{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T11:56:12Z","timestamp":1791374172966,"version":"4.3.1"},"reference-count":63,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:00:00Z","timestamp":1791331200000},"content-version":"vor","delay-in-days":279,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Edwardsiella<\/jats:italic>\n                    species are reassigned under the family Hafniaceae following phylogenomic revisions of the order\n                    <jats:italic>Enterobacterales<\/jats:italic>\n                    , a primary pathogenic strain of fish, while\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    are members of the\n                    <jats:italic>Enterobacteriaceae<\/jats:italic>\n                    family that are frequently reported as contaminants with rare causes of illness in fish but severely affect other mammals. A cross\u2010sectional study was conducted from April to November 2022 with the objectives of estimating the prevalence of\n                    <jats:italic>Edwardsiella tarda<\/jats:italic>\n                    ,\n                    <jats:italic>Edwardsiella ictaluri<\/jats:italic>\n                    , and\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157:H7 in fish and determining the antimicrobial susceptibility pattern of the isolates. A total of 768 tissue samples comprising intestine, liver, and kidney were collected from 256 apparently healthy fish. Bacterial isolation was performed using selective and differential culture media, including MacConkey agar, xylose lysine deoxycholate (XLD) agar,\n                    <jats:italic>Edwardsiella<\/jats:italic>\n                    isolation medium, eosin methylene blue (EMB) agar, and sorbitol MacConkey agar. Further identification was performed using biochemical tests and latex agglutination assays, particularly for the targeted strain of\n                    <jats:italic>E. coli<\/jats:italic>\n                    . The disk diffusion test was employed to determine the susceptibility pattern of isolates against seven standard antimicrobial disks. The findings were presented using descriptive statistics, while the association of categorical variables with the occurrence of bacterial species was computed using chi\u2010square and logistic regression analysis. The overall prevalence of\n                    <jats:italic>E. tarda, E. ictaluri,<\/jats:italic>\n                    and\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157:H7 among fish was 8.6%, 4.7%, and 14.1%, respectively, and among organs (intestine, liver, and kidney) was 4.01%, 1.95%, and 5.34%, respectively. The maturity of fish and lake showed a significant association (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05) with the occurrence of\n                    <jats:italic>E. tarda<\/jats:italic>\n                    . Resistance to tetracycline was noted in all of the isolates. The results of this study concluded that the intestines of fish harbor\n                    <jats:italic>E. tarda, E. ictaluri,<\/jats:italic>\n                    as primary inhabitant pathogens, and\n                    <jats:italic>E. coli<\/jats:italic>\n                    O157:H7, which may be a transient microorganism mostly associated with fault handling practice.\n                  <\/jats:p>","DOI":"10.1155\/vmi\/4857001","type":"journal-article","created":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T11:34:42Z","timestamp":1791372882000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation an Antimicrobial Susceptibility Profile of\n                    <i>Edwardsiella<\/i>\n                    Species and\n                    <i>Escherichia coli<\/i>\n                    O157:H7 From Fish and Their Handling Environment in Lake Abaya and Gilgel Gibe\u20103 Dam, Southern Ethiopia"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-2196-6866","authenticated-orcid":false,"given":"Belay Walelign","family":"Moges","sequence":"first","affiliation":[{"name":"Department of Veterinary Science College of Veterinary Medicine and Animal Science Jinka University  Jinka Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1495-2972","authenticated-orcid":false,"given":"Jelalu Kemal","family":"Birmeka","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Haramaya University  Haramaya Ethiopia  haramaya.edu.et"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6059-6457","authenticated-orcid":false,"given":"Bruk Abraha","family":"Fitwi","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Haramaya