{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T08:36:24Z","timestamp":1789202184288,"version":"build-2803163510"},"reference-count":67,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"vor","delay-in-days":13,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Aleuritopteris bicolor<\/jats:italic>\n                    (Family: Pteridaceae; Nepalese name: Raani Sinka) is an edible fern native to Nepal, India, China, Bangladesh, Sri Lanka, and Pakistan. Ethnomedicinal practitioners from various ethnic tribes in Nepal have traditionally prescribed it to heal wounds, diarrhea, dysentery, and gastritis. However, scientific evidence supporting these efficacies remains limited until January 2025. In the present study, we aimed to validate these traditional uses through chemical, in vitro, and in silico analyses of the plant\u2032s hydroalcoholic extract. The 70% methanolic extract of the plant exhibited potent DPPH free radical scavenging activity with an IC\n                    <jats:sub>50<\/jats:sub>\n                    of 20.54 \u00b1 4.4\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g\/mL. The extract also demonstrated potent and dose\u2010dependent antibacterial activity against\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (zone of inhibition: 8\u201312\u2009mm and minimum inhibitory concentration: 3125\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g\/mL), assessed using well diffusion and broth microdilution techniques. However, the plant extract was found to be a weak inhibitor of the enzyme alpha\u2010amylase. Phytochemical analysis using LC\u2010MS revealed the presence of chlorogenic acid, kumatakenin, quercetin 3\u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010glucoside, and rhamnocitrin 3\u2010\n                    <jats:italic>O<\/jats:italic>\n                    \u2010glucoside; which, when docked against penicillin\u2010binding protein\u2032s catalytic residues (SER 403, LYS 406, SER 462, ASN 464, and THR 600), showed binding energies ranging from \u22126.3 to \u22127.1\u2009kcal\/mol, suggesting prominent molecular interactions compared with meropenem antibiotics. Furthermore, kumatakenin satisfied Lipinski\u2032s parameters for drug\u2010likeness, indicating its great potential as a drug candidate. The current study provides scientific evidence for the potent phytoconstituents with antioxidant and antibacterial potential of\n                    <jats:italic>A. bicolor<\/jats:italic>\n                    for the first time, highlighting its potential for topical application in treating bacterial infectious wounds.\n                  <\/jats:p>","DOI":"10.1155\/tswj\/8851368","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T08:12:22Z","timestamp":1768378342000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Antioxidant, Antibacterial, and Antidiabetic Activities of\n                    <i>Aleuritopteris bicolor<\/i>\n                    From Nepal: A LC\u2010MS, In Vitro, and In Silico Investigations to Establish Its Potential as a Therapeutic Candidate"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9481-1087","authenticated-orcid":false,"given":"Rekha","family":"Bhandari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9972-7419","authenticated-orcid":false,"given":"Shailendra Kumar","family":"Sharma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0807-2710","authenticated-orcid":false,"given":"Peru Kumari","family":"Bishwakarma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3913-6044","authenticated-orcid":false,"given":"Sadikshya","family":"Sapkota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8722-7294","authenticated-orcid":false,"given":"Ram Kishor","family":"Yadav","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harish Babu","family":"P. C.","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7424-222X","authenticated-orcid":false,"given":"Sandesh","family":"Poudel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sajan L.","family":"Shyaula","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5155-6098","authenticated-orcid":false,"given":"Khem Raj","family":"Joshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,14]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1177\/1934578X251340467"},{"key":"e_1_2_11_2_2","first-page":"3","volume-title":"New Look to Phytomedicine Chattopadhyay","author":"Khan A.","year":"2019"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.111859"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2017.50.3.221"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0319338"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2022.103797"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.59049\/2790-0231.10.2.2311"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.4672"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sciaf.2021.e01091"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/8178294"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-021-03702-w"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13080746"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/foods13050660"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1177\/1934578X251318518"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.35516\/jjps.v18i2.2691"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.32859\/era.22.16.1-16"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/medicines6020069"},{"key":"e_1_2_11_18_2","first-page":"39","article-title":"Phytochemical Screening , Free Radical Scavenging and In-Vitro Anti-Bacterial Activity of Ethanolic Extracts of Selected Medicinal Plants of Nepal and Effort Towards