{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T07:00:38Z","timestamp":1780988438284,"version":"3.54.1"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,6,23]],"date-time":"2025-06-23T00:00:00Z","timestamp":1750636800000},"content-version":"vor","delay-in-days":173,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005621","name":"Kasetsart University Research and Development Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005621","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004539","name":"Kasetsart University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004539","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine International"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Zoonotic malaria, caused by simian\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    spp., poses a major public health challenge in Southeast Asia, including Thailand, where long\u2010tailed macaques (\n                    <jats:italic>Macaca fascicularis<\/jats:italic>\n                    ) serve as natural reservoirs. This study investigated the molecular prevalence and species identification of zoonotic simian\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    spp. in macaques from four provinces in Southern Thailand: Phetchaburi, Satun, Phang Nga, and Surat Thani. A total of 310 blood samples were collected between May 2023 and June 2024 and analyzed using nested and seminested polymerase chain reaction (PCR) techniques targeting the 18S rRNA gene. Sequencing analyses confirmed the presence of zoonotic\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    species. Overall, 11.3% (35\/310; 95% CI: 7.9\u201315.3) of the macaques tested positive, with\n                    <jats:italic>Plasmodium inui<\/jats:italic>\n                    being the most prevalent species at 9.4% (29\/310), followed by\n                    <jats:italic>Plasmodium knowlesi<\/jats:italic>\n                    and\n                    <jats:italic>Plasmodium cynomolgi<\/jats:italic>\n                    , each at 0.9% (3\/310). The highest prevalence was observed in Surat Thani at 18% (18\/100). These findings underscore the zoonotic potential of simian malaria and its geographic distribution in Southern Thailand, which may be associated with the significant increase in macaque populations and their expanding habitat overlap with human communities. In conclusion, this study highlights the major role of long\u2010tailed macaques as reservoirs for zoonotic\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    spp. Enhanced surveillance and community awareness are crucial for mitigating cross\u2010species transmission and improving malaria control.\n                  <\/jats:p>","DOI":"10.1155\/vmi\/3024193","type":"journal-article","created":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T00:19:59Z","timestamp":1750724399000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular Prevalence and Identification of Zoonotic\n                    <i>Plasmodium<\/i>\n                    spp., Including\n                    <i>Plasmodium knowlesi<\/i>\n                    ,\n                    <i>Plasmodium cynomolgi<\/i>\n                    , and\n                    <i>Plasmodium inui<\/i>\n                    , in Long\u2010Tailed Macaques (\n                    <i>Macaca fascicularis<\/i>\n                    ) of Southern Thailand"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1566-4159","authenticated-orcid":false,"given":"Thanawat","family":"Hmaidee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0833-3729","authenticated-orcid":false,"given":"Rucksak","family":"Rucksaken","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8191-4415","authenticated-orcid":false,"given":"Supakarn","family":"Kaewchot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1018-0529","authenticated-orcid":false,"given":"Piya","family":"Sereerak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6748-0792","authenticated-orcid":false,"given":"Salintorn","family":"Thongsahuan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5052-2358","authenticated-orcid":false,"given":"Thitichai","family":"Jarudecha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3312-7246","authenticated-orcid":false,"given":"Sakulchit","family":"Wichainchot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1084-0298","authenticated-orcid":false,"given":"Phakorn","family":"Wilaisri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9407-221X","authenticated-orcid":false,"given":"Chanapath","family":"Thabthimsri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5869-5714","authenticated-orcid":false,"given":"Perm","family":"Premphoolsawat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9552-2335","authenticated-orcid":false,"given":"Wanat","family":"Sricharern","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,23]]},"reference":[{"key":"e_1_2_10_1_2","unstructured":"WHO Malaria 2023 https:\/\/www.who.int\/health-topics\/malaria#tab=tab_1."