{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T15:39:47Z","timestamp":1765294787677},"reference-count":0,"publisher":"Hindawi Limited","issue":"11","license":[{"start":{"date-parts":[[2003,1,1]],"date-time":"2003-01-01T00:00:00Z","timestamp":1041379200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Canadian Journal of Gastroenterology"],"published-print":{"date-parts":[[2003]]},"abstract":"<jats:p>AIMS: To evaluate the oxidative stress parameters before, during and after interferon treatment.<\/jats:p><jats:p>PATIENTS\/METHODS: Twenty patients were treated with interferon \u03b12b 5 MU, three times a week, subcutaneously, for 12 months. Liver biopsy was performed six months before treatment and at the six month follow-up. Chromosomal breakage studies were evaluated by the adjusted clastogenic score (ACS, normal value [nv] 1.1\u00b12.4%). Plasma malondialdehyde (MDA) was measured according to the Yagi method (nv 6.6\u00b11.4 nmol\/mL) and total thiols using the Ellman\u2019s reagent (DTNB) (nv 9.8\u00b11.3 \u00b5mol\/g protein). A serum marker of fibrogenesis, the amino-terminal propeptide of Procollagen type III (PIIIP), was quantified by radioimmunoassay (nv 0.37\u00b10.18 U\/L).<\/jats:p><jats:p>RESULTS: Compared with reference samples, the plasma of patients before treatment showed an increase of ACS (9.2\u00b13.2%, P&lt;0.001); higher MDA values (12.6\u00b12.7nmol\/mL, P&lt;0.001) and total plasma sulfhydryl groups (t-SH) were decreased (6.3\u00b11.1 \u00b5mol\/g protein, P&lt;0.001). During treatment and at the follow-up, a decrease in ACS was noticed in all patients (P&lt;0.001), but without normalization; a decrease in MDA was seen, with progressive normalization until the end of the follow up only in sustained responders (P&lt;0.003), while an increase of t-SH was seen, with progressive normalization until the end of follow up in all patients (P&lt;0.005). A positive correlation of ACS with grading of inflammation was found (r=0.52, P&lt;0.03) but not with fibrosis staging. In contrast, plasma MDA correlates with fibrosis staging (r=0.51, P&lt;0.03) and with PIIIP (r=0.57, P&lt;0.03) but without grading of inflammation.<\/jats:p><jats:p>CONCLUSIONS: The present study confirmed the presence of oxidative stress in chronic hepatitis C patients. Interferon promotes a long term inhibition of oxidative stress with concomitant improvement of activity and fibrosis. In the management of chronic hepatitis C, adjuvant therapy with antioxidants could be useful.<\/jats:p>","DOI":"10.1155\/2003\/710693","type":"journal-article","created":{"date-parts":[[2016,3,20]],"date-time":"2016-03-20T21:01:51Z","timestamp":1458507711000},"page":"644-650","source":"Crossref","is-referenced-by-count":30,"title":["Oxidative Stress in Chronic Hepatitis C: The Effect of Interferon Therapy and Correlation with Pathological Features"],"prefix":"10.1155","volume":"17","author":[{"given":"F\u00e1tima","family":"Serejo","sequence":"first","affiliation":[{"name":"Centre of Gastroenterology (Institute of Molecular Medicine), University of Lisbon, Lisbon, Portugal"}]},{"given":"Ingrid","family":"Emerit","sequence":"additional","affiliation":[{"name":"Institut Biom\u00e9dical des Cordeliers, University Paris VI, Paris, France"}]},{"given":"Paulo Manuel","family":"Filipe","sequence":"additional","affiliation":[{"name":"Centre of Metabolism and Endocrinology, University of Lisbon, Lisbon, Portugal"}]},{"given":"Afonso Camilo","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Centre of Metabolism and Endocrinology, University of Lisbon, Lisbon, Portugal"},{"name":"Department of Pathology, Santa Maria Hospital, University of Lisbon, Lisbon, Portugal"}]},{"given":"Maria Ad\u00edlia","family":"Costa","sequence":"additional","affiliation":[{"name":"Department of Pathology, Santa Maria Hospital, University of Lisbon, Lisbon, Portugal"}]},{"given":"Jo\u00e3o Pedro","family":"Freitas","sequence":"additional","affiliation":[{"name":"Centre of Metabolism and Endocrinology, University of Lisbon, Lisbon, Portugal"}]},{"given":"Miguel Carneiro","family":"de Moura","sequence":"additional","affiliation":[{"name":"Centre of Gastroenterology (Institute of Molecular Medicine), University of Lisbon, Lisbon, Portugal"}]}],"member":"98","container-title":["Canadian Journal of Gastroenterology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/cjgh\/2003\/710693.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/cjgh\/2003\/710693.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2016,8,23]],"date-time":"2016-08-23T14:11:16Z","timestamp":1471961476000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/cjgh\/2003\/710693\/abs\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003]]},"references-count":0,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2003]]}},"alternative-id":["710693"],"URL":"https:\/\/doi.org\/10.1155\/2003\/710693","relation":{},"ISSN":["0835-7900"],"issn-type":[{"value":"0835-7900","type":"print"}],"subject":[],"published":{"date-parts":[[2003]]}}}