{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T18:58:28Z","timestamp":1773601108352,"version":"3.50.1"},"reference-count":23,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. This work compares the osteoblastic behaviour of a bone marrow (BM) aspirate and a prepared BM concentrate of nucleated cells associated with a glass reinforced hydroxyapatite composite (GRHC) in a microporous pellet formulation.<jats:italic>Methods<\/jats:italic>. BM aspirate (30\u2009mL) was collected during 3 orthopedic surgical procedures, and a concentration system was used to achieve 3 rapid preparations of a concentrate of nucleated cells (3\u2009mL) from the BM aspirates. The BM aspirates (53% cell viability;<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mn>2.7<\/mml:mn><mml:mo>\u00d7<\/mml:mo><mml:msup><mml:mrow><mml:mn>10<\/mml:mn><\/mml:mrow><mml:mrow><mml:mn mathvariant=\"normal\">6<\/mml:mn><\/mml:mrow><\/mml:msup><\/mml:math>nucleated cell\/mL) and the BM concentrates (76% cell viability;<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mn>2<\/mml:mn><mml:mo>\u00d7<\/mml:mo><mml:msup><mml:mrow><mml:mn>10<\/mml:mn><\/mml:mrow><mml:mrow><mml:mn mathvariant=\"normal\">7<\/mml:mn><\/mml:mrow><\/mml:msup><\/mml:math>nucleated cell\/mL) were cultured over glass reinforced hydroxyapatite pellets, at the same volume\/mass ratio, for 30 days. Cultures performed in standard tissue culture plates were used as control.<jats:italic>Results<\/jats:italic>. The colonized BM concentrate\/material constructs exhibited a representative osteoblastic proliferation\/differentiation pathway, evidenced by a high alkaline phosphatase (ALP) activity, expression of collagen type 1, ALP, BMP-2, M-CSF, RANKL, and OPG, and formation of a calcium phosphate mineralized matrix. A clear improved behaviour was noticed compared to the BM aspirate\/material constructs.<jats:italic>Conclusions<\/jats:italic>. The results suggest the benefit of using an autologous BM concentrate\/material construct in the clinical setting, in bone regeneration applications.<\/jats:p>","DOI":"10.1155\/2015\/240698","type":"journal-article","created":{"date-parts":[[2015,1,22]],"date-time":"2015-01-22T21:00:49Z","timestamp":1421960449000},"page":"1-10","source":"Crossref","is-referenced-by-count":1,"title":["The Benefit of a Human Bone Marrow Stem Cells Concentrate in addition to an Inorganic Scaffold for Bone Regeneration: An<i>In Vitro<\/i>Study"],"prefix":"10.1155","volume":"2015","author":[{"given":"J.","family":"Torres","sequence":"first","affiliation":[{"name":"Faculdade de Medicina da Universidade do Porto (FMUP), Hospital de S\u00e3o Jo\u00e3o, Largo Hern\u00e2ni Monteiro, 4200 Porto, Portugal"}]},{"given":"A.","family":"Lopes","sequence":"additional","affiliation":[{"name":"CEMUC, Departamento de Engenharia Metal\u00fargica e Materiais, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"given":"M. A.","family":"Lopes","sequence":"additional","affiliation":[{"name":"CEMUC, Departamento de Engenharia Metal\u00fargica e Materiais, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]},{"given":"M.","family":"Gutierres","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina da Universidade do Porto (FMUP), Hospital de S\u00e3o Jo\u00e3o, Largo Hern\u00e2ni Monteiro, 4200 Porto, Portugal"}]},{"given":"A. T.","family":"Cabral","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina da Universidade do Porto (FMUP), Hospital de S\u00e3o Jo\u00e3o, Largo Hern\u00e2ni Monteiro, 4200 Porto, Portugal"}]},{"given":"M. H.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina Dent\u00e1ria da Universidade do Porto (FMDUP), Rua Dr. Manuel Pereira da Silva, 4200-393 Porto, Portugal"}]},{"given":"E.","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina da Universidade do Porto (FMUP), Hospital de S\u00e3o Jo\u00e3o, Largo Hern\u00e2ni Monteiro, 4200 Porto, Portugal"}]},{"given":"C. F.","family":"van Eck","sequence":"additional","affiliation":[{"name":"University of Pittsburgh Medical Center (UPMC), Kaufman Building, Suite 1011, Pittsburgh, PA 15213, USA"}]},{"given":"J. D.","family":"Santos","sequence":"additional","affiliation":[{"name":"CEMUC, Departamento de Engenharia Metal\u00fargica e Materiais, Faculdade de Engenharia, Universidade do Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal"}]}],"member":"98","reference":[{"issue":"12","key":"1","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.3928\/0147-7447-19951201-05","volume":"18","year":"1995","journal-title":"Orthopedics"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1002\/mabi.200400026"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-1383(08)70003-2"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-1383(08)70010-X"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1097\/00007632-199505000-00012"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/S0030-5898(05)70110-3"},{"issue":"7","key":"7","doi-asserted-by":"crossref","first-page":"971","DOI":"10.2106\/00004623-200107000-00001","volume":"83","year":"2001","journal-title":"Journal of Bone and Joint Surgery Series A"},{"key":"8","first-page":"68","volume-title":"Clinical perspectives on the use of bone graft based on allografts","year":"2003"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.b.31884"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(99)00112-X"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/S0142-9612(99)00248-3"},{"key":"27","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1002\/1097-4636(20010315)54:4<463::AID-JBM10>3.0.CO;2-Y","volume":"54","year":"2001","journal-title":"Journal of Biomedical Materials Research"},{"key":"28","first-page":"477","volume-title":"Bonelike graft for bone regenerative applications","year":"2006"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2004-0308"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/j.cuor.2007.07.004"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1002\/stem.269"},{"issue":"276","key":"29","first-page":"291","year":"1992","journal-title":"Clinical Orthopaedics and Related Research"},{"key":"30","first-page":"85","volume-title":"Mesenchymal stem cell and osteoblast differentiation","year":"2008"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1210\/er.21.4.393"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2008.03.027"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1038\/nature01658"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1530\/EJE-11-0132"},{"issue":"268","key":"21","first-page":"294","year":"1991","journal-title":"Clinical Orthopaedics and Related Research"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/240698.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/240698.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2015\/240698.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,27]],"date-time":"2022-04-27T07:58:48Z","timestamp":1651046328000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2015\/240698\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":23,"alternative-id":["240698","240698"],"URL":"https:\/\/doi.org\/10.1155\/2015\/240698","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}