{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:40:44Z","timestamp":1791517244119,"version":"4.3.4"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T00:00:00Z","timestamp":1762819200000},"content-version":"vor","delay-in-days":314,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006247","name":"Anhui University of Science and Technology","doi-asserted-by":"publisher","award":["XK-XJGF002"],"award-info":[{"award-number":["XK-XJGF002"]}],"id":[{"id":"10.13039\/501100006247","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006247","name":"Anhui University of Science and Technology","doi-asserted-by":"publisher","award":["DKYJ202205"],"award-info":[{"award-number":["DKYJ202205"]}],"id":[{"id":"10.13039\/501100006247","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32302854"],"award-info":[{"award-number":["32302854"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Transboundary and Emerging Diseases"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:italic>Pasteurella multocida<\/jats:italic>\n                    (\n                    <jats:italic>P. multocida<\/jats:italic>\n                    ), a significant animal pathogen, causes swine pneumonia and atrophic rhinitis, primarily associated with serogroups A, D, and F. Although serogroups A and D are prevalent in pigs and well\u2010established causes of these diseases, the pathogenicity and genomic characteristics of porcine serogroup F remain poorly characterized. Here, we isolated a virulent\n                    <jats:italic>P. multocida<\/jats:italic>\n                    strain\u2014AH01, from pigs with fatal acute respiratory disease in Anhui, China. It was characterized as a capsular Type F, lipopolysaccharide (LPS) antigen Type L3 isolate of sequence type (ST) 9. To evaluate the pathogenicity of this strain, pigs were challenged intratracheally with AH01 (6\u2009\u00d7\u200910\n                    <jats:sup>9<\/jats:sup>\n                    \u2009CFU), inducing acute pyrexia, dyspnea, anorexia, and rapid mortality (\u226412\u2009h postinfection, hpi). PacBio SMRT (Single\u2010Molecule Real Time) sequencing generated a complete 2.27\u2010Mbp chromosome (40.3% GC content; 2058 CDSs). Annotation identified 254 potential virulence\u2010associated genes, 47 different drug resistance phenotypes, and three genomic islands (GIs). Comparative genomics revealed a novel 16.7\u2010kb specific region insertion encoding zonula occludens toxin (Zot) and general secretion pathway protein D (GspD), potentially facilitating epithelial barrier disruption. Furthermore, polymorphisms in LPS outer core biosynthesis genes\n                    <jats:italic>natC<\/jats:italic>\n                    and\n                    <jats:italic>gatF<\/jats:italic>\n                    were characterized across strains avian Pm70, porcine AH01, and HN07. Strain AH01 harbors a single\u2010nucleotide deletion (\n                    <jats:italic>natC<\/jats:italic>\n                    position 760), causing a frameshift and premature stop. Both porcine strains AH01 and HN07 exhibited a 216\u2010bp N\u2010terminal extension in\n                    <jats:italic>gatF<\/jats:italic>\n                    compared to avian Pm70 strain, indicating host\u2010specific or strain\u2010dependent LPS biosynthetic divergence. Collectively, these findings provide critical insights into the pathogenicity and genomic basis of porcine\u2010derived\n                    <jats:italic>P. multocida<\/jats:italic>\n                    serogroup F.\n                  <\/jats:p>","DOI":"10.1155\/tbed\/9979547","type":"journal-article","created":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:43:52Z","timestamp":1762868632000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Pathogenicity and Genomic Characterization of\n                    <i>Pasteurella multocida<\/i>\n                    Serogroup F Isolate AH01 From Porcine Pneumonia in China"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5751-6468","authenticated-orcid":false,"given":"Yang-Yang","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai-Xia","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan-Hao","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Qiang","family":"Zhen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye-Qing","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shouyu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8688-095X","authenticated-orcid":false,"given":"Guang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-5638-9891","authenticated-orcid":false,"given":"Gairu","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,11]]},"reference":[{"key":"e_1_2_14_1_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00014-19"},{"key":"e_1_2_14_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2020.151417"},{"key":"e_1_2_14_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-019-1861-5"},{"key":"e_1_2_14_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02029-08"},{"key":"e_1_2_14_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.3.924-929.2001"},{"key":"e_1_2_14_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02824-14"},{"key":"e_1_2_14_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.25.4.615-618.1987"},{"key":"e_1_2_14_8_2","doi-asserted-by":"publisher","DOI":"10.2307\/1593009"},{"key":"e_1_2_14_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1743-1746.2003"},{"key":"e_1_2_14_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.152.1.7"},{"key":"e_1_2_14_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1480-1483.2005"},{"key":"e_1_2_14_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.2004.00807.x"},{"key":"e_1_2_14_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03654-23"},{"key":"e_1_2_14_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.05019-0"},{"key":"e_1_2_14_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2016.11.028"},{"key":"e_1_2_14_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2024.110046"},{"key":"e_1_2_14_17_2","first-page":"2254","article-title":"Epidemiological 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