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Sea cucumbers are recognized as rich sources of bioactive sulfated metabolites with potential antiparasitic properties; however, information on the chemistry and trypanocidal activity of Caribbean\n                    <jats:italic>Isostichopus<\/jats:italic>\n                    species is still scarce. In this study, the trypanocidal activity of solvent fractions from\n                    <jats:italic>Isostichopus<\/jats:italic>\n                    sp. aff.\n                    <jats:italic>badionotus<\/jats:italic>\n                    was evaluated, together with the chemical characterization and molecular docking analysis of their major metabolites. The methanolic extract, obtained by maceration of lyophilized tissue, was partitioned with\n                    <jats:italic>n<\/jats:italic>\n                    \u2010hexane, dichloromethane, and\n                    <jats:italic>n<\/jats:italic>\n                    \u2010butanol, and the resulting fractions were analyzed by UHPLC\u2013HRMS. The in vitro trypanocidal activity was assessed against\n                    <jats:italic>T. cruzi<\/jats:italic>\n                    epimastigotes, and molecular docking studies explored the interactions of five sulfated sterols (\n                    <jats:bold>1\u20135<\/jats:bold>\n                    ) with four key enzymes involved in the parasite\u2032s redox metabolism. Chemical profiling revealed that sulfated sterols, mainly cholestane\u2010 and stigmastane\u2010type derivatives, predominated in the dichloromethane fraction, which exhibited the strongest trypanocidal activity. Docking results indicated that stigmastane\u2010type sterols, particularly Compound\n                    <jats:bold>5<\/jats:bold>\n                    , showed the most favorable binding energies with all enzyme targets. This study provides the first evidence of trypanocidal activity in\n                    <jats:italic>Isostichopus<\/jats:italic>\n                    sp. aff.\n                    <jats:italic>badionotus<\/jats:italic>\n                    , highlighting sulfated sterols (\n                    <jats:bold>1\u20135<\/jats:bold>\n                    ) as plausible lead compounds for exploring agents against\n                    <jats:italic>T. cruzi<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1155\/tswj\/2849694","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T06:50:22Z","timestamp":1782802222000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring the Antiparasitic Activity of the Sea Cucumber\n                    <i>Isostichopus<\/i>\n                    sp. aff.\n                    <i>badionotus<\/i>\n                    From the Northern Coast of Colombia Against\n                    <i>Trypanosoma cruzi<\/i>"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9117-0405","authenticated-orcid":false,"given":"Manuel E.","family":"Taborda-Mart\u00ednez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2926-2813","authenticated-orcid":false,"given":"Ricardo","family":"Altamar-Ib\u00e1\u00f1ez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4935-876X","authenticated-orcid":false,"given":"Yeray A.","family":"Rodr\u00edguez-N\u00fa\u00f1ez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3553-9749","authenticated-orcid":false,"given":"Ericsson","family":"Coy-Barrera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1270-5326","authenticated-orcid":false,"given":"Adriana","family":"Rodr\u00edguez-Forero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2983-1727","authenticated-orcid":false,"given":"Jorge","family":"Soto-Delgado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9248-4596","authenticated-orcid":false,"given":"Lisandro","family":"Pacheco-Lugo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1754-6793","authenticated-orcid":false,"given":"Mitchell","family":"Bacho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9885-5071","authenticated-orcid":false,"given":"Fabi\u00e1n","family":"Espitia-Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,29]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.3389\/FPUBH.2019.00166"},{"key":"e_1_2_13_2_2","volume-title":"Chagas 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