{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T08:01:49Z","timestamp":1768809709074,"version":"3.49.0"},"reference-count":97,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Associa\u00e7\u00e3o Portuguesa Contra a Leucemia"},{"name":"Forum Hematol\u00f3gico do Norte"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2015]]},"abstract":"Studies of chemokine receptors (CKR) in natural killer- (NK-) cells have already been published, but only a few gave detailed information on its differential expression on blood NK-cell subsets. We report on the expression of the inflammatory and homeostatic CKR on normal bloodCD56<\/mml:mtext><\/mml:mrow>+<\/mml:mo>low<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>CD16+<\/jats:sup>andCD56<\/mml:mtext><\/mml:mrow>+<\/mml:mo>high<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>\u2009\u2009CD16<\/mml:mtext><\/mml:mrow>-<\/mml:mo>\/<\/mml:mo>+<\/mml:mo>low<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>NK-cells. ConventionalCD56<\/mml:mtext><\/mml:mrow>+<\/mml:mo>low<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>andCD56<\/mml:mtext><\/mml:mrow>+<\/mml:mo>high<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>NK-cells present in the normal PB do express CKR for inflammatory cytokines, although with different patternsCD56<\/mml:mtext><\/mml:mrow>+<\/mml:mo>low<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>NK-cells are mainly CXCR1\/CXCR2+<\/jats:sup>and CXCR3\/CCR5\u2212\/+<\/jats:sup>, whereas mostlyCD56<\/mml:mtext><\/mml:mrow>+<\/mml:mo>high<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>NK-cells are CXCR1\/CXCR2\u2212<\/jats:sup>and CXCR3\/CCR5+<\/jats:sup>. Both NK-cell subsets have variable CXCR4 expression and are CCR4\u2212<\/jats:sup>and CCR6\u2212<\/jats:sup>. The CKR repertoire of theCD56<\/mml:mtext><\/mml:mrow>+<\/mml:mo>low<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>NK-cells approaches to that of neutrophils, whereas the CKR repertoire of theCD56<\/mml:mtext><\/mml:mrow>+<\/mml:mo>high<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>NK-cells mimics that of Th1+<\/jats:sup>T cells, suggesting that these cells are prepared to migrate into inflamed tissues at different phases of the immune response. In addition, we describe a subpopulation of NK-cells with intermediate levels of CD56 expression, which we namedCD56<\/mml:mtext><\/mml:mrow>+<\/mml:mo>int<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>NK-cells. These NK-cells are CXCR3\/CCR5+<\/jats:sup>, they have intermediate levels of expression of CD16, CD62L, CD94, and CD122, and they are CD57\u2212<\/jats:sup>and CD158a\u2212<\/jats:sup>. In view of their phenotypic features, we hypothesize that they correspond to a transitional stage, between the well-knownCD56<\/mml:mtext><\/mml:mrow>+<\/mml:mo>high<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>andCD56<\/mml:mtext><\/mml:mrow>+<\/mml:mo>low<\/mml:mtext><\/mml:mrow><\/mml:msup><\/mml:mrow><\/mml:math>NK-cells populations.<\/jats:p>","DOI":"10.1155\/2015\/839684","type":"journal-article","created":{"date-parts":[[2015,10,13]],"date-time":"2015-10-13T20:47:57Z","timestamp":1444769277000},"page":"1-18","source":"Crossref","is-referenced-by-count":49,"title":["Chemokine Receptor Expression on Normal Blood CD56+<\/sup>NK-Cells Elucidates Cell Partners That Comigrate during the Innate and Adaptive Immune Responses and Identifies a Transitional NK-Cell Population"],"prefix":"10.1155","volume":"2015","author":[{"given":"Margarida","family":"Lima","sequence":"first","affiliation":[{"name":"Laboratory of Cytometry, Service of Hematology, Hospital de Santo Ant\u00f3nio (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, Portugal"}]},{"given":"Magdalena","family":"Leander","sequence":"additional","affiliation":[{"name":"Laboratory of Cytometry, Service of Hematology, Hospital de Santo Ant\u00f3nio (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, Portugal"}]},{"given":"Marlene","family":"Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Cytometry, Service of Hematology, Hospital de Santo Ant\u00f3nio (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, Portugal"}]},{"given":"Ana Helena","family":"Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Cytometry, Service of Hematology, Hospital de Santo Ant\u00f3nio (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, Portugal"}]},{"given":"Catarina","family":"Lau","sequence":"additional","affiliation":[{"name":"Laboratory of Cytometry, Service of Hematology, Hospital de Santo Ant\u00f3nio (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, Portugal"}]},{"given":"Maria Lu\u00eds","family":"Queir\u00f3s","sequence":"additional","affiliation":[{"name":"Laboratory of Cytometry, Service of Hematology, Hospital de Santo Ant\u00f3nio (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, Portugal"}]},{"given":"Marta","family":"Gon\u00e7alves","sequence":"additional","affiliation":[{"name":"Laboratory of Cytometry, Service of Hematology, Hospital de