid":false,"given":"Ruichao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,20]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1139\/cjm-2024-0032"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsre\/fux013"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2018.09.012"},{"key":"e_1_2_12_4_2","unstructured":"World Health Organization World Health Organization Bacterial Priority Pathogens List 2024: Bacterial Pathogens of Public Health Importance to Guide Research Development and Strategies to Prevent and Control Antimicrobial Resistance 2024 WHO accessed 16 July 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