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Here, we first report the cocarrying\n                    <jats:italic>poxtA<\/jats:italic>\n                    (oxazolidinone resistance) and\n                    <jats:italic>pbp5fm<\/jats:italic>\n                    (\u03b2\u2010lactam resistance) genes by the plasmid in a strain of\n                    <jats:italic>Enterococcus hirae<\/jats:italic>\n                    HDC14\u20102 derived from porcine. The isolate also exhibits MDR phenotypes to phenicols, oxazolidinones, tetracyclines, \u03b2\u2010lactams, aminoglycosides, macrolides, and lincosamides. Whole\u2010genome sequencing (WGS) revealed these resistance genes, along with\n                    <jats:italic>tet<\/jats:italic>\n                    (\n                    <jats:italic>L<\/jats:italic>\n                    ),\n                    <jats:italic>tet<\/jats:italic>\n                    (\n                    <jats:italic>M<\/jats:italic>\n                    ),\n                    <jats:italic>catA<\/jats:italic>\n                    ,\n                    <jats:italic>erm<\/jats:italic>\n                    (B),\n                    <jats:italic>aac<\/jats:italic>\n                    (\n                    <jats:italic>6<\/jats:italic>\n                    )\u2010\n                    <jats:italic>aph<\/jats:italic>\n                    (\n                    <jats:italic>2\u201d<\/jats:italic>\n                    ),\n                    <jats:italic>aadE<\/jats:italic>\n                    ,\n                    <jats:italic>spw<\/jats:italic>\n                    ,\n                    <jats:italic>lsa<\/jats:italic>\n                    (E),\n                    <jats:italic>lnu<\/jats:italic>\n                    (B),\n                    <jats:italic>sat4<\/jats:italic>\n                    , and\n                    <jats:italic>aphA3<\/jats:italic>\n                    , were clustered in a novel MDR region flanked by IS\n                    <jats:italic>1216<\/jats:italic>\n                    elements on plasmid pHDC14\u20102.133K. This IS\n                    <jats:italic>1216<\/jats:italic>\n                    \u2010bounded MDR region formed translocatable units (TUs), including an IS\n                    <jats:italic>1216<\/jats:italic>\n                    \u2010\n                    <jats:italic>poxtA<\/jats:italic>\n                    TU that was also identified on a secondary plasmid, pHDC14\u20102.27K. Functional assays demonstrated the excisability and mobility of these TUs, indicating its potential ability integration into other plasmids or chromosomes. Critically, electrotransformation confirmed the transfer of pHDC14\u20102.27K (\n                    <jats:italic>poxtA<\/jats:italic>\n                    \u2010carrying) to\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    JH2\u20102, with retained TU activity and minimal fitness cost. This study provides the evidence of colocalized\n                    <jats:italic>poxtA<\/jats:italic>\n                    and\n                    <jats:italic>pbp5fm<\/jats:italic>\n                    on plasmids in enterococci, highlighting their role in disseminating pan\u2010resistance among bacteria. Although\n                    <jats:italic>E. hirae<\/jats:italic>\n                    is not an important pathogenic bacterium to humans and animals, but its potential risk to horizontally spread of these resistance genes important in medicine still cannot be ignored.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/9275403","type":"journal-article","created":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T04:35:07Z","timestamp":1752467707000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Genetic Characterization of a Linezolid\u2010 and Penicillin\u2010Resistant\n                    <i>Enterococcus hirae<\/i>\n                    Isolate Co\u2010Harboring\n                    <i>poxtA<\/i>\n                    and\n                    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