{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T15:13:35Z","timestamp":1786115615071,"version":"build-2736575974"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":216,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["192763"],"award-info":[{"award-number":["192763"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009068","name":"Universit\u00e4t Bern","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009068","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Antimicrobial resistance (AMR) in Enterobacterales, particularly extended\u2010spectrum beta\u2010lactamase\u2010producing\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (ESBL\u2010Ec), poses a significant public health concern. Widespread antimicrobial use exerts selection pressure, driving the persistence and spread of resistant bacteria in diverse environments. While ESBL\u2010Ec is well\u2010documented in clinical and agricultural settings, its presence in animal reservoirs remains poorly understood. This study assessed the prevalence, AMR profiles, and genetic diversity of ESBL\u2010Ec in Swiss dairy cattle and wildlife. Between 2021 and 2023, 775 samples were analyzed, including rectal swab samples from dairy cows (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009475) and fecal or cloacal swab samples from wildlife (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u2009300). ESBL\u2010Ec was detected in 3\/475 (0.6%, 95% confidence interval [CI] 0.2%\u20131.8%) of dairy cattle and 15\/300 (5%, 95% CI 3.1%\u20138.1%) wildlife samples, with higher prevalence in birds (6\/99, 6.1%, 95% CI 2.8%\u201312.6%) than mammals (9\/200, 4.5%, 95% CI 2.4%\u20138.3%). Whole\u2010genome sequencing (WGS) of 54 ESBL\u2010Ec isolates identified 28 sequence types (STs) with no overlap between livestock and wildlife, suggesting distinct evolutionary trajectories. Phenotypic testing revealed resistance beyond beta\u2010lactams, notably against aminoglycosides, tetracyclines, and sulfonamides, whereas all isolates remained susceptible to tigecycline and meropenem. Multidrug resistance (MDR) was prevalent (89%), with bla\n                    <jats:sub>TEM-1<\/jats:sub>\n                    (in dairy cattle), bla\n                    <jats:sub>CTX-M55<\/jats:sub>\n                    and bla\n                    <jats:sub>TEM-176<\/jats:sub>\n                    (in red foxes) widely distributed across Switzerland. Clonal plasmids (IncFIB, IncX1) were detected in both wildlife and dairy cattle, and some animals harbored multiple ESBL\u2010Ec strains. Despite the low prevalence of plasmids, spatial clustering indicated local persistence in both livestock and wildlife, highlighting potential transmission. The observed presence of clinically relevant ESBL\u2010Ec in wildlife highlights the need for a One Health approach to tackle the dangers of AMR. Our findings contribute to AMR surveillance by providing baseline data on ESBL\u2010Ec animal reservoirs and informing the design of strategies to mitigate its environmental spread.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/9907660","type":"journal-article","created":{"date-parts":[[2026,8,6]],"date-time":"2026-08-06T03:58:01Z","timestamp":1785988681000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Extended\u2010Spectrum Beta\u2010Lactamase (ESBL)\u2010Producing\n                    <i>E. coli<\/i>\n                    in Livestock and Free\u2010Roaming Wildlife: A Combined Phenotyping\u2014Whole\u2010Genome Sequencing One Health Approach"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0173-6170","authenticated-orcid":false,"given":"Sheena","family":"Conforti","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4192-4923","authenticated-orcid":false,"given":"Adamandia","family":"Kapopoulou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4730-758X","authenticated-orcid":false,"given":"Claudia","family":"Bank","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0068-9076","authenticated-orcid":false,"given":"Belinda","family":"K\u00f6chle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1888-9226","authenticated-orcid":false,"given":"Bahtiyar","family":"Yilmaz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2594-007X","authenticated-orcid":false,"given":"Jens","family":"Becker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2017.01.013"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkz199"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2022.27.49.2200343"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071725"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/mbo3.845"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2023.122476"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2023.106342"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1089\/mdr.2019.0477"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2023.168625"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1079\/PAVSNNR202015010"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.626774"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9100678"},{"key":"e_1_2_11_13_2","unstructured":"Federal Office of Public HealthandFederal Food Safety and Veterinary Office Usage of Antibiotics and Occurrence of Antibiotic Resistance in Switzerland 2024 Swiss Antibiotic Resistance Reporthttps:\/\/www.anresis.ch\/wp-content\/uploads\/2024\/11\/SARR24.pdf."},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0310431"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envres.2025.121968"},{"key":"e_1_2_11_16_2","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing","author":"Lewis J. 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