{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T10:15:28Z","timestamp":1788084928081,"version":"build-2784847793"},"reference-count":83,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"vor","delay-in-days":241,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004434","name":"Universit\u00e0 degli Studi di Firenze","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004434","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Aging in dogs mirrors key biological aspects of human aging, including molecular processes associated with neurodegeneration, making the dog a valuable comparative model. This pilot study investigated the integration of circulating blood biomarkers and genomic architecture to explore interindividual variability associated with neurobiological aging in dogs. Forty\u2010eight dogs (31 purebred Labrador Retrievers and 17 mixed\u2010breed dogs; age range: 2\u201315\u2009years) were analyzed for serum phosphorylated tau at threonine 181 (pTau181). Genome\u2010wide SNP genotyping was performed to characterize population structure, estimate pedigree\u2010 and ROH\u2010based genomic inbreeding, and conduct an exploratory genome\u2010wide association study (GWAS). Circulating pTau181 produced a quantifiable signal, but a subset of values fell below the assay\u2019s lower limit of detection and were coded as zero for statistical analysis. Data showed a continuous distribution, with mean values ranging from 6.1 to 13.4\u2009pg\/mL across demographic groups. No significant associations were observed between pTau181 concentrations and age category, sex, or breed group. Genomic analyses revealed clear population stratification between Labrador Retrievers and mixed\u2010breed dogs, with significantly higher genomic homozygosity in Labradors (FROH\u2009=\u20090.26\u2009\u00b1\u20090.05) compared with mixed breeds (FROH\u2009=\u20090.12\u2009\u00b1\u20090.14;\n                    <jats:italic>p<\/jats:italic>\n                    = 0.00025). No significant correlations were detected between circulating pTau181 levels and genomic inbreeding coefficients. Exploratory GWAS identified a suggestive association between pTau181 concentrations and a locus on chromosome 29 (top SNP\n                    <jats:italic>p<\/jats:italic>\n                    = 5.57 \u00d7 10\n                    <jats:sup>\u22127<\/jats:sup>\n                    ), encompassing genes involved in proteostasis, neurodevelopment, and cellular metabolism. Although these associations did not reach genome\u2010wide significance, they point to a biologically coherent genomic region potentially contributing to interindividual variability in tau blood levels. Overall, these findings provide preliminary evidence supporting the feasibility of combining circulating pTau181 measurements with genome\u2010wide analyses to investigate molecular and genetic aspects of neurobiological aging in dogs within a comparative research framework.\n                  <\/jats:p>","DOI":"10.1155\/vmi\/7159552","type":"journal-article","created":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T10:08:58Z","timestamp":1788084538000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Circulating pTau181 and Genomic Background in Dogs: A Pilot Study on Neurobiological Aging"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-2021-3082","authenticated-orcid":false,"given":"Valentina","family":"Gazzano","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3224-745X","authenticated-orcid":false,"given":"Maria Chiara","family":"Fabbri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2670-8237","authenticated-orcid":false,"given":"Simona","family":"Capsoni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0141-6449","authenticated-orcid":false,"given":"Carlo","family":"Cantile","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0535-1155","authenticated-orcid":false,"given":"Lara","family":"Tinacci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9649-2027","authenticated-orcid":false,"given":"Angelo","family":"Gazzano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7342-6411","authenticated-orcid":false,"given":"Maria