{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T05:57:20Z","timestamp":1781762240137,"version":"3.54.5"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T00:00:00Z","timestamp":1781654400000},"content-version":"vor","delay-in-days":167,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Enhancement of the production of eggs with good\u2010quality eggshells is of serious interest to ensure food security as eggs are assessed mostly by their eggshell quality. A high rate of cracked eggs has been reported to cause economic loss, increasing the risk of bacterial infections leading to low egg production. Hence, the study was conducted to determine the genetic variation of the\n                    <jats:italic>Sodium Channel Epithelial 1 Subunit Gamma<\/jats:italic>\n                    (\n                    <jats:italic>SCNN1G<\/jats:italic>\n                    ) gene in improving eggshell thickness of the Potchefstroom Koekoek chicken breed. The study was conducted at the University of Limpopo with 100 Potchefstroom Koekoek point\u2010of\u2010lay chickens raised intensively. A total of 350 eggs were randomly collected at the 31\n                    <jats:sup>st<\/jats:sup>\n                    to 39\n                    <jats:sup>th<\/jats:sup>\n                    weeks for the collection of internal, external, and eggshell quality traits, and tissues (shell gland, magnum, and isthmus) and blood samples were collected for molecular analysis. Statistical Analysis System Version 9.4 (SAS, 2020) software was used for data analysis. DNA sequencing, general linear model (GLM), and quantitative reverse transcription PCR (RT\u2010qPCR) were used to achieve the objective. DNA sequence analysis revealed two SNPs (A &gt; T at position 2587 and C &gt; T at position 1013 in the coding region). Marker\u2013trait association showed shell thickness (ST) having a significant association (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05) with all the genotypes where genotype AA was associated with thicker ST than genotype AT; then, genotype CC was associated with thicker ST than genotype CT. The RT\u2010qPCR results demonstrated significant difference (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05) in\n                    <jats:italic>SCNN1G<\/jats:italic>\n                    mRNA expression levels where the highest expression level was observed in magnum, followed by isthmus and shell gland, respectively. The findings of the study could give evidence on the relevance of genetic markers, mRNA expression analysis, and other molecular biology tools that could be used during genetic selection programs aimed at improving egg production traits specifically eggshell quality.\n                  <\/jats:p>","DOI":"10.1155\/vmi\/5917044","type":"journal-article","created":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T05:42:42Z","timestamp":1781761362000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Expression Profile of the\n                    <i>SCNN1G<\/i>\n                    Gene and Its Association of Polymorphisms With Eggshell Quality Traits in the Potchefstroom Koekoek Chicken Breed"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6608-1523","authenticated-orcid":false,"given":"Kagisho Madikadike","family":"Molabe","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3519-7806","authenticated-orcid":false,"given":"Thobela Louis","family":"Tyasi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5212-1347","authenticated-orcid":false,"given":"Vusi","family":"Mbazima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,17]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1080\/10495398.2016.1169191"},{"key":"e_1_2_9_2_2","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.ecss.2013.10.024","article-title":"Variability in the Egg Production Rates of the Calanoid Copepod, Pseudodiaptomus Hessei in a South African Estuary in Relation to Environmental Factors","volume":"135","author":"Noyon M.","year":"2013","journal-title":"Estuarine, Coastal and Shelf Science"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.48550\/arXiv.2201.01821"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-29162-7"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1080\/00439339.2020.1866965"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1017\/S2078633610000664"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.3382\/ps.2010-00888"},{"key":"e_1_2_9_8_2","volume-title":"Assessing the Husbandry Practices, Productivity and Egg Quality Traits of Local and Sasso Chickens in Two Agro Ecologies of Daro Lebu District, West Hararghe, Ethiopia","author":"Yusuf A. J.","year":"2021"},{"key":"e_1_2_9_9_2","first-page":"51","article-title":"Polymorphism of the SCNN1 G Gene and Its Association With Eggshell Quality","volume":"5","author":"Kheirkhah Z.","year":"2017","journal-title":"Poultry Science"},{"key":"e_1_2_9_10_2","doi-asserted-by":"crossref","first-page":"245","DOI":"10.1533\/9780857093912.3.245","volume-title":"Improving the Safety and Quality of Eggs and Egg Products","author":"Dunn I.","year":"2011"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/850170"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.22372"},{"key":"e_1_2_9_13_2","first-page":"205","article-title":"Sequencing of Growth Hormone Gene for Detection of Polymorphisms and Their Relationship With Body Weight in Harri Sheep","volume":"51","author":"Abdelmoneim T. S.","year":"2017","journal-title":"Indian Journal of Animal Research"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1080\/00071668.2020.1817329"},{"key":"e_1_2_9_15_2","first-page":"880","article-title":"New Polymorphisms of PAPPA and PAPPA2 Genes and Their Associations With Egg Production Traits in Chinese Dagu Chickens","volume":"53","author":"Liu D.","year":"2017","journal-title":"Indian Journal of Animal Research"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-819770-7.00049-9"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.869149"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.3923\/ijps.2013.245.250"},{"key":"e_1_2_9_19_2","first-page":"331","article-title":"Influence of Egg Weight on Egg Quality Parameters and Growth Traits in Ring Necked Pheasants in Captivity","volume":"26","author":"Ashraf S.","year":"2016","journal-title":"Journal of Animal and Plant Sciences"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2156-14-90"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2052.2008.01794.x"}],"container-title":["Veterinary Medicine International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/vmi\/5917044","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/vmi\/5917044","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/vmi\/5917044","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T05:42:44Z","timestamp":1781761364000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/vmi\/5917044"}},"subtitle":[],"editor":[{"given":"Valeria","family":"Pasciu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/vmi\/5917044"],"URL":"https:\/\/doi.org\/10.1155\/vmi\/5917044","archive":["Portico"],"relation":{},"ISSN":["2090-8113","2042-0048"],"issn-type":[{"value":"2090-8113","type":"print"},{"value":"2042-0048","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2026-01-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"5917044"}}