{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T15:58:10Z","timestamp":1781798290831,"version":"3.54.5"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T00:00:00Z","timestamp":1781740800000},"content-version":"vor","delay-in-days":168,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Cayratia trifolia<\/jats:italic>\n                    is traditionally used for treating pain, inflammation, and oxidative disorders, yet comprehensive experimental and computational validation of its pharmacological potential remains limited. The aim of this study is to investigate the phytochemical profile and evaluate antioxidant, anti\u2010inflammatory, and analgesic activities of methanolic and n\u2010hexane fractions of\n                    <jats:italic>C. trifolia<\/jats:italic>\n                    leaves using integrated in vitro, in vivo, and in silico approaches. Leaves were extracted with methanol and fractionated via a modified Kupchan method. Phytochemical screening, DPPH radical scavenging, HRBC membrane stabilization, carrageenan\u2010induced paw edema, acetic acid\u2010induced writhing, and tail immersion assays were performed. In silico analysis included PASS prediction, ADME\/T profiling, and molecular docking of 40 previously documented GC\u2013MS compounds against COX\u20102 (PDB: 6COX, 1CX2, 5IKR, and 1CQE) and CYP2C9 (PDB: 1OG5 and 4L7B) protein targets. Methanolic extract demonstrated potent antioxidant activity (IC\n                    <jats:sub>50<\/jats:sub>\n                    = 2.69\n                    <jats:italic>\u2009\u03bc<\/jats:italic>\n                    g\/mL) and 87.46% HRBC membrane stabilization. Significant anti\u2010inflammatory effects were observed in vivo through reduced paw edema. The n\u2010hexane fraction produced notable analgesic responses, inhibiting writhing by 21.84% and increasing reaction time in the tail immersion test by 59.44% (\u2009\n                    <jats:sup>\u2217\u2217\u2217\u2217<\/jats:sup>\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.0001). In silico analyses identified multiple bioactive compounds\u2014especially 2\u2010cyclohexylethyl isobutyl ester and lup\u201020(29)\u2010en\u20103\u2010yl acetate\u2014with binding affinities surpassing diclofenac, aspirin, and ascorbic acid, along with favourable ADME\/T profiles. The combined experimental and computational findings indicate that\n                    <jats:italic>C. trifolia<\/jats:italic>\n                    contains phytoconstituents with strong therapeutic potential. Its methanolic fraction, supported by in silico validation, represents a promising source for developing natural antioxidant, anti\u2010inflammatory, and analgesic lead molecules.\n                  <\/jats:p>","DOI":"10.1155\/tswj\/8390762","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T15:37:21Z","timestamp":1781797041000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Phytochemical, Pharmacological, and In Silico Evaluation of the Methanolic Extract of\n                    <i>Cayratia trifolia<\/i>\n                    Leaves: Antioxidant, Anti\u2010Inflammatory, and Analgesic Activities"],"prefix":"10.1155","volume":"2026","author":[{"given":"Nasrin","family":"Jubayda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. 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