{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T07:43:03Z","timestamp":1777275783136,"version":"3.51.4"},"reference-count":55,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,4,26]],"date-time":"2026-04-26T00:00:00Z","timestamp":1777161600000},"content-version":"vor","delay-in-days":115,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine International"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Peste des petits ruminants (PPR) is a severe, highly contagious, and fatal viral disease of small ruminants that causes significant production losses and mortality in Ethiopia. A cross\u2010sectional study was conducted from December 2021 to July 2022 to determine the seroprevalence of the disease in the study area. Samples were collected from the outbreak to conduct viral isolation and molecular detection, focusing specifically on sheep and goats as the study animals in the investigation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>Clinical specimens were first collected from small ruminants showing signs suggestive of PPR. Paired swab samples and postmortem tissue specimens were obtained for virus isolation and molecular characterization. During necropsy, representative tissue sections were aseptically trimmed and processed. Of 250 clinically suspected animals, 25 were selected for detailed virus isolation and molecular detection of PPR virus (PPRV). PCR\u2010positive samples were further confirmed through virus isolation in cell culture. Subsequently, blood samples (4\u2009mL) were collected from the jugular vein of 384 small ruminants older than 6 months for serological analysis. Serum was separated and analyzed to determine the seroprevalence of the infection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      PPRV was detected in 10 of 25 samples (40%)using reverse transcriptase PCR (RT\u2013PCR), and of these RT\u2013PCR\u2010positive samples, PPRV was isolated from four samples using Vero cells. The overall seroprevalence of PPRV was 20.6% (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009384). Age, herd composition, and history of the recent introduction of new small ruminants were significantly associated with seropositivity of PPR (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The study confirmed widespread circulation of PPRV in the study areas, significantly affecting small ruminant productivity. High seroprevalence indicated endemicity, with identified risk factors including introducing new small ruminants and herd composition. Recommendations emphasize integrating small ruminants with other livestock and supporting government efforts. Caution is needed due to regional variations, highlighting the importance of further molecular characterization of circulating PPRV strains.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/vmi\/9956146","type":"journal-article","created":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T06:49:28Z","timestamp":1777272568000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Detection and Seroprevalence Study of Peste des Petits Ruminants Virus in Small Ruminants in Central Ethiopia"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-1638-9419","authenticated-orcid":false,"given":"Teshome","family":"Yonas","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5352-5922","authenticated-orcid":false,"given":"Mishamo","family":"Sulayeman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2042-0761","authenticated-orcid":false,"given":"Kassaye","family":"Adamu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,26]]},"reference":[{"key":"e_1_2_15_1_2","unstructured":"World Bank International Development Association Project Appraisal Document on a Proposed Credit for a Livestock and Fisheries Sector Development Project (Project Appraisal Document No. PAD2396) 2017."},{"key":"e_1_2_15_2_2","volume-title":"Agricultural Sample Survey 2019\/20: Volume II Report on Livestock and Livestock Characteristics","author":"CSA","year":"2020"},{"key":"e_1_2_15_3_2","first-page":"433","article-title":"Prevalence of Small Ruminant Ectoparasites and Associated Risk Factors in Selected Districts of Tigray Region, Ethiopia","volume":"7","author":"Abebe R.","year":"2012","journal-title":"Global Veterinaria"},{"key":"e_1_2_15_4_2","doi-asserted-by":"publisher","DOI":"10.1186\/1746-6148-4-34"},{"key":"e_1_2_15_5_2","doi-asserted-by":"publisher","DOI":"10.17582\/journal"},{"key":"e_1_2_15_6_2","volume-title":"Veterinary Medicine: A Textbook of the Diseases of Cattle, Horses, Sheep, Pigs and Goats","author":"Constable P. 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Antigen and Molecular Detection of Peste Des Petits Ruminants Virus from Disease Outbreak Cases in Sheep and Goats in Assosa Zone Benishangul-Gumuz Region Ethiopia 2020 1\u201389 Master of Science in Veterinary Microbiology Addis Ababa University College of Veterinary Medicine and Agriculture Thesis."},{"key":"e_1_2_15_34_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-019-1828-6"},{"key":"e_1_2_15_35_2","doi-asserted-by":"publisher","DOI":"10.3390\/ani11102976"},{"key":"e_1_2_15_36_2","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1111\/j.1865-1682.2011.01260.x","article-title":"First Evidence of peste des petits ruminants (PPR) Virus Circulation in Algeria (Sahrawi Territories): Outbreak Investigation and Virus Lineage Identification","volume":"59","author":"De Nardi M.","year":"2012","journal-title":"Transboundary and Emerging Diseases"},{"key":"e_1_2_15_37_2","doi-asserted-by":"publisher","DOI":"10.1017\/s0950268805003729"},{"key":"e_1_2_15_38_2","doi-asserted-by":"crossref","first-page":"192","DOI":"10.14737\/journal.aavs\/2014\/2.4.192.198","article-title":"Epidemiology and Diagnosis of Peste Des Petits Ruminants in Sheep and Goats by Serological, Molecular, and Isolation Methods in Gujarat, India","volume":"2","author":"Harshad C. 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A.","year":"2010","journal-title":"Scientific and Technical Review of the Office International des Epizooties"},{"key":"e_1_2_15_52_2","doi-asserted-by":"publisher","DOI":"10.20506\/rst.23.3.1522"},{"key":"e_1_2_15_53_2","article-title":"Peste Des Petits Ruminants (PPR) in Ethiopia: Analysis of a National Serological Survey","volume":"4","author":"Roger F.","year":"2008","journal-title":"BMC Veterinary Research"},{"key":"e_1_2_15_54_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11250-008-9300-9"},{"key":"e_1_2_15_55_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2005.02.011"}],"container-title":["Veterinary Medicine International"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/vmi\/9956146","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/vmi\/9956146","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/vmi\/9956146","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T06:49:34Z","timestamp":1777272574000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/vmi\/9956146"}},"subtitle":[],"editor":[{"given":"Lipi","family":"Buch","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":55,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/vmi\/9956146"],"URL":"https:\/\/doi.org\/10.1155\/vmi\/9956146","archive":["Portico"],"relation":{},"ISSN":["2090-8113","2042-0048"],"issn-type":[{"value":"2090-8113","type":"print"},{"value":"2042-0048","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-09-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-03","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"9956146"}}