{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T06:41:26Z","timestamp":1776840086504,"version":"3.51.2"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,4,21]],"date-time":"2026-04-21T00:00:00Z","timestamp":1776729600000},"content-version":"vor","delay-in-days":110,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32273015"],"award-info":[{"award-number":["32273015"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Porcine epidemic diarrhea (PED) is the severe infectious disease caused by the porcine epidemic diarrhea virus (PEDV), characterized by the onset of watery diarrhea.\n                    <jats:italic>Anemarrhena asphodeloides<\/jats:italic>\n                    polysaccharide, extracted from\n                    <jats:italic>Anemarrhena asphodeloides<\/jats:italic>\n                    , presents potential applications as immunostimulatory and anticancer agent in alternative therapies. However, its antiviral activity remains unreported. This study investigates the inhibitory effects of\n                    <jats:italic>Anemarrhena asphodeloides<\/jats:italic>\n                    polysaccharide on PEDV infection. The findings demonstrate, for the first time, that\n                    <jats:italic>Anemarrhena asphodeloides<\/jats:italic>\n                    polysaccharide can effectively inhibit PEDV infection in a dose\u2010dependent manner. It does not impact PEDV release and deactivation. Through the network pharmacology and bioinformatics analysis, we focused on the antiviral mechanism of\n                    <jats:italic>Anemarrhena asphodeloides<\/jats:italic>\n                    polysaccharides in terms of apoptosis and oxidative stress. We revealed that\n                    <jats:italic>Anemarrhena asphodeloides<\/jats:italic>\n                    polysaccharide significantly decreases oxidative stress and caspase\u20103 dependent apoptosis in infected cells. Apoptosis inhibitor Ac\u2010DEVD\u2010CHO and ROS scavenger NAC disrupt PEDV infection, and NAC reduces caspase\u20103 dependent apoptosis. This study provides preliminary evidence of the anti\u2010PEDV activity of\n                    <jats:italic>Anemarrhena asphodeloides<\/jats:italic>\n                    polysaccharide by targeting ROS\/caspase\u20103 dependent apoptosis, establishing it as novel anti\u2010PEDV agent.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/9703618","type":"journal-article","created":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T05:44:30Z","timestamp":1776836670000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Anemarrhena asphodeloides<\/i>\n                    Polysaccharide Exerts Antiviral Activity Against Porcine Epidemic Diarrhea Virus Targeting ROS\/Caspase\u20103 Dependent Apoptosis"],"prefix":"10.1155","volume":"2026","author":[{"given":"Long","family":"Yin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaotong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianbin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7341-690X","authenticated-orcid":false,"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingting","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luyao","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4814-2353","authenticated-orcid":false,"given":"Song","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9904-8893","authenticated-orcid":false,"given":"Changchao","family":"Huan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,21]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13530-016-0287-8"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-012-0713-1"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.13439"},{"key":"e_1_2_8_4_2","doi-asserted-by":"crossref","first-page":"1350","DOI":"10.3201\/eid1803.120002","article-title":"New 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