{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:41:31Z","timestamp":1792608091984,"version":"4.5.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"vor","delay-in-days":293,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Human babesiosis, an emerging zoonosis with ongoing geographic expansion, is primarily caused by\n                    <jats:italic>Babesia microti<\/jats:italic>\n                    , a tick\u2010borne apicomplexan parasite that resides within host erythrocytes. While small mammals serve as its recognized reservoir hosts, the relative contribution of sympatric wild rodents and shrews in subtropical mountain endemic areas of Eastern China remains poorly characterized. In South\u2010central Zhejiang, an endemic focus of human babesiosis, systematic data on local small\u2010mammal hosts remain limited. In this study, 630 small mammals, comprising six rodent species and the shrew\n                    <jats:italic>Suncus murinus<\/jats:italic>\n                    , were captured across six counties in Zhejiang Province of China. Host identity was confirmed by morphological examination and mitochondrial cytochrome b (\n                    <jats:italic>cytb<\/jats:italic>\n                    ) barcoding. Whole\u2010blood DNA was screened for\n                    <jats:italic>B<\/jats:italic>\n                    .\n                    <jats:italic>microti<\/jats:italic>\n                    by TaqMan real\u2010time quantitative PCR (qPCR); positive specimens were further analyzed by nested PCR (NPCR) targeting the\n                    <jats:italic>18S rRNA<\/jats:italic>\n                    gene, followed by sequencing for genotype identification. The overall qPCR\u2010positive rate was 3.0% (19\/630). Geographically,\n                    <jats:italic>B. microti<\/jats:italic>\n                    infections were detected in four out of six surveyed counties, with Jinyun County showing the highest prevalence of 6.9%, followed by Qingyuan (4.0%), Wencheng (3.2%), and Qingtian (3.0%); no positive samples were identified in the Yunhe and Jingning counties. Among the sampled species,\n                    <jats:italic>Niviventer lotipes<\/jats:italic>\n                    showed the highest prevalence (11.7%, 12\/103), followed by\n                    <jats:italic>S. murinus<\/jats:italic>\n                    (3.8%, 2\/52),\n                    <jats:italic>Alexandromys fortis<\/jats:italic>\n                    (3.7%, 3\/81),\n                    <jats:italic>Apodemus agrarius<\/jats:italic>\n                    (1.0%, 1\/99), and\n                    <jats:italic>Rattus norvegicus<\/jats:italic>\n                    (0.5%, 1\/222). No positive results were detected in\n                    <jats:italic>R. tanezumi<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200962) or\n                    <jats:italic>Leopoldamys edwardsi<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200911). Fifteen of the 19 samples yielded positive amplification by NPCR and were successfully sequenced. All 15 sequenced isolates were assigned to the Kobe genotype and exhibited close genetic relatedness to previously reported local human\u2010derived and tick\u2010derived strains. The dominance of a single haplotype (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u200914) across host species and counties indicates genetically homogeneous circulation of the Kobe genotype within local small\u2010mammal communities, with host species rather than sampling county as the significant determinant of infection risk. These findings suggest that\n                    <jats:italic>N. lotipes<\/jats:italic>\n                    may be an important reservoir host in this region, and shrews may represent understudied secondary hosts, supporting the inclusion of both rodents and shrews in regional babesiosis surveillance.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/9501371","type":"journal-article","created":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:17:07Z","timestamp":1792606627000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Prevalence and Genotype Detection of\n                    <i>Babesia microti<\/i>\n                    in Multiple Wild Rodent Species and\n                    <i>Suncus murinus<\/i>\n                    From Subtropical Mountainous Regions of South\u2010Central Zhejiang, China"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1088-9807","authenticated-orcid":false,"given":"Jie","family":"Xu","sequence":"first","affiliation":[{"name":"School of Public Health Hangzhou Medical College  Hangzhou Zhejiang China  hznu.edu.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4833-7329","authenticated-orcid":false,"given":"Jia-Qi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou Zhejiang China  cdc.zj.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4288-5516","authenticated-orcid":false,"given":"Jian-Feng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou Zhejiang China  cdc.zj.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6681-0266","authenticated-orcid":false,"given":"Yan","family":"Feng","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou Zhejiang China  cdc.zj.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3987-7509","authenticated-orcid":false,"given":"Xuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou Zhejiang China  cdc.zj.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7430-9575","authenticated-orcid":false,"given":"Wen-Jie","family":"Xu","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou Zhejiang China  cdc.zj.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5291-0909","authenticated-orcid":false,"given":"Xiao-Xiao","family":"Wang","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou Zhejiang China  cdc.zj.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7354-3450","authenticated-orcid":false,"given":"Ji-Min","family":"Sun","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou Zhejiang China  cdc.zj.cn"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3717-3055","authenticated-orcid":false,"given":"Wei","family":"Ruan","sequence":"additional","affiliation":[{"name":"Zhejiang Provincial Center for Disease Control and Prevention  Hangzhou Zhejiang China  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