Santo Ant\u00f3nio (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, Portugal"}]},{"given":"S\u00f3nia","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Laboratory of Cytometry, Service of Hematology, Hospital de Santo Ant\u00f3nio (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, Portugal"}]},{"given":"Jo\u00e3o","family":"Moura","sequence":"additional","affiliation":[{"name":"Laboratory of Cytometry, Service of Hematology, Hospital de Santo Ant\u00f3nio (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, Portugal"}]},{"given":"Maria dos Anjos","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Laboratory of Cytometry, Service of Hematology, Hospital de Santo Ant\u00f3nio (HSA), Centro Hospitalar do Porto (CHP), Rua D. Manuel II, 4050-345 Porto, Portugal"}]},{"given":"Alberto","family":"Orfao","sequence":"additional","affiliation":[{"name":"Laboratory of Flow Cytometry, Centro de Investigaci\u00f3n del Cancer (CIC), Campus Miguel de Unamuno, 37007 Salamanca, Spain"}]}],"member":"98","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1582"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1549"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.2741\/3165"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4402170"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2014.11.001"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0510250"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-03-453217"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.19.1.197"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-5890(01)00107-9"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.20.100301.064812"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1007\/82_2010_20"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2014.11.003"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1097-0215(19981123)78:5x003C;533::aid-ijc1x0003e;3.0.co;2-2"},{"issue":"4","key":"14","doi-asserted-by":"crossref","first-page":"431","DOI":"10.2174\/1566524043360609","volume":"4","year":"2004","journal-title":"Current Molecular Medicine"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00347"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1615\/critrevoncog.2014010386"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1007\/s12026-013-8477-9"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2014.01.010"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/S1472-6483(10)60575-5"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1172\/jci68107"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-04-568451"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1155\/2009\/405016"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(01)02060-9"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2008.03027.x"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830220102"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830270203"},{"issue":"1","key":"27","doi-asserted-by":"crossref","first-page":"400","DOI":"10.4049\/jimmunol.161.1.400","volume":"161","year":"1998","journal-title":"The Journal of Immunology"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(2001010)31:10x003C;3121::aid-immu3121x0003e;3.0.co;2-4"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1006\/bcmd.2001.0443"},{"issue":"6","key":"30","doi-asserted-by":"crossref","first-page":"923","DOI":"10.1002\/jlb.66.6.923","volume":"66","year":"1999","journal-title":"Journal of Leukocyte Biology"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.040569797"},{"issue":"10","key":"32","doi-asserted-by":"crossref","first-page":"3183","DOI":"10.4049\/jimmunol.143.10.3183","volume":"143","year":"1989","journal-title":"Journal of Immunology"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1182\/blood.v97.10.3146"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-08-238469"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1038\/35040504"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200324533"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.1.88"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.3.1455"},{"issue":"8","key":"39","doi-asserted-by":"crossref","first-page":"4947","DOI":"10.4049\/jimmunol.178.8.4947","volume":"178","year":"2007","journal-title":"Journal of Immunology"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.1.89"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1392.001"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.3.2227"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2003.00067.x"},{"issue":"1","key":"44","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/S1043-4666(02)00493-3","volume":"21","year":"2003","journal-title":"Cytokine"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.11.6477"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.11.7833"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-09-2876"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200425100"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2004.02.006"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2010.158"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.12.2009"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1521-4141(199811)28:11x003C;3696::aid-immu3696x0003e;3.3.co;2-n"},{"issue":"1","key":"53","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1002\/jlb.60.1.147","volume":"60","year":"1996","journal-title":"Journal