},{"key":"e_1_2_10_2_2","volume-title":"Malaria Control During Mass Population Movements and Natural Disasters","author":"Bloland P. B.","year":"2002"},{"key":"e_1_2_10_3_2","volume-title":"Malaria","author":"World Health Organization","year":"2024"},{"key":"e_1_2_10_4_2","first-page":"1","article-title":"Vector-Borne Disease Problems in Rapid Urbanization: New Approaches to Vector Control","volume":"70","author":"Knudsen A. B.","year":"1992","journal-title":"Bulletin of the World Health Organization"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.11.177"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40101-020-00251-9"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/ecog.01158"},{"key":"e_1_2_10_8_2","volume-title":"Avian Malaria Parasites and Other Haemosporina","author":"Gediminas V.","year":"2005"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1017\/s0031182017000737"},{"key":"e_1_2_10_10_2","first-page":"218","article-title":"Malaria and Hemogregarines From Lizards of the Western Caribbean Islands of San Andr\u00e9s and Providencia","volume":"17","author":"Ayala S. C.","year":"1975","journal-title":"Revista do Instituto de Medicina Tropical de Sao Paulo"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.2307\/3279261"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01099"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-13-68"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-020-03424-0"},{"key":"e_1_2_10_15_2","first-page":"2","article-title":"An Updated Taxonomy and Conservation Status Review of Asian Primates","volume":"4","author":"Roos C.","year":"2014","journal-title":"Asian Primates Journal"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.16966\/2473-1846.127"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1080\/23144599.2022.2040176"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10050629"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2016.02.004"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.14202\/vetworld.2021.884-888"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-024-06500-5"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2702.191660"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2020.05.022"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1710.110349"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-11-36"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2023.107030"},{"key":"e_1_2_10_27_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.60.687"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiy519"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2020.105596"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-019-2780-4"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2024.107187"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-016-1494-0"},{"key":"e_1_2_10_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-015-0856-3"},{"key":"e_1_2_10_34_2","first-page":"621","article-title":"Use and Limitations of Light Microscopy for Diagnosing Malaria at the Primary Health Care Level","volume":"66","author":"Payne D.","year":"1988","journal-title":"Bulletin of the World Health Organization"},{"key":"e_1_2_10_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2006.01779.x"},{"key":"e_1_2_10_36_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-5-121"},{"key":"e_1_2_10_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002015"},{"key":"e_1_2_10_38_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciab1060"},{"key":"e_1_2_10_39_2","unstructured":"BangprapaM. P. knowlesiMalaria \u2018On the Rise\u2019: Bangkok Post PCL 2022 https:\/\/www.bangkokpost.com\/thailand\/general\/2306078\/p-knowlesi-malaria-on-the-rise."},{"key":"e_1_2_10_40_2","unstructured":"PetpailinP. 11 Malaria Patients Found in Thailand\u2019s East Blood From Monkeys on Koh Chang Tested 2022 https:\/\/thethaiger.com\/news\/national\/11-malaria-patients-found-in-thailands-east-blood-from-monkeys-on-koh-chang-tested."},{"key":"e_1_2_10_41_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2708.204502"},{"key":"e_1_2_10_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msy158"},{"key":"e_1_2_10_43_2","doi-asserted-by":"publisher","DOI":"10.1080\/20477724.2020.1722434"},{"key":"e_1_2_10_44_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2912.230637"},{"key":"e_1_2_10_45_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.23-0349"},{"key":"e_1_2_10_46_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-6-26"}],"container-title":["Veterinary Medicine International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/vmi\/3024193","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/vmi\/3024193","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/vmi\/3024193","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T08:15:38Z","timestamp":1772957738000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/vmi\/3024193"}},"subtitle":[],"editor":[{"given":"Mahmoud","family":"Elhaig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1155\/vmi\/3024193"],"URL":"https:\/\/doi.org\/10.1155\/vmi\/3024193","archive":["Portico"],"relation":{},"ISSN":["2090-8113","2042-0048"],"issn-type":[{"value":"2090-8113","type":"print"},{"value":"2042-0048","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2025-01-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-06-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"3024193"}}