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Measurement of endogenous glucose production (EGP) and Cori cycle activity provide insights into the maintenance of glycemic control in these animals. These parameters were determined in 7 wild-type (ATGL<jats:sup>+\/\u2212<\/jats:sup>) and 6 ATGL<jats:sup>\u2212\/\u2212<\/jats:sup>mice by a primed-infusion of [U-<jats:sup>13<\/jats:sup>C<jats:sub>6<\/jats:sub>]glucose followed by LC-MS\/MS targeted mass-isotopomer analysis of blood glucose. EGP was quantified by isotope dilution of [U-<jats:sup>13<\/jats:sup>C<jats:sub>6<\/jats:sub>]glucose while Cori cycling was estimated by analysis of glucose triose<jats:sup>13<\/jats:sup>C-isotopomers. Fasting plasma free fatty-acids were significantly lower in ATGL<jats:sup>\u2212\/\u2212<\/jats:sup>versus control mice (0.43 \u00b1 0.05\u2009mM<jats:italic>versus<\/jats:italic>0.73 \u00b1 0.11\u2009mM,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>). Six-hour fasting EGP rates were identical for both ATGL<jats:sup>\u2212\/\u2212<\/jats:sup>and control mice (79 \u00b1 11<jats:italic>versus<\/jats:italic>71 \u00b1 7\u2009<jats:italic>\u03bc<\/jats:italic>mol\/kg\/min, resp.). Peripheral glucose metabolism was dominated by Cori cycling (80 \u00b1 2% and 82 \u00b1 7% of glucose disposal for ATGL<jats:sup>\u2212\/\u2212<\/jats:sup>and control mice, resp.) indicating that peripheral glucose oxidation was not significantly upregulated in ATGL<jats:sup>\u2212\/\u2212<\/jats:sup>mice under these conditions. The glucose<jats:sup>13<\/jats:sup>C-isotopomer distributions in both ATGL<jats:sup>\u2212\/\u2212<\/jats:sup>and control mice were consistent with extensive hepatic pyruvate recycling. This suggests that gluconeogenic outflow from the Krebs cycle was also well compensated in ATGL<jats:sup>\u2212\/\u2212<\/jats:sup>mice.<\/jats:p>","DOI":"10.1155\/2015\/542029","type":"journal-article","created":{"date-parts":[[2015,7,5]],"date-time":"2015-07-05T21:01:38Z","timestamp":1436130098000},"page":"1-8","source":"Crossref","is-referenced-by-count":6,"title":["Effect of Global ATGL Knockout on Murine Fasting Glucose Kinetics"],"prefix":"10.1155","volume":"2015","author":[{"given":"Margarida","family":"Coelho","sequence":"first","affiliation":[{"name":"CNC\u2014Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal"}]},{"given":"Patricia","family":"Nunes","sequence":"additional","affiliation":[{"name":"MRC National Institute for Medical Research, London, UK"}]},{"given":"Vera M.","family":"Mendes","sequence":"additional","affiliation":[{"name":"CNC\u2014Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal"}]},{"given":"Bruno","family":"Manadas","sequence":"additional","affiliation":[{"name":"CNC\u2014Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal"}]},{"given":"Arend","family":"Heerschap","sequence":"additional","affiliation":[{"name":"Department of Radiology, Radboud University Nijmegen Medical Centre, Nijmegen, Netherlands"}]},{"given":"John G.","family":"Jones","sequence":"additional","affiliation":[{"name":"CNC\u2014Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal"},{"name":"Portuguese Diabetes Association (APDP), Lisbon, Portugal"}]}],"member":"311","reference":[{"issue":"42","key":"1","first-page":"8","volume":"95","year":"2002","journal-title":"Journal of the Royal Society of 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