Claudia","family":"Curadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5483-6354","authenticated-orcid":false,"given":"Francesca","family":"Cecchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,30]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.4454\/db.v9i2.169"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani14213073"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-animal-051021-080937"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-04282-9"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-15837-9"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.20506\/rst.42.3349"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2023.1139308"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-025-01749-9"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41398-024-02898-9"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2025.102971"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/cm.21822"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-009-0486-3"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fncel.2018.00338"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1101\/2021.08.21.456385"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2021.767322"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/bpa.12996"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2019.01.007"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2021.03.003"},{"key":"e_1_2_11_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2005.02.009"},{"key":"e_1_2_11_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41380-024-02501-z"},{"key":"e_1_2_11_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.25396"},{"key":"e_1_2_11_22_2","doi-asserted-by":"publisher","DOI":"10.3390\/antiox14070774"},{"key":"e_1_2_11_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-56469-7"},{"key":"e_1_2_11_24_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24031869"},{"key":"e_1_2_11_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40659-025-00607-4"},{"key":"e_1_2_11_26_2","doi-asserted-by":"publisher","DOI":"10.15252\/emmm.202012921"},{"key":"e_1_2_11_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1474-4422(20)30071-5"},{"key":"e_1_2_11_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/bs.acc.2023.05.001"},{"key":"e_1_2_11_29_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-022-00586-0"},{"key":"e_1_2_11_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-54878-8"},{"key":"e_1_2_11_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0781-z"},{"key":"e_1_2_11_32_2","doi-asserted-by":"publisher","DOI":"10.1001\/jamaneurol.2020.4986"},{"key":"e_1_2_11_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13024-023-00605-8"},{"key":"e_1_2_11_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/bpa.12893"},{"key":"e_1_2_11_35_2","doi-asserted-by":"publisher","DOI":"10.21873\/invivo.13181"},{"key":"e_1_2_11_36_2","doi-asserted-by":"publisher","DOI":"10.4454\/db.v10i3.196"},{"key":"e_1_2_11_37_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-022-00665-2"},{"key":"e_1_2_11_38_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0328470"},{"key":"e_1_2_11_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-022-01024-z"},{"key":"e_1_2_11_40_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41588-019-0358-2"},{"key":"e_1_2_11_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.24454"},{"key":"e_1_2_11_42_2","doi-asserted-by":"publisher","DOI":"10.1002\/alz.12191"},{"key":"e_1_2_11_43_2","doi-asserted-by":"publisher","DOI":"10.3390\/genes16121463"},{"key":"e_1_2_11_44_2","doi-asserted-by":"publisher","DOI":"10.1101\/2025.05.21.25328076"},{"key":"e_1_2_11_45_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-021-09902-6"},{"key":"e_1_2_11_46_2","doi-asserted-by":"publisher","DOI":"10.4081\/ijas.2013.e54"},{"key":"e_1_2_11_47_2","doi-asserted-by":"crossref","DOI":"10.1007\/s12210-020-00914-w","article-title":"Inbreeding, Phenotypic Traits, Coat Colours and Prevalence of Health Problems in a Population of English Cocker Spaniels: The First Survey in Italy","volume":"31","author":"Cecchi F.","year":"2020","journal-title":"Rendiconti Lincei. Scienze Fisiche e Naturali"},{"key":"e_1_2_11_48_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40575-021-00111-4"},{"key":"e_1_2_11_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.livsci.2014.05.034"},{"key":"e_1_2_11_50_2","unstructured":"SargolzaeiM. IwaisakiH. andColleauJ. J. CFC (Contribution Inbreeding (F) Coancestry) Proceedings of the Proceedings of the 8th World Congress on Genetics Applied to Livestock Production 2006 Belo Horizonte Brazil."},{"key":"e_1_2_11_51_2","doi-asserted-by":"publisher","DOI":"10.1086\/279872"},{"key":"e_1_2_11_52_2","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9686-24-4-305"},{"key":"e_1_2_11_53_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13742-015-0047-8"},{"key":"e_1_2_11_54_2","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1007\/978-3-319-24277-4_9","volume-title":"ggplot2: Elegant Graphics for Data Analysis","author":"Wickham H.","year":"2016"},{"key":"e_1_2_11_55_2","doi-asserted-by":"publisher","DOI":"10.21105\/joss.00731"},{"key":"e_1_2_11_56_2","doi-asserted-by":"crossref","unstructured":"BiscariniF. CozziP. GaspaG. andMarrasG. DetectRUNS: Detect Runs of Homozygosity and Runs of Heterozygosity in Diploid Genomes 2018.","DOI":"10.32614\/CRAN.package.detectRUNS"},{"key":"e_1_2_11_57_2","doi-asserted-by":"publisher","DOI":"10.1111\/age.12259"},{"key":"e_1_2_11_58_2","unstructured":"WeiT. SimkoV. LevyM.et al. Corrplot: Visualization of a Correlation Matrix 2024."