University  Haramaya Ethiopia  haramaya.edu.et"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0888-8221","authenticated-orcid":false,"given":"Bulako Chebo","family":"Boke","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Wolaita Sodo University  Wolaita Sodo Ethiopia  wsu.edu.et"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6981-6046","authenticated-orcid":false,"given":"Tesfahun Demeke","family":"Dana","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Wolaita Sodo University  Wolaita Sodo Ethiopia  wsu.edu.et"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6277-9038","authenticated-orcid":false,"given":"Tesfaye Bekele","family":"Kassa","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine Wolaita Sodo University  Wolaita Sodo Ethiopia  wsu.edu.et"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,7]]},"reference":[{"key":"e_1_2_12_1_2","article-title":"Status of Lake Tana Commercial Fishery Ethiopia","volume":"1","author":"Dereje T.","year":"2013","journal-title":"Amhara Regional Agricultural Research Institute (ARARI)"},{"key":"e_1_2_12_2_2","article-title":"Fish Production, Consumption and Management in Ethiopia","volume":"3","author":"Janko A. M.","year":"2014","journal-title":"International Journal of Economics and Management"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0905235106"},{"key":"e_1_2_12_4_2","first-page":"75","article-title":"Assessment of Post-harvest Loss of Wet Fish: a Novel Approach Based on Sensory Indicator Assessment","volume":"13","author":"Hossain M. M.","year":"2015","journal-title":"South Asia Association for Regional Co-Operation (SAARC) Journal of Agriculture"},{"key":"e_1_2_12_5_2","volume-title":"Aquiculture Summary the State of World Fisheries and Aquaculture Report","author":"FAO","year":"2016"},{"key":"e_1_2_12_6_2","first-page":"1071","article-title":"Isolation of Aeromonas Strains from the Intestinal Flora of Atlantic Salmon","volume":"27","author":"Dugenci S. K.","year":"2003","journal-title":"Turkey Journal of Veterinary Animal Science"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/23308249.2016.1187582"},{"key":"e_1_2_12_8_2","first-page":"50","article-title":"Major fish-borne Bacterial and Parasitic Zoonoses in Ethiopia: a Review","volume":"6","author":"Sorsa M.","year":"2019","journal-title":"Int. J. Fauna Biol. Stud."},{"key":"e_1_2_12_9_2","first-page":"281","article-title":"Fish Species Diversity in the Main Drainage Systems of Ethiopia: Current State of Knowledge and Research Perspectives","volume":"5","author":"Golubtsov A. S.","year":"2003","journal-title":"Ethiopian Journal of Natural Resources"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2021.03.002"},{"key":"e_1_2_12_11_2","first-page":"756","article-title":"Antimicrobial Susceptibility of Escherichia coli Isolated From Fresh-Marketed Nile Tilapia (Oreochromis niloticus)","volume":"5","author":"Rafael D.","year":"2014","journal-title":"Journal of pathogens"},{"key":"e_1_2_12_12_2","article-title":"Evaluation and Significance of Pathogenic, EHEC O157: H7, E. coli in Fish and its Environment","volume":"21","author":"Atnur V.","year":"2018","journal-title":"Journal of Experimental Zoology India"},{"key":"e_1_2_12_13_2","unstructured":"WooP. T. K.andBrunoD. W. SeptcaemiasE. EvansJ. J. andKlesiusP. H. Fish Diseases and Disorders. Volume 3: Viral Bacterial and Fungal Infections."},{"key":"e_1_2_12_14_2","volume-title":"Fish Diseases and Disorders. Volume 3: Viral, Bacterial, and Fungal Infections","author":"Woo P. T.","year":"2003"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00700"},{"key":"e_1_2_12_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-43-67"},{"key":"e_1_2_12_17_2","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1111\/jfd.12127","article-title":"Mortality and Pathology of Hybrid Catfish, Clarias macrocephalus (Gunther), Clarias gariepinus (Burchell), Associated with E. ictaluri Infection in Southern Thailand","volume":"4","author":"Suanyuk N.","year":"2014","journal-title":"Journal of Fish Diseases"},{"key":"e_1_2_12_18_2","article-title":"Isolation And, Identification of Edwardsiella tarda from Lake Ziway and Langano, Southern Oromia, Ethiopia","volume":"7","author":"Kebede B.","year":"2016","journal-title":"Fisheries and Aquaculture Journal"},{"key":"e_1_2_12_19_2","first-page":"512","volume-title":"Fish Diseases and Disorder: Viral, Bacterial and Fungal Infections","author":"Evens J. J.","year":"2011"},{"key":"e_1_2_12_20_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12038-007-0143-8"},{"key":"e_1_2_12_21_2","first-page":"343","article-title":"A