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The histological examination of the liver sections of rats showed that prominent hepatocytes with marked vacuolisation and inflammatory cells which were arranged around the necrotic tissue are more at 96\u2009h after exposure to CCl<jats:sub>4<\/jats:sub>. Moreover maximum increase in GR and GP activities, 2.1 and 2.5 times, respectively, was observed at 96\u2009h after exposure to CCl<jats:sub>4<\/jats:sub>, what coincided with the maximum of free radical oxidation processes. Using a combination of reverse transcription and real-time polymerase chain reaction, expression of the glutathione peroxidase and glutathione reductase genes (Gpx1 and Gsr) was analyzed by the determination of their respective mRNAs in the rat liver tissue under toxic hepatitis conditions. The analyses of Gpx1 and Gsr expression revealed that the transcript levels increased in 2.5- and 3.0-folds, respectively. Western blot analysis revealed that the amounts of hepatic Gpx1 and Gsr proteins increased considerably after CCl<jats:sub>4<\/jats:sub>administration. It can be proposed that the overexpression of these enzymes could be a mechanism of enhancement of hepatocytes tolerance to oxidative stress.<\/jats:p>","DOI":"10.1155\/2013\/870628","type":"journal-article","created":{"date-parts":[[2013,3,11]],"date-time":"2013-03-11T21:01:08Z","timestamp":1363035668000},"page":"1-9","source":"Crossref","is-referenced-by-count":32,"title":["Expression of Glutathione Peroxidase and Glutathione Reductase and Level of Free Radical Processes under Toxic Hepatitis in Rats"],"prefix":"10.1155","volume":"2013","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4409-1077","authenticated-orcid":true,"given":"Igor Y.","family":"Iskusnykh","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, Voronezh State University, University Square 1, Voronezh 394006, Russia"}]},{"given":"Tatyana N.","family":"Popova","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, Voronezh State University, University Square 1, Voronezh 394006, Russia"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5774-7971","authenticated-orcid":true,"given":"Aleksander A.","family":"Agarkov","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, Voronezh State University, University Square 1, Voronezh 394006, Russia"}]},{"given":"Miguel \u00c2. 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