of Leukocyte Biology"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.29.17161"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1038\/382829a0"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1038\/382833a0"},{"issue":"1","key":"57","first-page":"299","volume":"19","year":"2004","journal-title":"Histology and Histopathology"},{"key":"58","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.1.129"},{"key":"59","doi-asserted-by":"publisher","DOI":"10.1038\/23495"},{"key":"60","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.23.15036"},{"key":"61","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.3.1764"},{"issue":"1","key":"62","doi-asserted-by":"crossref","first-page":"186","DOI":"10.4049\/jimmunol.162.1.186","volume":"162","year":"1999","journal-title":"Journal of Immunology"},{"key":"63","doi-asserted-by":"publisher","DOI":"10.1016\/s1359-6101(03)00049-2"},{"key":"64","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.12.1755"},{"key":"65","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1422"},{"issue":"1","key":"66","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1002\/jlb.57.1.180","volume":"57","year":"1995","journal-title":"Journal of Leukocyte Biology"},{"issue":"4","key":"67","first-page":"311","volume":"34","year":"1997","journal-title":"Seminars in Hematology"},{"issue":"5","key":"68","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1002\/jlb.67.5.699","volume":"67","year":"2000","journal-title":"Journal of Leukocyte Biology"},{"key":"69","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keg240"},{"key":"70","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200535243"},{"key":"71","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.05.00030805"},{"key":"72","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-6143.2005.00995.x"},{"key":"73","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.2005.01692.x"},{"key":"74","doi-asserted-by":"publisher","DOI":"10.1086\/423283"},{"key":"75","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.23239"},{"key":"76","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.10.6418"},{"issue":"8","key":"77","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1093\/intimm\/3.8.743","volume":"3","year":"1991","journal-title":"International Immunology"},{"key":"78","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030305"},{"key":"79","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2009.02.030"},{"key":"80","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.4.2231"},{"key":"81","doi-asserted-by":"publisher","DOI":"10.5551\/jat.9399"},{"key":"82","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-01-264903"},{"key":"83","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxq434"},{"key":"84","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2011.044578"},{"key":"85","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2013.07.003"},{"key":"86","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021381"},{"key":"87","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.2.367"},{"key":"88","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.5.761"},{"key":"89","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mn.7800196"},{"key":"90","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-04-215491"},{"key":"91","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-11-253286"},{"key":"92","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011966"},{"key":"93","doi-asserted-by":"publisher","DOI":"10.1006\/bcmd.2002.0506"},{"issue":"3-4","key":"94","first-page":"331","volume":"18","year":"2004","journal-title":"Journal of Biological Regulators & Homeostatic Agents"},{"key":"95","first-page":"274","volume-title":"Chronic Iymphoproliferative disorders of NK cells","year":"2008"},{"key":"96","doi-asserted-by":"publisher","DOI":"10.1016\/s0002-9440(10)63373-1"},{"key":"97","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2014.905772"}],"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2015\/839684.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2015\/839684.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2015\/839684.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,15]],"date-time":"2023-08-15T07:58:46Z","timestamp":1692086326000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/jir\/2015\/839684\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015]]},"references-count":97,"alternative-id":["839684","839684"],"URL":"https:\/\/doi.org\/10.1155\/2015\/839684","relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"value":"2314-8861","type":"print"},{"value":"2314-7156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015]]}}}