Potential Fish Sources of Bacterial Pathogens for Human Beings","volume":"49","author":"Novotony L.","year":"2004","journal-title":"International Journal of Veterinary Medicine"},{"key":"e_1_2_12_22_2","volume-title":"Biology and Culture of Channel Cat-Fish","author":"Hawke J. P.","year":"2004"},{"key":"e_1_2_12_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2005.01.002"},{"key":"e_1_2_12_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2021.149205"},{"key":"e_1_2_12_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0959-8030(94)90032-9"},{"key":"e_1_2_12_26_2","first-page":"260","article-title":"Isolation of Edwardsiella tarda like Species and its Frequency of Occurrence in Fresh Water Fish Harvested for Human Consumption from Lake Hawassa and Crater Lakes Around Bishoftu, Ethiopia","volume":"5","author":"Abayneh T.","year":"2017","journal-title":"African Journal of Fisheries Science"},{"key":"e_1_2_12_27_2","doi-asserted-by":"publisher","DOI":"10.5897\/ajmr2018.8654"},{"key":"e_1_2_12_28_2","first-page":"1","article-title":"Occurrence and Distribution of Bacterial Pathogensof Fish in the Southern Gulf of Lake Tana, Bahir Dar, Ethiopia","volume":"24","author":"Nuru A.","year":"2012","journal-title":"Livestock Research for Rural Development"},{"key":"e_1_2_12_29_2","volume-title":"Background of Mirab Abaya District, MAWLFR","author":"Mawlfr (Mirab Abaya Wereda Livestock and Fishery resource Office)","year":"2020"},{"key":"e_1_2_12_30_2","volume-title":"Gibe III Environmental and Social Impact Assessment and Water Chemical Characteristics, Ethiopian Electric Power Corporation (Eepco)","author":"EEPCo (Ethiopian Electric Power Corporation)","year":"2009"},{"key":"e_1_2_12_31_2","first-page":"85","article-title":"Federal Democratic Republic of Ethiopia, Agricultural Sample Survey","volume":"446","author":"CSA (Central statistical agency)","year":"2009","journal-title":"Statistical Bulletin"},{"key":"e_1_2_12_32_2","article-title":"System: The World Geodetic System 1984 the Universal Transverse Mercatorzone 37 N Projections: Transverse Mercator Datum","author":"Gisarc C.","year":"2022","journal-title":"World Geodetic System 1984 Unit Meter"},{"key":"e_1_2_12_33_2","volume-title":"Veterinary Epidemiology, Edinburgh Veterinary Clinical Sciences Royal (Dick) School of Veterinary Studies","author":"Thrusfeild M.","year":"2018"},{"key":"e_1_2_12_34_2","first-page":"87","article-title":"The Socioeconomics of Small Scale Fisheries Based on Eastern Side of Lake Abaya","volume":"2","author":"Samson D.","year":"2015","journal-title":"International Journal of Fisheries and Aquatic Studies"},{"key":"e_1_2_12_35_2","volume-title":"Bele Town Small Scale Enterprise Fish Producer Organization at Gilegele Gibe-3 Dam Daily Record Format","author":"Tscfpe Z.","year":"2022"},{"key":"e_1_2_12_36_2","doi-asserted-by":"publisher","DOI":"10.1590\/s0102-09352008000100040"},{"key":"e_1_2_12_37_2","volume-title":"Manual of Diagnostic Tests for Aquatic Animals","author":"OIE (Office International des Epizooties)","year":"2006"},{"key":"e_1_2_12_38_2","first-page":"681","article-title":"Pheno Typing, Geno Typing and Whole Cell Protein Proiling of E. Tarda. Isolated from Cultured and Natural Habitat Fresh Water Fish","volume":"3","author":"Wei L. S.","year":"2008","journal-title":"American Eurasian Agriculture and Environmental Science"},{"key":"e_1_2_12_39_2","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1111\/j.1365-2109.2005.01394.x","article-title":"Intra-Andinter-Specific Phenotypic Characteristics of Fish-Pathogenic E.Ictaluri and E.Tarda","volume":"37","author":"Panangala V. S.","year":"2006","journal-title":"Aquaculture Research"},{"key":"e_1_2_12_40_2","volume-title":"Isolation and Identification of Enterohamorragic E. coli O157","author":"ISO (International Organization for Standardization)","year":"2003"},{"key":"e_1_2_12_41_2","volume-title":"Microbiology of the Food Chain Horizontal Method for the Enumeration of \u03b2-Glucuronidase-Positive Escherichia coli for Microbiological Analysis","author":"ISO (International Organization for Standardization)","year":"2015"},{"key":"e_1_2_12_42_2","article-title":"National Wild Fish Health Survey-Laboratory Procedures Manual","author":"Woodland J.","year":"2006","journal-title":"Fish and Wildlife Service"},{"key":"e_1_2_12_43_2","volume-title":"Clinical Veterinary Microbiology","author":"Quinine