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This study aimed to determine the prevalence of\n                    <jats:italic>Anaplasma<\/jats:italic>\n                    spp. and hemotropic\n                    <jats:italic>Mycoplasma<\/jats:italic>\n                    spp. in goats in Mazandaran Province, Northern Iran. A total of 149 blood samples were collected from goats of different ages and sexes during the spring season. The samples were analyzed using polymerase chain reaction (PCR) to identify\n                    <jats:italic>Anaplasma<\/jats:italic>\n                    spp. and\n                    <jats:italic>Mycoplasma<\/jats:italic>\n                    spp. To identify bacterial species, representative amplicons of\n                    <jats:italic>Mycoplasma<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20094) and\n                    <jats:italic>Anaplasma<\/jats:italic>\n                    (\n                    <jats:italic>n<\/jats:italic>\n                    \u2009=\u20096) were sequenced. PCR testing revealed a prevalence of 19.4% for\n                    <jats:italic>Anaplasma<\/jats:italic>\n                    spp. and 28.1% for hemotropic\n                    <jats:italic>Mycoplasma<\/jats:italic>\n                    . Statistical analysis of blood parameters indicated significant differences between the\n                    <jats:italic>Anaplasma<\/jats:italic>\n                    \u2010positive and negative groups, with a notable decrease in red blood cell (RBC) count and hematocrit (HCT) levels, while alkaline phosphatase (ALP) and triglyceride levels showed significant increases. In contrast, no significant differences were observed in hematological and biochemical parameters for the\n                    <jats:italic>Mycoplasma<\/jats:italic>\n                    \u2010positive group. Molecular and phylogenetic analyses confirmed the presence of\n                    <jats:italic>M. ovis<\/jats:italic>\n                    (PQ793282),\n                    <jats:italic>Anaplasma capra<\/jats:italic>\n                    (PV176424),\n                    <jats:italic>Anaplasma ovis<\/jats:italic>\n                    (PV185359), and\n                    <jats:italic>Anaplasma phagocytophilum<\/jats:italic>\n                    (PV175399) in Iranian goats. This research represents the first report of\n                    <jats:italic>M. ovis<\/jats:italic>\n                    and\n                    <jats:italic>A. capra<\/jats:italic>\n                    in Iran and highlights the need for enhanced molecular surveillance of\n                    <jats:italic>Anaplasma<\/jats:italic>\n                    and\n                    <jats:italic>Mycoplasma<\/jats:italic>\n                    infections in livestock. Consequently, these pathogens\u2014especially those exhibiting close genetic relationships with human pathogens\u2014represent significant threats to both livestock and human health. This underscores the need for enhanced surveillance, recognition of potential zoonotic risks, vector control strategies, and vaccination measures.\n                  <\/jats:p>","DOI":"10.1155\/vmi\/5985068","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T19:35:47Z","timestamp":1782848147000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Diagnosis and Phylogenetic Analysis of Hemotropic Mycoplasmas and\n                    <i>Anaplasma<\/i>\n                    spp. in Peripheral Blood of Goats in Northern Iran and Their Hematobiochemical Alterations"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2341-2150","authenticated-orcid":false,"given":"Masoud","family":"Nematinezhad","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1243-5744","authenticated-orcid":false,"given":"Hassan","family":"Sharifiyazdi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5501-1028","authenticated-orcid":false,"given":"Saeed","family":"Nazifi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4286-749X","authenticated-orcid":false,"given":"Tina","family":"Yaghoobpour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mojtaba","family":"Rahsepar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyed Mohammad Bagher","family":"Hosseini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"e_1_2_12_1_2","volume-title":"Veterinary Medicine: A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs and Goats","author":"Radostits O. 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