{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:35:40Z","timestamp":1760985340102,"version":"3.37.3"},"reference-count":21,"publisher":"Wiley","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Foundation for Cancer Research, Szeged","award":["4.2.2.A-11\/1\/KONV-2012-0035"],"award-info":[{"award-number":["4.2.2.A-11\/1\/KONV-2012-0035"]}]},{"DOI":"10.13039\/501100004895","name":"European Social Fund","doi-asserted-by":"publisher","award":["4.2.2.A-11\/1\/KONV-2012-0035"],"award-info":[{"award-number":["4.2.2.A-11\/1\/KONV-2012-0035"]}],"id":[{"id":"10.13039\/501100004895","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2015]]},"abstract":"<jats:p>Ecdysteroids, analogs of the insect molting hormone, are known for their various mild, nonhormonal bioactivities in mammals. Previously, we reported that less-polar ecdysteroids can modulate the doxorubicin resistance of a multidrug resistant (MDR) mouse lymphoma cell line expressing the human ABCB1 transporter. Here, we describe the ability of 20-hydroxyecdysone (<jats:bold>1<\/jats:bold>) and its mono- (<jats:bold>2<\/jats:bold>) and diacetonide (<jats:bold>3<\/jats:bold>) derivatives to sensitize various MDR and non-MDR cancer cell lines towards doxorubicin, paclitaxel, vincristine, or cisplatin. Drug IC<jats:sub>50<\/jats:sub>values with or without ecdysteroid were determined by MTT assay. Compound<jats:bold>3<\/jats:bold>significantly sensitized all cell lines to each chemotherapeutic except for cisplatin, whose activity was decreased. In order to overcome solubility and stability issues for the future<jats:italic>in vivo<\/jats:italic>administration of compound<jats:bold>3<\/jats:bold>, liposomal formulations were developed. By means of their combination index values obtained via checkerboard microplate method, a formulation showed superior activity to that of compound<jats:bold>3<\/jats:bold>alone. Because ecdysteroids act also on non-ABCB1 expressing (sensitive) cell lines, our results demonstrate that they do not or not exclusively exert their adjuvant anticancer activity as ABCB1 inhibitors, but other mechanisms must be involved, and they opened the way towards their<jats:italic>in vivo<\/jats:italic>bioactivity testing against various cancer xenografts.<\/jats:p>","DOI":"10.1155\/2015\/895360","type":"journal-article","created":{"date-parts":[[2015,5,18]],"date-time":"2015-05-18T02:52:37Z","timestamp":1431917557000},"page":"1-8","source":"Crossref","is-referenced-by-count":18,"title":["Ecdysteroids Sensitize MDR and Non-MDR Cancer Cell Lines to Doxorubicin, Paclitaxel, and Vincristine but Tend to Protect Them from Cisplatin"],"prefix":"10.1155","volume":"2015","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8541-7440","authenticated-orcid":true,"given":"Ana","family":"Martins","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged, D\u00f3m t\u00e9r 10, Szeged 6720, Hungary"},{"name":"Unidade de Parasitologia e Microbiologia M\u00e9dica, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira 100, 1349-008 Lisbon, Portugal"}]},{"given":"P\u00e9ter","family":"Sipos","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Szeged, E\u00f6tv\u00f6s Ucta 6, Szeged 6720, Hungary"}]},{"given":"Katalin","family":"D\u00e9r","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Szeged, E\u00f6tv\u00f6s Ucta 6, Szeged 6720, Hungary"}]},{"given":"J\u00f3zsef","family":"Cs\u00e1bi","sequence":"additional","affiliation":[{"name":"Institute of Pharmacognosy, Faculty of Pharmacy, University of Szeged, E\u00f6tv\u00f6s Ucta 6, Szeged 6720, Hungary"}]},{"given":"Walter","family":"Miklos","sequence":"additional","affiliation":[{"name":"Department of Medicine I, Institute of Cancer Research and Comprehensive Cancer Center, Medical University of Vienna, Borschkegasse 8A, 1090 Vienna, Austria"}]},{"given":"Walter","family":"Berger","sequence":"additional","affiliation":[{"name":"Department of Medicine I, Institute of Cancer Research and Comprehensive Cancer Center, Medical University of Vienna, Borschkegasse 8A, 1090 Vienna, Austria"},{"name":"Institute of Inorganic Chemistry and Research Platform \u201cTranslational Cancer Therapy Research\u201d, University of Vienna, Waehringer Strasse 42, 1090 Vienna, Austria"}]},{"given":"Attila","family":"Zalatnai","sequence":"additional","affiliation":[{"name":"First Department of Pathology and Experimental Cancer Research, Semmelweis University, \u00dcll\u0151i \u00fat 26, Budapest 1085, Hungary"}]},{"given":"Leonard","family":"Amaral","sequence":"additional","affiliation":[{"name":"Centro de Mal\u00e1ria e Outras Doen\u00e7as Tropicais (CMDT), Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira 100, 1349-008 Lisbon, Portugal"}]},{"given":"Joseph","family":"Moln\u00e1r","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunobiology, Faculty of Medicine, University of Szeged, D\u00f3m t\u00e9r 10, Szeged 6720, Hungary"}]},{"given":"Piroska","family":"Szab\u00f3-R\u00e9v\u00e9sz","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Szeged, E\u00f6tv\u00f6s Ucta 6, Szeged 6720, Hungary"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0074-3472","authenticated-orcid":true,"given":"Attila","family":"Hunyadi","sequence":"additional","affiliation":[{"name":"Institute of Pharmacognosy, Faculty of Pharmacy, University of Szeged, 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