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The eye is a good target due to its small size, minimal diffusion of therapeutic agent to the systemic circulation, and low immune and inflammatory responses. Currently, most approaches are based on viral vectors, but efforts continue towards the synthesis and evaluation of new nonviral carriers to improve nucleic acid delivery. Our objective is to evaluate the efficiency of novel cationic retinoic and carotenoic glycol phospholipids, designated C20-18, C20-20, and C30-20, to deliver DNA to human retinal pigmented epithelium (RPE) cells. Liposomes were produced by solvent evaporation of ethanolic mixtures of the polyene compounds and coformulated with 1,2-dioleoyl-<jats:italic>sn<\/jats:italic>-glycero-3-phosphoethanolamine (DOPE) or cholesterol (Chol). Addition of DNA to the liposomes formed lipoplexes, which were characterized for binding, size, biocompatibility, and transgene efficiency. Lipoplex formulations of suitable size and biocompatibility were assayed for DNA delivery, both qualitatively and quantitatively, using RPE cells and a GFP-encoding plasmid. The retinoic lipoplex formulation with DOPE revealed a transfection efficiency comparable to the known lipid references 3<jats:italic>\u03b2<\/jats:italic>-[<jats:italic>N<\/jats:italic>-(<jats:italic>N<\/jats:italic>\u2032,<jats:italic>N<\/jats:italic>\u2032-dimethylaminoethane)-carbamoyl]-cholesterol (DC-Chol) and 1,2-dioleoyl-<jats:italic>sn<\/jats:italic>-glycero-3-ethylphosphocholine (EPC) and GeneJuice. The results demonstrate that cationic polyene phospholipids have potential as DNA carriers for ocular gene therapy.<\/jats:p>","DOI":"10.1155\/2014\/703253","type":"journal-article","created":{"date-parts":[[2014,7,24]],"date-time":"2014-07-24T18:01:27Z","timestamp":1406224887000},"page":"1-13","source":"Crossref","is-referenced-by-count":7,"title":["Cationic Polyene Phospholipids as DNA Carriers for Ocular Gene Therapy"],"prefix":"10.1155","volume":"2014","author":[{"given":"Susana","family":"Machado","sequence":"first","affiliation":[{"name":"Center for Biomedicine and Structural Biology (CBME), Institute for Biotechnology and Bioengineering (IBB), University of Algarve, 8005-139 Faro, Portugal"}]},{"given":"Sofia","family":"Calado","sequence":"additional","affiliation":[{"name":"Center for Biomedicine and Structural Biology (CBME), Institute for Biotechnology and Bioengineering (IBB), University of Algarve, 8005-139 Faro, Portugal"},{"name":"Doctoral Program in Biomedical Sciences, Department of Biomedical Sciences and Medicine, University of Algarve, 8005-139 Faro, Portugal"}]},{"given":"Diogo","family":"Bitoque","sequence":"additional","affiliation":[{"name":"Center for Biomedicine and Structural Biology (CBME), Institute for Biotechnology and Bioengineering (IBB), University of Algarve, 8005-139 Faro, Portugal"},{"name":"ProRegeM Doctoral Program, Department of Biomedical Sciences and Medicine, University of Algarve, 8005-139 Faro, Portugal"}]},{"given":"Ana Vanessa","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Center for Biomedicine and Structural Biology (CBME), Institute for Biotechnology and Bioengineering (IBB), University of Algarve, 8005-139 Faro, Portugal"},{"name":"Doctoral Program in Biomedical Sciences, Department of Biomedical Sciences and Medicine, University of Algarve, 8005-139 Faro, Portugal"}]},{"given":"Christer L.","family":"\u00d8pstad","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Norwegian University of Science and Technology, 7491 Trondheim, Norway"}]},{"given":"Muhammad","family":"Zeeshan","sequence":"additional","affiliation":[{"name":"Premedical Unit, Weill Cornell Medical College in Qatar, P.O. 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