P. J.","year":"2002"},{"key":"e_1_2_12_44_2","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing","author":"ClSI (Clinical and Laboratory Standards Institute)","year":"2015"},{"key":"e_1_2_12_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2006.01454.x"},{"key":"e_1_2_12_46_2","doi-asserted-by":"publisher","DOI":"10.1007\/bf02015051"},{"key":"e_1_2_12_47_2","first-page":"377","article-title":"Electro-Microscopy of the Intestinal Micro flora of Fish","volume":"36","author":"Ringoa E.","year":"2003","journal-title":"Journal of Aquaculture"},{"key":"e_1_2_12_48_2","first-page":"373","article-title":"Water Quality and Fresh Water Fish Diversity at Khao Luang National Park, Thailand","volume":"19","author":"Sutin S.","year":"2007","journal-title":"Journal of Transactions on Engineering, Computing and Technology"},{"key":"e_1_2_12_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2004.09.006"},{"key":"e_1_2_12_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2005.00670.x"},{"key":"e_1_2_12_51_2","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1111\/j.1095-8649.1977.tb04086.x","article-title":"A Review of the Bacterial Flora of Teleosts and Clasmo Branches Including Methods for Its Analysis","volume":"10","author":"Horsley R. W.","year":"1997","journal-title":"Journal of Fish Biology"},{"key":"e_1_2_12_52_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4172\/2150-3508.1000238","article-title":"Preliminary Survey of Gram-Negative Bacterial Pathogens from Commonly Caught Fish Species in Lake Hayiqe, Ethiopia","volume":"9","author":"Tesfaye S.","year":"2018","journal-title":"Fisheries and Aquaculture Journal"},{"key":"e_1_2_12_53_2","first-page":"260","article-title":"Isolation of Edwardsiella Tarda-like Species and Its Frequency of Occurrence in Fresh Water Fish Harvested for Human Consumption from Lake Hawassa and Crater Lakes Around Bishoftu, Ethiopia","volume":"5","author":"Nuredin N.","year":"2017","journal-title":"African Journal of Fisheries Science"},{"key":"e_1_2_12_54_2","article-title":"Isolation, Identification and Antimicrobial Susceptibility Profile of E. coli O157:H7 from Fish in Lake Hawasa","volume":"17","author":"Aynadis T.","year":"2020","journal-title":"Life Science Journal"},{"key":"e_1_2_12_55_2","doi-asserted-by":"crossref","DOI":"10.1016\/j.onehlt.2022.100447","article-title":"Cluster Analysis and Geospatial Mapping of Antibiotic-Resistant Escherichia coli O157 in Southwest Nigerian Communities","volume":"15","author":"Akinduti A. P.","year":"2022","journal-title":"One Health"},{"key":"e_1_2_12_56_2","doi-asserted-by":"publisher","DOI":"10.1093\/fqsafe\/fyz015"},{"key":"e_1_2_12_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.01551-06"},{"key":"e_1_2_12_58_2","volume-title":"Antimicrobial Resistance Global Report on Surveillance","author":"WHO (World Health Organization)","year":"2014"},{"key":"e_1_2_12_59_2","first-page":"583","article-title":"Monitoring and Surveillance of Antimicrobial Resistance in Microorganisms Associated with Aquatic Animals","volume":"32","author":"Smith V.","year":"2013","journal-title":"Journal of Science and Technology"},{"key":"e_1_2_12_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2019.e02996"},{"key":"e_1_2_12_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meatsci.2012.11.023"},{"key":"e_1_2_12_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.68.2.576-581.2002"},{"key":"e_1_2_12_63_2","volume-title":"Isolation and Antimicrobial Susceptibility Profile of Edwardsiella Species and Escherichia Coli O157:H7 from Fish Harvested for Human Consumption From Lake Abaya and Gilegel Gibe-3 Dam, Southern, Ethiopia","author":"Walelign B.","year":"2022"}],"container-title":["Veterinary Medicine International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/vmi\/4857001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/vmi\/4857001","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/vmi\/4857001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T11:34:52Z","timestamp":1791372892000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/vmi\/4857001"}},"subtitle":[],"editor":[{"given":"Lipi","family":"Buch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":63,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/vmi\/4857001"],"URL":"https:\/\/doi.org\/10.1155\/vmi\/4857001","archive":["Portico"],"relation":{},"ISSN":["2090-8113","2042-0048"],"issn-type":[{"value":"2090-8113","type":"print"},{"value":"2042-0048","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-05-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"4857001"}}