},{"key":"e_1_2_11_59_2","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2020.595532"},{"key":"e_1_2_11_60_2","doi-asserted-by":"crossref","first-page":"217","DOI":"10.3103\/S009545271304004X","article-title":"Investigation on the Genetic Variability of the American Pit Bull Terrier Dogs","volume":"47","author":"Ciampolini R.","year":"2013","journal-title":"Cytology and Genetics"},{"key":"e_1_2_11_61_2","doi-asserted-by":"publisher","DOI":"10.1080\/1828051x.2015.1128689"},{"key":"e_1_2_11_62_2","unstructured":"BeuchatC. COI FAQs: Understanding the Coefficient of Inbreeding 2020."},{"key":"e_1_2_11_63_2","doi-asserted-by":"crossref","DOI":"10.3390\/ani11020269","article-title":"Discriminant Canonical Analysis of the Contribution of Spanish and Arabian Purebred Horses","volume":"11","author":"Mar\u00edn Navas C.","year":"2021","journal-title":"Animals"},{"key":"e_1_2_11_64_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12210-018-0676-9"},{"key":"e_1_2_11_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.03.079"},{"key":"e_1_2_11_66_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1516215112"},{"key":"e_1_2_11_67_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1512501113"},{"key":"e_1_2_11_68_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12862-015-0579-7"},{"key":"e_1_2_11_69_2","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1534\/g3.118.200836","article-title":"Fine-Scale Resolution of Runs of Homozygosity Reveal Patterns of Inbreeding","volume":"9","author":"Sams A. J.","year":"2019","journal-title":"G3: Genes, Genomes, Genetics"},{"key":"e_1_2_11_70_2","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1038\/nrg.2017.109","article-title":"Runs of Homozygosity: Windows Into Population History and Trait Architecture","volume":"19","author":"Ceballos F. C.","year":"2018","journal-title":"Nature Reviews Genetics"},{"key":"e_1_2_11_71_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-017-3933-x"},{"key":"e_1_2_11_72_2","doi-asserted-by":"crossref","DOI":"10.3390\/ani10030498","article-title":"The German Shorthair Pointer Dog Breed: Genomic Inbreeding and Variability","volume":"10","author":"Boccardo A.","year":"2020","journal-title":"Animals"},{"key":"e_1_2_11_73_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2019116118"},{"key":"e_1_2_11_74_2","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1007\/s13353-022-00693-0","article-title":"No Evidence That Long Runs of Homozygosity Tend to Harbor Risk Variants","volume":"63","author":"Szydlowski M.","year":"2022","journal-title":"Journal of Applied Genetics"},{"key":"e_1_2_11_75_2","doi-asserted-by":"publisher","DOI":"10.3389\/fnagi.2016.00303"},{"key":"e_1_2_11_76_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-025-05220-w"},{"key":"e_1_2_11_77_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-06380-0"},{"key":"e_1_2_11_78_2","doi-asserted-by":"publisher","DOI":"10.3390\/biom14030255"},{"key":"e_1_2_11_79_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.551895"},{"key":"e_1_2_11_80_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jveb.2026.04.011"},{"key":"e_1_2_11_81_2","doi-asserted-by":"publisher","DOI":"10.4454\/db.v9i3.178"},{"key":"e_1_2_11_82_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-020-02195-x"},{"key":"e_1_2_11_83_2","doi-asserted-by":"publisher","DOI":"10.1093\/braincomms\/fcab136"}],"container-title":["Veterinary Medicine International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/vmi\/7159552","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/vmi\/7159552","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/vmi\/7159552","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T10:09:02Z","timestamp":1788084542000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/vmi\/7159552"}},"subtitle":[],"editor":[{"given":"Lipi","family":"Buch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":83,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/vmi\/7159552"],"URL":"https:\/\/doi.org\/10.1155\/vmi\/7159552","archive":["Portico"],"relation":{},"ISSN":["2090-8113","2042-0048"],"issn-type":[{"value":"2090-8113","type":"print"},{"value":"2042-0048","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2026-03-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"7159552"}}