{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T23:48:12Z","timestamp":1787615292134,"version":"build-2736575974"},"reference-count":93,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T00:00:00Z","timestamp":1764979200000},"content-version":"vor","delay-in-days":339,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009775","name":"Red Meat Research and Development South Africa","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009775","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine International"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    This study determined the prevalence, characteristics and factors associated with isolating\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    and other\n                    <jats:italic>Listeria<\/jats:italic>\n                    species (\n                    <jats:italic>Listeria<\/jats:italic>\n                    spp.) from samples collected from cattle farms in Mpumalanga province, South Africa. A total of 475 samples comprising fresh faeces, pooled environmental faeces, silage, feeds and water were collected from 25 farms (feedlot, cow\u2013calf operations and communal) in three districts (Bronkhorstspruit, Emalahleni and Middleburg). Standard bacteriological and molecular assays were used to isolate, identify and characterize\n                    <jats:italic>Listeria<\/jats:italic>\n                    isolates. The prevalence of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    and other\n                    <jats:italic>Listeria<\/jats:italic>\n                    spp. in farm samples was 2.5% (12\/475) and 9.2% (44\/475) (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05), respectively. The highest prevalence of isolation of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    and\n                    <jats:italic>Listeria<\/jats:italic>\n                    spp. was 5.9% (5\/85) and 16.7% (5\/30) in faeces and silage, respectively. Farm size was the only factor significantly (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05) associated with detecting\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    ; the only serotype detected was 1\/2a, and all the isolates were positive for virulence genes\n                    <jats:italic>hlyA<\/jats:italic>\n                    and\n                    <jats:italic>inlJ.<\/jats:italic>\n                    The overall prevalence of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    in samples collected from cattle farms (2.5%) across the province, the detection of serotype 1\/2a associated with human listeriosis and the positivity of all strains for one or more virulent genes all pose significant public health and food safety risks from the sources assessed. There is a need to implement measures to reduce or eliminate carriage or contamination by\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    on cattle farms to avoid the entry of the pathogen into the human food chain in South Africa.\n                  <\/jats:p>","DOI":"10.1155\/vmi\/4708466","type":"journal-article","created":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T10:03:46Z","timestamp":1765015426000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Detection and Molecular Characterization of\n                    <i>Listeria monocytogenes<\/i>\n                    and\n                    <i>Listeria<\/i>\n                    spp. Isolates Recovered From Cattle Farms in Mpumalanga Province, South Africa"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7470-4268","authenticated-orcid":false,"given":"Khomotso Confidence","family":"Moabelo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8521-235X","authenticated-orcid":false,"given":"Nomakorinte","family":"Gcebe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9415-5297","authenticated-orcid":false,"given":"James","family":"Gana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7559-6617","authenticated-orcid":false,"given":"Yusuf Bitrus","family":"Ngoshe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebone","family":"Moerane","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9470-9421","authenticated-orcid":false,"given":"Abiodun Adewale","family":"Adesiyun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,6]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2019.103280"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jafr.2020.100082"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2016.12.016"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2021.01.068"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.00949-17.2017"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1088\/1755-1315\/85\/1\/012069"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2010.04.003"},{"key":"e_1_2_12_8_2","first-page":"2016","article-title":"Immunohistopathological and Immunological Evaluation of Listeria Nanoparticle Vaccine During the First and Third Semester in the Pregnancy Rat Model","volume":"20","author":"Al-Bayati H. 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