{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T05:20:50Z","timestamp":1787548850786,"version":"build-2736575974"},"reference-count":67,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T00:00:00Z","timestamp":1787443200000},"content-version":"vor","delay-in-days":234,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005362","name":"Universita degli Studi di Bari Aldo Moro","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005362","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Avian polyomavirus (APyV) is responsible for diseases in birds belonging to several avian orders, including Psittaciformes, Anseriformes, and other susceptible taxa. APyVs appear to be able to replicate in various organs of susceptible bird species. This study aimed to investigate (i) the distribution of APyV in the organs of two parrot chicks belonging to different species, Moluccan eclectus (\n                    <jats:italic>Eclectus roratus<\/jats:italic>\n                    ) and blue\u2010winged macaw (\n                    <jats:italic>Primolius maracana<\/jats:italic>\n                    ), which died due to APyV infection and (ii) the lesions associated with the infection. By negative\u2010staining electron microscopy, well\u2010defined viral particles morphologically compatible with polyomavirus and polyomavirus\u2010like particles were identified in organs. Detection of APyV was confirmed by real\u2010time PCR, and BLAST analysis of the complete genome revealed 99.96% identity with a budgerigar fledging disease polyomavirus strain. The amount of DNA copy number\/ng of DNA ranged from 2.47\u2009\u00d7\u200910\n                    <jats:sup>3<\/jats:sup>\n                    found in the myocardium to 3.90\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    found in the feather quills of the eclectus and from 1.07\u2009\u00d7\u200910\n                    <jats:sup>1<\/jats:sup>\n                    to 1.82\u2009\u00d7\u200910\n                    <jats:sup>7<\/jats:sup>\n                    detected in the brain and intestine of the blue\u2010winged macaw, respectively. According to the findings of inclusion bodies, viral particles, and DNA, the lung appeared as one of the most severely affected tissues. Even if inclusion bodies were not detected, severe lesions were found in the myocardium, bursa of Fabricius, and spleen. The results of this study highlight the ability of APyV to spread widely, leading to severe lesions in the organs of infected birds. The most significant amount of viral DNA detected in the gut and feathers highlights the relevance of feces and feathers as useful samples for the in vivo diagnosis.\n                  <\/jats:p>","DOI":"10.1155\/vmi\/2135685","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T05:01:45Z","timestamp":1787547705000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Polyomavirus Infection in Two Young Parrots: Assessment of Viral Distribution in Organs and Affected 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