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In the present study, the effect of human MSCs from Wharton\u2019s jelly (HMSCs), differentiated into neuroglial-like cells associated to poly (DL-lactide-<jats:italic>\u03b5<\/jats:italic>-caprolactone) membrane, on nerve regeneration, was evaluated in the neurotmesis injury rat sciatic nerve model. Results<jats:italic>in vitro<\/jats:italic>showed successful differentiation of HMSCs into neuroglial-like cells, characterized by expression of specific neuroglial markers confirmed by immunocytochemistry and by RT-PCR and qPCR targeting specific genes expressed.<jats:italic>In vivo<\/jats:italic>testing evaluated during the healing period of 20 weeks, showed no evident positive effect of HMSCs or neuroglial-like cell enrichment at the sciatic nerve repair site on most of the functional and nerve morphometric predictors of nerve regeneration although the nociception function was almost normal. EPT on the other hand, recovered significantly better after HMSCs enriched membrane employment, to values of residual functional impairment compared to other treated groups. When the neurotmesis injury can be surgically reconstructed with an end-to-end suture or by grafting, the addition of a PLC membrane associated with HMSCs seems to bring significant advantage, especially concerning the motor function recovery.<\/jats:p>","DOI":"10.1155\/2014\/302659","type":"journal-article","created":{"date-parts":[[2014,7,10]],"date-time":"2014-07-10T21:10:18Z","timestamp":1405026618000},"page":"1-17","source":"Crossref","is-referenced-by-count":23,"title":["Promoting Nerve Regeneration in a Neurotmesis Rat Model Using Poly(DL-lactide-<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi mathvariant=\"bold-italic\">\u03b5<\/mml:mi><\/mml:mrow><\/mml:math>-caprolactone) Membranes and Mesenchymal Stem Cells from the Wharton\u2019s Jelly:<i>In Vitro<\/i>and<i>In Vivo<\/i>Analysis"],"prefix":"10.1155","volume":"2014","author":[{"given":"T.","family":"Pereira","sequence":"first","affiliation":[{"name":"Departamento de Cl\u00ednicas Veterin\u00e1rias, Instituto de Ci\u00eancias Biom\u00e9dicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira No. 228, 4050-313 Porto, Portugal"},{"name":"Centro de Estudos de Ci\u00eancia Animal (CECA), Instituto de Ci\u00eancias e Tecnologias Agr\u00e1rias e Agro-Alimentares (ICETA), Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal"}]},{"given":"A.","family":"G\u00e4rtner","sequence":"additional","affiliation":[{"name":"Centro de Estudos de Ci\u00eancia Animal (CECA), Instituto de Ci\u00eancias e Tecnologias Agr\u00e1rias e Agro-Alimentares (ICETA), Rua D. 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