{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T07:21:52Z","timestamp":1787556112934,"version":"build-2736575974"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T00:00:00Z","timestamp":1787443200000},"content-version":"vor","delay-in-days":234,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["155\/2021"],"award-info":[{"award-number":["155\/2021"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    The Alphaherpesvirinae subfamily belongs to the Orthoherpesviridae family, whose viruses are characterized by rapid replication and the ability to establish latent infection in host cells. These viruses cause important avian diseases such as Marek\u2019s disease, infectious laryngotracheitis, and Pacheco\u2019s disease (PD) in psittacines. In Brazilian avifauna, especially within the Psittacidae family, sanitary problems are frequently observed due to stress caused by deforestation and wildlife trafficking, factors that can exacerbate herpesvirus infections. Due to the limited available data on herpesvirus occurrence in wild psittacines, the aim of this study was to survey and identify alphaherpesviruses in psittacine species in Mato Grosso using the polymerase chain reaction (PCR) technique, correlating the results with phylogenetic analysis. Biological samples were obtained from IBAMA, SEMA, and CEMPAS and sent to the Veterinary Hospital of UFMT (HOVET) between 2019 and 2024. PCR\u2010positive samples, which amplified a 264\u2010bp fragment of the UL30 gene, were subjected to sequencing and phylogenetic analysis. In total, 131 samples were analyzed, including liver tissue, cloacal swabs, and blood, from both captive and free\u2010living birds. Twelve (9.16%) were positive by PCR, with swabs showing the highest detection rate. A sequenced sample from\n                    <jats:italic>Amazona aestiva<\/jats:italic>\n                    showed 79.77% amino acid identity with\n                    <jats:italic>Eupsittula herpesvirus<\/jats:italic>\n                    , suggesting a potentially distinct alphaherpesvirus lineage. Another positive sample from\n                    <jats:italic>Ara ararauna<\/jats:italic>\n                    showed 97.65% identity with\n                    <jats:italic>Meleagrid alphaherpesvirus 1<\/jats:italic>\n                    . These results demonstrate the detection of alphaherpesviruses in psittacine birds from Mato Grosso and reveal phylogenetic relationships that warrant further genomic investigation to better understand viral diversity and potential host associations.\n                  <\/jats:p>","DOI":"10.1155\/vmi\/6417751","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T06:57:36Z","timestamp":1787554656000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Occurrence and Molecular Characterization of Alphaherpesvirus in Psittacines in the State of Mato Grosso"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-0456-7934","authenticated-orcid":false,"given":"Thais Rosso da","family":"Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7720-818X","authenticated-orcid":false,"given":"Marco T\u00falio dos Santos","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4676-1094","authenticated-orcid":false,"given":"Danny Franciele da Silva Dias","family":"Moraes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7902-7833","authenticated-orcid":false,"given":"Camila Regina Almeida","family":"Vani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-1757-3099","authenticated-orcid":false,"given":"Augusto Cesar dos Santos","family":"Stering","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6630-2293","authenticated-orcid":false,"given":"Val\u00e9ria","family":"Dutra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1244-0690","authenticated-orcid":false,"given":"Luciano","family":"Nakazato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,23]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.001673"},{"key":"e_1_2_9_2_2","doi-asserted-by":"crossref","first-page":"32","DOI":"10.17582\/journal.hv\/2022\/9.32.37","article-title":"Pacheco\u2019s Disease Outbreak With High Mortality in Brazilian Captive Psittacine Birds","volume":"9","author":"Barbosa C. M.","year":"2022","journal-title":"Hosts and Viruses"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.06.027"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-023-04605-w"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00134-06"},{"key":"e_1_2_9_6_2","unstructured":"Iucn Red List IUCN Red List of Threatened Species 2024 http:\/\/www.iucnredlist.org."},{"key":"e_1_2_9_7_2","volume-title":"Red Book of Brazilian Fauna Threatened with Extinction: Volume III\u2013Birds","author":"Instituto Chico Mendes de Conserva\u00e7\u00e3o da Biodiversidade (Icmbio)","year":"2018"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjm.2015.11.017"},{"key":"e_1_2_9_9_2","volume-title":"Molecular Detection of Psittacine Beak and Feather Disease Virus and Chlamydia psittaci in Wild and Captive Birds in the State of Mato Grosso","author":"Rocha I. S. M.","year":"2023"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.12.5463"},{"key":"e_1_2_9_11_2","volume-title":"Diseases Diagnosed in Wild and Exotic Birds at the Pathology Department of the Veterinary School of the Federal University of Minas Gerais From 2006 to 2021","author":"Lacerda M. S. C.","year":"2022"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.822212"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1055-937x(05)80041-6"},{"key":"e_1_2_9_14_2","first-page":"1","article-title":"Doen\u00e7a de Pacheco: Um Mal Silencioso Para Psitac\u00eddeos","author":"Reis L. W. D.","year":"2023","journal-title":"Arquivos de Ci\u00eancias Veterin\u00e1rias e Zool\u00f3gicas da UNIPAR (Online)"},{"key":"e_1_2_9_15_2","volume-title":"Molecular Characterization of Meleagrid Alphaherpesvirus 1","author":"Mahony T. J.","year":"2015"}],"container-title":["Veterinary Medicine International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/vmi\/6417751","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/vmi\/6417751","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/vmi\/6417751","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T06:57:38Z","timestamp":1787554658000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/vmi\/6417751"}},"subtitle":[],"editor":[{"given":"Lipi","family":"Buch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/vmi\/6417751"],"URL":"https:\/\/doi.org\/10.1155\/vmi\/6417751","archive":["Portico"],"relation":{},"ISSN":["2090-8113","2042-0048"],"issn-type":[{"value":"2090-8113","type":"print"},{"value":"2042-0048","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2026-02-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"6417751"}}