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This study evaluated the immunological profile induced by a mixture of recombinant antigens, formulated with cationic liposomes containing different combinations of\n                    <jats:italic>gemini<\/jats:italic>\n                    lipopeptide AG2\u2010C16, bovine\u2010specific CpG oligodeoxynucleotide, and\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    mannans, in dairy heifers. Thirty\u2010five dairy heifers aged 8\u201310\u2009months were randomly assigned to seven groups and immunized with recombinant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    antigens (\u03b1\u2010toxin, \u03b2\u2010toxin, clumping factor A, and fibronectin\u2010binding protein A) formulated with different adjuvant combinations. Animals received two primary doses 21\u2009days apart, followed by a booster dose 8\u2009months later. Humoral immune responses were assessed through ELISA measuring specific immunoglobulin G (IgG), IgG1, and IgG2 levels, while cytokine production was evaluated via whole\u2010blood assays. Results demonstrate that liposomal formulations combining AG2\u2010C16, CpG\u2010ODN, and mannans induce a significant immune response, in heifers, with IFN\u2010\u03b3 production. These findings provide valuable insights for the development of new vaccine strategies in cattle.\n                  <\/jats:p>","DOI":"10.1155\/vmi\/7845041","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:55:03Z","timestamp":1790232903000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Novel Liposomal Adjuvants Enhance the Immune Response to Recombinant\n                    <i>Staphylococcus aureus<\/i>\n                    Antigens in Dairy 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