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Thus, this study aims to determine EHV\u20101\/\u20104 prevalence in Colombian creole horses from Ibagu\u00e9, Tolima, using molecular techniques, as well as to determine possible risk factors associated with viral infection. A cross\u2010sectional study was carried out in 110 equines blood, serum, and semen samples from Ibagu\u00e9, Tolima. Antibodies against EHV\u20101\/\u20104 were determined through indirect ELISA. EHV\u20101 was detected by amplifying the glycoprotein H gene through heminested PCR, and risk factors were calculated with chi\u2010square, odds ratio, and fisher test. EHV\u20101\/\u20104 antibodies were found in 11.8% of the horses. Moreover, the presence of EHV\u20101 and EHV\u20104 was determined in blood, serum, and semen of healthy clinical equines, with a prevalence infection in horses of 43.36% for EHV\u20101 and 2.7% for EHV\u20104. Sex, age, births number, abortions, natural breeding, and artificial insemination did not show significance as risk factors. This study determined a high prevalence of EHV\u20101 and low prevalence of EHV\u20104 in horses from Ibagu\u00e9, Tolima. Furthermore, the possibility of finding the infectious agent in blood, serum, and semen was demonstrated. We recommend the use of blood and serum samples for virus detection and the development of new studies to assess EHV\u20101 infection feasibility in venereal transmissions.<\/jats:p>","DOI":"10.1155\/vmi\/1661949","type":"journal-article","created":{"date-parts":[[2025,1,29]],"date-time":"2025-01-29T10:52:37Z","timestamp":1738147957000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Molecular and Serological Investigation of Equine Herpesvirus Type 1 (EHV\u20101) and Type 4 (EHV\u20104) in Horses In Ibagu\u00e9, Tolima"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7473-3472","authenticated-orcid":false,"given":"Julieth 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