{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:28:38Z","timestamp":1740169718005,"version":"3.37.3"},"reference-count":9,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of<jats:italic>Triatoma infestans<\/jats:italic>(Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of triatomines. The identification method proved to be quite sensitive, even without the extraction and purification of RNA virus.<\/jats:p>","DOI":"10.1155\/2013\/218593","type":"journal-article","created":{"date-parts":[[2013,4,4]],"date-time":"2013-04-04T18:28:34Z","timestamp":1365100114000},"page":"1-3","source":"Crossref","is-referenced-by-count":3,"title":["Molecular Techniques for Dicistrovirus Detection without RNA Extraction or Purification"],"prefix":"10.1155","volume":"2013","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1438-1268","authenticated-orcid":true,"given":"Jailson F. 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