{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T05:43:53Z","timestamp":1782798233533,"version":"3.54.5"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T00:00:00Z","timestamp":1782691200000},"content-version":"vor","delay-in-days":179,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["313101\/2023-0"],"award-info":[{"award-number":["313101\/2023-0"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                      represents a critical antimicrobial resistance (AMR) threat in veterinary medicine, with carbapenemase\u2010producing isolates posing significant challenges to both animal and human health. The emergence of metallo\u2010beta\u2010lactamases (MBLs) and serine carbapenemases in veterinary settings necessitates comprehensive surveillance to monitor resistance gene dissemination.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study aimed to conduct genotypic surveillance of carbapenemase\u2010encoding genes (bla\n                      <jats:sub>KPC-2<\/jats:sub>\n                      , bla\n                      <jats:sub>SPM-1<\/jats:sub>\n                      , bla\n                      <jats:sub>NDM-1<\/jats:sub>\n                      , bla\n                      <jats:sub>VIM-2<\/jats:sub>\n                      , and bla\n                      <jats:sub>IMP-1<\/jats:sub>\n                      ) in\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      isolates from domestic animals in Brazil\u2019s Midwest region, providing critical epidemiological data for antimicrobial stewardship programs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A total of 83\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      isolates were collected from various domestic animal species (57 canines, 12 bovines, 7 felines, 2 equines, 2 ovines, 2 avians, and 1 rabbit) between 2017 and 2021. Molecular identification was confirmed via 16S rRNA gene sequencing, and carbapenemase genes were detected using conventional multiplex PCR. Data were analyzed using descriptive statistics.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Carbapenemase genes were detected in 40.96% (34\/83) of isolates. The most prevalent gene was bla\n                      <jats:sub>KPC-2<\/jats:sub>\n                      at 34.9% (29\/83), followed by bla\n                      <jats:sub>SPM-1<\/jats:sub>\n                      (9.6%, 8\/83), bla\n                      <jats:sub>NDM-1<\/jats:sub>\n                      (8.4%, 7\/83), bla\n                      <jats:sub>VIM-2<\/jats:sub>\n                      (3.6%, 3\/83), and bla\n                      <jats:sub>IMP-1<\/jats:sub>\n                      (1.2%, 1\/83). Ear infections were the predominant source (53%, 44\/83), particularly in canines. Co\u2010occurrence of multiple resistance genes was observed in 7.2% of isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study reveals an alarming prevalence of carbapenemase\u2010producing\n                      <jats:italic>P. aeruginosa<\/jats:italic>\n                      in domestic animals, with bla\n                      <jats:sub>KPC-2<\/jats:sub>\n                      frequencies comparable to those in wild animal populations. The predominance of ear\u2010derived isolates suggests a potential for community\u2010acquired transmission. These findings underscore the critical need for robust antimicrobial stewardship and integrated One Health surveillance systems to monitor resistance gene flow between animal and human populations.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/vmi\/8246667","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T04:54:01Z","timestamp":1782795241000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Carbapenemase Genes Surveillance in\n                    <i>Pseudomonas aeruginosa<\/i>\n                    From Domestic Animals: A Five\u2010Year Study From Brazil\u2019s Midwest Region"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6206-039X","authenticated-orcid":false,"given":"Mayara Lima","family":"Kawasaki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3681-1430","authenticated-orcid":false,"given":"Jackeliny dos Santos","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1831-7720","authenticated-orcid":false,"given":"Ester Santa","family":"Vieira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7741-2454","authenticated-orcid":false,"given":"Anna Caroline Barbosa Alves","family":"Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila Regina Alves","family":"Vani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2362-1383","authenticated-orcid":false,"given":"Ana Carolina Lemos","family":"Caldeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0129-9037","authenticated-orcid":false,"given":"Ana Rebecca Maia","family":"Araujo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1244-0690","authenticated-orcid":false,"given":"Luciano","family":"Nakazato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6630-2293","authenticated-orcid":false,"given":"Valeria","family":"Dutra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,29]]},"reference":[{"key":"e_1_2_9_1_2","volume-title":"WHO Publishes List of Bacteria for Which New Antibiotics are Urgently Needed","author":"World Health Organization","year":"2017"},{"key":"e_1_2_9_2_2","article-title":"Dissemination of Antimicrobial Resistance in the Context of One Health: A Brief Review","volume":"56","author":"Silva L.","year":"2024","journal-title":"Brazilian Journal of Clinical Analysis"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2021.03.012"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2024.04.005"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines10071100"},{"key":"e_1_2_9_6_2","volume-title":"Epidemiological Bulletin\u2014Volume 55\u2014No. 02. Carbapenem-Resistant Microorganisms and Their Distribution in Brazil, 2015 to 2022","author":"Brazil Ministry of Health","year":"2024"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics10101234"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2023.106788"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/mrm.0000000000000349"},{"key":"e_1_2_9_10_2","volume-title":"Veterinary Microbiology and Microbial Disease","author":"Quinn P. J.","year":"2013"},{"key":"e_1_2_9_11_2","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook J.","year":"2004"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.45.4.1151-1161.2001"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1590\/s1807-59322010000900002"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12010076"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/aac.45.8.2224-2228.2001"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci9050208"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/vetsci11120656"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s42770-023-01143-7"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12040658"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2024.2311213"}],"container-title":["Veterinary Medicine International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/vmi\/8246667","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/vmi\/8246667","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/vmi\/8246667","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T04:54:04Z","timestamp":1782795244000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/vmi\/8246667"}},"subtitle":[],"editor":[{"given":"Nora","family":"Mestorino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/vmi\/8246667"],"URL":"https:\/\/doi.org\/10.1155\/vmi\/8246667","archive":["Portico"],"relation":{},"ISSN":["2090-8113","2042-0048"],"issn-type":[{"value":"2090-8113","type":"print"},{"value":"2042-0048","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-09-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"8246667"}}