Formulation of Antibacterial Cream From the Extracts","volume":"9","author":"Tiwari R.","year":"2021","journal-title":"International Journal of Herbal Medicine"},{"key":"e_1_2_11_19_2","doi-asserted-by":"publisher","DOI":"10.1155\/2024\/5623028"},{"key":"e_1_2_11_20_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27092688"},{"key":"e_1_2_11_21_2","doi-asserted-by":"publisher","DOI":"10.35516\/jjps.v17i4.2532"},{"key":"e_1_2_11_22_2","doi-asserted-by":"publisher","DOI":"10.1155\/2024\/1119165"},{"key":"e_1_2_11_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2020.08.015"},{"key":"e_1_2_11_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm050362n"},{"key":"e_1_2_11_25_2","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2014.915829"},{"key":"e_1_2_11_26_2","doi-asserted-by":"publisher","DOI":"10.1248\/cpb.c13-00748"},{"key":"e_1_2_11_27_2","doi-asserted-by":"publisher","DOI":"10.1177\/1934578X1300800508"},{"key":"e_1_2_11_28_2","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2020.507887"},{"key":"e_1_2_11_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.inoche.2020.108140"},{"key":"e_1_2_11_30_2","doi-asserted-by":"publisher","DOI":"10.53941\/jmnp.2025.100004"},{"key":"e_1_2_11_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0326808"},{"key":"e_1_2_11_32_2","first-page":"1","article-title":"Journal of Nepal Pharmaceutical Association","volume":"28","author":"Devkota H. P.","year":"2017","journal-title":"Journal of Nepal Pharmaceutical Association"},{"key":"e_1_2_11_33_2","first-page":"19","article-title":"Principles and Applications of Liquid Chromatography-Mass Spectrometry in Clinical Biochemistry","volume":"30","author":"Pitt J. J.","year":"2009","journal-title":"Clinical Biochemist Reviews"},{"key":"e_1_2_11_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-017-1626-y"},{"key":"e_1_2_11_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(00)00164-1"},{"key":"e_1_2_11_36_2","doi-asserted-by":"publisher","DOI":"10.1080\/10826076.2015.1091011"},{"key":"e_1_2_11_37_2","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/968314"},{"key":"e_1_2_11_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2006.06.006"},{"key":"e_1_2_11_39_2","unstructured":"VermaD. D. Antioxidative Properties of Flavonoids from Cheilanthes Anceps Swartz Journal of American Science 2010 6 no. 5."},{"key":"e_1_2_11_40_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules23040803"},{"key":"e_1_2_11_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(01)00226-4"},{"key":"e_1_2_11_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2021.103345"},{"key":"e_1_2_11_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2022.104310"},{"key":"e_1_2_11_44_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-015-0580-0"},{"key":"e_1_2_11_45_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.5b00471"},{"key":"e_1_2_11_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.2010.02267.x"},{"key":"e_1_2_11_47_2","doi-asserted-by":"publisher","DOI":"10.3390\/ph15030385"},{"key":"e_1_2_11_48_2","doi-asserted-by":"publisher","DOI":"10.4081\/mr.2017.6877"},{"key":"e_1_2_11_49_2","doi-asserted-by":"publisher","DOI":"10.1002\/fsn3.3615"},{"key":"e_1_2_11_50_2","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/814590"},{"key":"e_1_2_11_51_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12111645"},{"key":"e_1_2_11_52_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph15102321"},{"key":"e_1_2_11_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2017.02.004"},{"key":"e_1_2_11_54_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27082494"},{"key":"e_1_2_11_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/spectrum.03934-23"},{"key":"e_1_2_11_56_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/746165"},{"key":"e_1_2_11_57_2","doi-asserted-by":"publisher","DOI":"10.3390\/plants9070852"},{"key":"e_1_2_11_58_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.1865"},{"key":"e_1_2_11_59_2","doi-asserted-by":"publisher","DOI":"10.1515\/jbcpp-2013-0141"},{"key":"e_1_2_11_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2024.06.007"},{"key":"e_1_2_11_61_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules28207008"},{"key":"e_1_2_11_62_2","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics14091818"},{"key":"e_1_2_11_63_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0309797"},{"key":"e_1_2_11_64_2","doi-asserted-by":"publisher","DOI":"10.1080\/2314808X.2022.2050115"},{"key":"e_1_2_11_65_2","doi-asserted-by":"publisher","DOI":"10.3390\/ph17060757"},{"key":"e_1_2_11_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rechem.2023.101278"},{"key":"e_1_2_11_67_2","doi-asserted-by":"publisher","DOI":"10.3390\/pr8070858"}],"container-title":["The Scientific World Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tswj\/8851368","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/tswj\/8851368","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/tswj\/8851368","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T08:12:25Z","timestamp":1768378345000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/tswj\/8851368"}},"subtitle":[],"editor":[{"given":"Basavarajaiah","family":"S. M.","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":67,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/tswj\/8851368"],"URL":"https:\/\/doi.org\/10.1155\/tswj\/8851368","archive":["Portico"],"relation":{},"ISSN":["2356-6140","1537-744X"],"issn-type":[{"value":"2356-6140","type":"print"},{"value":"1537-744X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-01-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"8851368"}}