{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T16:29:35Z","timestamp":1772987375313,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":350,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:italic>Babesia ovis<\/jats:italic>\n                    is a tick\u2010borne protozoan parasite that poses a significant threat to sheep production, particularly in endemic regions such as T\u00fcrkiye. It is known to be transmitted by ixodid ticks through both transstadial and transovarial routes. This study was aimed at investigating the natural transovarial transmission potential of\n                    <jats:italic>B. ovis<\/jats:italic>\n                    in\n                    <jats:italic>Rhipicephalus bursa<\/jats:italic>\n                    and\n                    <jats:italic>Rhipicephalus turanicus<\/jats:italic>\n                    ticks under field conditions. A total of 751 adult ticks were collected from 84 small ruminants (41 sheep and 43 goats) in Elaz\u0131\u011f Province, T\u00fcrkiye. Following species identification, 85 fully engorged female\n                    <jats:italic>R. bursa<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    = 45) and\n                    <jats:italic>R. turanicus<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    = 40) were incubated under controlled conditions to allow oviposition. DNA was extracted from the female carcasses and their corresponding larval pools and analyzed using nested PCR targeting the\n                    <jats:italic>18S rRNA<\/jats:italic>\n                    gene of\n                    <jats:italic>B. ovis<\/jats:italic>\n                    . The results revealed that\n                    <jats:italic>B. ovis<\/jats:italic>\n                    DNA was detected in 19.04% (8\/42) of\n                    <jats:italic>R. bursa<\/jats:italic>\n                    females and in 7.89% (3\/38) of\n                    <jats:italic>R. turanicus<\/jats:italic>\n                    females. Importantly, all larval pools derived from\n                    <jats:italic>B. ovis<\/jats:italic>\n                    \u2010positive\n                    <jats:italic>R. bursa<\/jats:italic>\n                    females also tested positive, indicating natural transovarial transmission. In contrast, none of the larval pools from\n                    <jats:italic>R. turanicus<\/jats:italic>\n                    tested positive, despite the presence of\n                    <jats:italic>B. ovis<\/jats:italic>\n                    in the female carcasses. These findings suggest that\n                    <jats:italic>R. bursa<\/jats:italic>\n                    may serve as a competent natural vector and reservoir for\n                    <jats:italic>B. ovis<\/jats:italic>\n                    , whereas\n                    <jats:italic>R. turanicus<\/jats:italic>\n                    seems to possess less ability for vertical transmission of the parasite.\n                  <\/jats:p>","DOI":"10.1155\/tswj\/2474599","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T12:28:46Z","timestamp":1765974526000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigation of Field\u2010Based Molecular Evidence of Natural Transovarial Transmission of\n                    <i>Babesia ovis<\/i>\n                    in\n                    <i>Rhipicephalus bursa<\/i>\n                    and\n                    <i>Rhipicephalus turanicus<\/i>\n                    Ticks"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4492-143X","authenticated-orcid":false,"given":"Mehmet Can","family":"Ulucesme","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3188-8757","authenticated-orcid":false,"given":"Munir","family":"Aktas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1086\/319347"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres\/2009020"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0065-308x(08)60285-7"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1079\/9780851996325.0029"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2013.06.009"},{"key":"e_1_2_10_6_2","first-page":"161","article-title":"General Review of Tick-Borne Diseases of Sheep and Goats World-Wide","volume":"39","author":"Uilenberg G.","year":"1997","journal-title":"Parassitologia"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2007.08.002"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2018.03.005"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani10112179"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10020231"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-022-07424-8"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2020.109034"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2012.07.025"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2025.1544775"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-011118-111932"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.32996\/bjbs.2024.4.2.3"},{"key":"e_1_2_10_17_2","first-page":"99","article-title":"Tick-Borne Diseases of Sheep and Goats Caused by Babesia, Theileria or Anaplasma spp.","volume":"39","author":"Friedhoff K. 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