{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T20:25:54Z","timestamp":1747427154506},"reference-count":29,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Portuguese Foundation for Science and Technology","award":["PTDC\/EEB-BIO\/99810\/2008","PTDC\/SAU-MET\/111398\/2009","REDE\/1517\/RMN\/2005"],"award-info":[{"award-number":["PTDC\/EEB-BIO\/99810\/2008","PTDC\/SAU-MET\/111398\/2009","REDE\/1517\/RMN\/2005"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>The<jats:sup>13<\/jats:sup>C-isotopomer enrichment of hepatic cytosolic acetyl-CoA of overnight-fed mice whose drinking water was supplemented with [U-<jats:sup>13<\/jats:sup>C]fructose, and [1-<jats:sup>13<\/jats:sup>C]glucose and<jats:italic>p<\/jats:italic>-amino benzoic acid (PABA) was quantified by<jats:sup>13<\/jats:sup>C NMR analysis of urinary<jats:italic>N<\/jats:italic>-acetyl-PABA. Four mice were given normal chow plus drinking water supplemented with 5% [1-<jats:sup>13<\/jats:sup>C]glucose, 2.5% [U-<jats:sup>13<\/jats:sup>C]fructose, and 2.5% fructose (Solution 1) overnight. Four were given chow and water containing 17.5% [1-<jats:sup>13<\/jats:sup>C]glucose, 8.75% [U-<jats:sup>13<\/jats:sup>C]fructose and 8.75% fructose (Solution 2). PABA (0.25%) was present in both studies. Urinary<jats:italic>N<\/jats:italic>-acetyl-PABA was analyzed by<jats:sup>13<\/jats:sup>C NMR. In addition to [2-<jats:sup>13<\/jats:sup>C]- and [1,2-<jats:sup>13<\/jats:sup>C]acetyl isotopomers from catabolism of [U-<jats:sup>13<\/jats:sup>C]fructose and [1-<jats:sup>13<\/jats:sup>C]glucose to acetyl-CoA, [1-<jats:sup>13<\/jats:sup>C]acetyl was also found indicating pyruvate recycling activity. This precluded precise estimates of [1-<jats:sup>13<\/jats:sup>C]glucose contribution to acetyl-CoA while that of [U-<jats:sup>13<\/jats:sup>C]fructose was unaffected. The fructose contribution to acetyl-CoA from Solutions 1 and 2 was 4.0 \u00b1 0.4% and 10.6 \u00b1 0.6%, respectively, indicating that it contributed to a minor fraction of lipogenic acetyl-CoA under these conditions.<\/jats:p>","DOI":"10.1155\/2013\/638085","type":"journal-article","created":{"date-parts":[[2013,6,10]],"date-time":"2013-06-10T21:01:13Z","timestamp":1370898073000},"page":"1-7","source":"Crossref","is-referenced-by-count":6,"title":["Noninvasive Measurement of Murine Hepatic Acetyl-CoA<sup>13<\/sup>C-Enrichment Following Overnight Feeding with<sup>13<\/sup>C-Enriched Fructose and Glucose"],"prefix":"10.1155","volume":"2013","author":[{"given":"Filipa","family":"Carvalho","sequence":"first","affiliation":[{"name":"Center for Neurosciences and Cell Biology, Department of Zoology, University of Coimbra, Coimbra 3004-517, Portugal"}]},{"given":"Joao","family":"Duarte","sequence":"additional","affiliation":[{"name":"Advanced Imaging Research Center, University of Texas 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