{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T14:50:19Z","timestamp":1772981419962,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"vor","delay-in-days":187,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100017170","name":"Thailand Science Research and Innovation","doi-asserted-by":"publisher","award":["201067"],"award-info":[{"award-number":["201067"]}],"id":[{"id":"10.13039\/501100017170","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine International"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Wild felids have been in decline, which has been linked to health issues. Parasitic infections, including zoonotic ones, can contribute to the challenges faced regarding wildlife conservation efforts and may pose a risk to human health, particularly for people working at or visiting zoological parks. This study investigated the occurrence of parasitic infections in the Felidae within Thai zoo parks.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    The fecal samples were collected from 93 Felidae individuals residing in four zoo parks. The parasitological technique involved sugar floatation, and a formalin\u2010ethyl acetate concentration technique (FECT) was also employed. Additionally, a molecular technique was used to detect\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    DNA in feces. For blood samples, 22 were tested for\n                    <jats:italic>T. gondii<\/jats:italic>\n                    DNA using the PCR technique. To detect\n                    <jats:italic>T. gondii<\/jats:italic>\n                    antibodies in serum, an indirect fluorescent antibody test (IFAT) was performed, and the result was confirmed using an enzyme\u2010linked immunosorbent assay (ELISA).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    The prevalence of intestinal parasites in captive felids was 23.7%, with the highest rates observed for hookworms (8.6%),\n                    <jats:italic>Toxascaris leonina<\/jats:italic>\n                    (7.5%), coccidia (4.3%)\n                    <jats:italic>, Strongyloides<\/jats:italic>\n                    spp. (2.1%), and\n                    <jats:italic>Opisthorchis<\/jats:italic>\n                    \u2010like egg (1.1%). Among the Felidae, lions and white lions (\n                    <jats:italic>Panthera leo<\/jats:italic>\n                    ) exhibited the highest prevalence of parasitic infection, particularly\n                    <jats:italic>T. leonina<\/jats:italic>\n                    . Furthermore, a substantial percentage (63.6%) of the animals tested positive for\n                    <jats:italic>T. gondii<\/jats:italic>\n                    antibodies using both IFAT and ELISA.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    These findings highlight the importance of addressing the circulation of parasites, such as\n                    <jats:italic>T<\/jats:italic>\n                    .\n                    <jats:italic>gondii<\/jats:italic>\n                    , hookworm,\n                    <jats:italic>T. leonina<\/jats:italic>\n                    , and coccidia in environments where animals and humans closely interact, such as wildlife zoos. Implementing preventative measures and adopting a one\u2010health approach are strongly encouraged to control parasites and reduce the risk of infection for animals and humans.\n                  <\/jats:p>","DOI":"10.1155\/vmi\/1596677","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T08:04:00Z","timestamp":1751875440000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Epidemiological Surveillance of Intestinal Parasites and Serological Analysis of\n                    <i>Toxoplasma gondii<\/i>\n                    in Captive Felids From Thailand Zoos"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8058-9406","authenticated-orcid":false,"given":"Nuttanan","family":"Hongsrichan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4289-2762","authenticated-orcid":false,"given":"Peerawich","family":"Donthaisong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4483-571X","authenticated-orcid":false,"given":"Chavin","family":"Chaisongkram","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6193-9842","authenticated-orcid":false,"given":"Chatanun","family":"Eamudomkarn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1401-3385","authenticated-orcid":false,"given":"Opal","family":"Pitaksakulrat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9989-5848","authenticated-orcid":false,"given":"Kanda","family":"Ponsrila","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4215-3702","authenticated-orcid":false,"given":"Bandid","family":"Mangkit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5056-0493","authenticated-orcid":false,"given":"Piangjai","family":"Chalermwong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5052-2358","authenticated-orcid":false,"given":"Thitichai","family":"Jarudecha","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-7746-5124","authenticated-orcid":false,"given":"Achirawit","family":"Surapinit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9763-5720","authenticated-orcid":false,"given":"Thidarut","family":"Boonmars","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0833-3729","authenticated-orcid":false,"given":"Rucksak","family":"Rucksaken","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,7]]},"reference":[{"key":"e_1_2_14_1_2","first-page":"467","volume-title":"Fowler\u2019s Zoo and Wild Animal Medicine","author":"Lamberski N.","year":"2015"},{"key":"e_1_2_14_2_2","doi-asserted-by":"publisher","DOI":"10.1645\/GE-2502.1"},{"key":"e_1_2_14_3_2","doi-asserted-by":"publisher","DOI":"10.1645\/GE-1453.1"},{"key":"e_1_2_14_4_2","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2018.2385"},{"key":"e_1_2_14_5_2","doi-asserted-by":"publisher","DOI":"10.1645\/GE-3519RN.1"},{"key":"e_1_2_14_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10344-007-0116-0"},{"key":"e_1_2_14_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-022-03425-y"},{"key":"e_1_2_14_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/1749-4877.12342"},{"key":"e_1_2_14_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.287.5452.443"},{"key":"e_1_2_14_10_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1401.071113"},{"key":"e_1_2_14_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijppaw.2020.03.006"},{"key":"e_1_2_14_12_2","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-30.3.472"},{"key":"e_1_2_14_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-165x.2010.00216.x"},{"key":"e_1_2_14_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2015.10.004"},{"key":"e_1_2_14_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2005.12.009"},{"key":"e_1_2_14_16_2","first-page":"15","article-title":"Comparison of Common Fecal Flotation Techniques for the Recovery of Parasite Eggs and Oocysts","volume":"6","author":"Dryden M. 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